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Q91293

- CPSM_LITCT

UniProt

Q91293 - CPSM_LITCT

Protein

Carbamoyl-phosphate synthase [ammonia], mitochondrial

Gene
N/A
Organism
Lithobates catesbeiana (American bullfrog) (Rana catesbeiana)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell.By similarity

    Catalytic activityi

    2 ATP + NH3 + CO2 + H2O = 2 ADP + phosphate + carbamoyl phosphate.

    Enzyme regulationi

    Requires N-acetyl-L-glutamate (NAG) as an allosteric activator.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei290 – 2901For GATase activityBy similarity
    Binding sitei1388 – 13881Allosteric activatorBy similarity
    Binding sitei1391 – 13911Allosteric activatorBy similarity
    Binding sitei1407 – 14071Allosteric activatorBy similarity
    Binding sitei1433 – 14331Allosteric activatorBy similarity
    Binding sitei1436 – 14361Allosteric activatorBy similarity
    Binding sitei1445 – 14451Allosteric activatorBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. carbamoyl-phosphate synthase (ammonia) activity Source: UniProtKB-EC
    3. metal ion binding Source: InterPro

    GO - Biological processi

    1. carbamoyl phosphate biosynthetic process Source: InterPro
    2. glutamine catabolic process Source: InterPro
    3. urea cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Urea cycle

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Carbamoyl-phosphate synthase [ammonia], mitochondrial (EC:6.3.4.16)
    Alternative name(s):
    Carbamoyl-phosphate synthetase I
    Short name:
    CPSase I
    OrganismiLithobates catesbeiana (American bullfrog) (Rana catesbeiana)
    Taxonomic identifieri8400 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraNeobatrachiaRanoideaRanidaeRanaAquarana

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3333MitochondrionBy similarityAdd
    BLAST
    Chaini34 – 14961463Carbamoyl-phosphate synthase [ammonia], mitochondrialPRO_0000029900Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ91293.
    SMRiQ91293. Positions 1340-1474.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini215 – 401187Glutamine amidotransferase type-1Add
    BLAST
    Domaini548 – 740193ATP-grasp 1Add
    BLAST
    Domaini1090 – 1281192ATP-grasp 2Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni34 – 214181Anthranilate phosphoribosyltransferase homologAdd
    BLAST

    Sequence similaritiesi

    Contains 2 ATP-grasp domains.Curated

    Keywords - Domaini

    Repeat, Transit peptide

    Phylogenomic databases

    HOVERGENiHBG000279.

    Family and domain databases

    Gene3Di1.10.1030.10. 1 hit.
    3.30.1490.20. 2 hits.
    3.30.470.20. 2 hits.
    3.40.50.1380. 1 hit.
    3.40.50.20. 2 hits.
    3.40.50.880. 1 hit.
    3.50.30.20. 1 hit.
    HAMAPiMF_01209. CPSase_S_chain.
    InterProiIPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR006275. CarbamoylP_synth_lsu.
    IPR005481. CarbamoylP_synth_lsu_N.
    IPR005480. CarbamoylP_synth_lsu_oligo.
    IPR006274. CarbamoylP_synth_ssu.
    IPR002474. CarbamoylP_synth_ssu_N.
    IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
    IPR005483. CbamoylP_synth_lsu_CPSase_dom.
    IPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    IPR011607. MGS-like_dom.
    IPR016185. PreATP-grasp_dom.
    [Graphical view]
    PfamiPF00289. CPSase_L_chain. 2 hits.
    PF02786. CPSase_L_D2. 2 hits.
    PF02787. CPSase_L_D3. 1 hit.
    PF00988. CPSase_sm_chain. 1 hit.
    PF00117. GATase. 1 hit.
    PF02142. MGS. 1 hit.
    [Graphical view]
    PRINTSiPR00098. CPSASE.
    SMARTiSM01096. CPSase_L_D3. 1 hit.
    SM01097. CPSase_sm_chain. 1 hit.
    SM00851. MGS. 1 hit.
    [Graphical view]
    SUPFAMiSSF48108. SSF48108. 1 hit.
    SSF52021. SSF52021. 1 hit.
    SSF52317. SSF52317. 1 hit.
    SSF52335. SSF52335. 1 hit.
    SSF52440. SSF52440. 2 hits.
    TIGRFAMsiTIGR01369. CPSaseII_lrg. 1 hit.
    TIGR01368. CPSaseIIsmall. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 2 hits.
    PS00866. CPSASE_1. 2 hits.
    PS00867. CPSASE_2. 2 hits.
    PS51273. GATASE_TYPE_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q91293-1 [UniParc]FASTAAdd to Basket

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    MTRILSVFKT AKTGVLNAAA HRYRGFSKAG VRLMSVKAQT ANLVLEDGTK     50
    IKGYSFGHPA SVAGEVIFNT GLGGYVEAVT DPSYHGQILT LTNPIIGNGG 100
    APDTKARDAY GLMKYIESEN IQASGLLVLD YSHEYSHWGA VKSLSEWLHE 150
    EKVPALCGID TRMLAKKIRD NKGAVLGKIE FEGQPVEFID PNKRNLIAEV 200
    STKETKVFGK GNPVRIVAVD CGVKHNIIRQ LVKRGAEVHL VPWNHDFSQM 250
    EYDGLLITSG PGNPELAKPL IQNLKKVFQS DRPEPIFGIC KGNEIAALAA 300
    GGKTYRLPMA NRGQNQPVMI TLNGQAFITA QNHAYAVDNN SLPAGWKPLF 350
    VNINDQSNEG IMHETKPIFT SQFHPEANPG PVDTEFLFDV YMSLIKKGKG 400
    TTLTSVMPKP ALQSKRIDVA KVLILGSGGL SIGQAGEFDY SGSQAVKAMK 450
    EENVKTVLMN PNIASVQTNE VGLKQADTVY FLPITPQFVT EVIKAEKTDG 500
    IILGMGGQTA LNCGVELFKR GVLKEYGVRV LGTSVESIMF TEDRQLFSDK 550
    LNEIKEPIAP SFAVESVKDA LEAADKIGYP VMIRSAYALG GLGSGLCPDK 600
    ETLTDLATKA LAMTNQILVE RSVVGWKEIE YEVVRDAADN CVTVCNMENV 650
    DAMGVHTGDS IVVAPCQTLS NEECQMLRAV SIKVVRHLGI VGECNIQFAL 700
    HPTSLEYVII EVNARLSRSS ALASKATGYP LAFIAAKIAL GIPLPEIKNV 750
    VSGKTTACFE PSLDYMVTKI PRWDLDRFHG ASGLIGSSMK SVGEVMAIGR 800
    TFEESFQKAL RMCHPSVDGF TSNLPMNKAW SSDVNLRKEM AEPTSTRMYS 850
    MAKAIQSGIS LDEINKLTAI DKWFLYKMQG ILNMEKTLKG SRSESVPEET 900
    LRRAKQIGFS DRYIGKCLGL SETQTRELRL NKNVKPWVKQ IDTLAAEYPA 950
    ITNYLYLTYN GQEHDIKFDD HGMMVLGCGP YHIGSSVEFD WCAVSSIRTL 1000
    RHVGKKTVVV NCNPETVSTD FDECDKLYFE ELSQERIMDV FQLEQCDGCI 1050
    ISVGGQIPNN LAVPLYKNGV KIMGTSPMQI DRAEDRSIFS AVLDELQIAQ 1100
    APWKAVNSLD DALQFTKTVG YPCLLRPSYV LSGSAMNVVY GEEELKTFLA 1150
    EATRVSQEHP VVITKFIEGA REVEMDAVGK EGRVISHAIS EHVEDAGVHS 1200
    GDATLMIPTQ SISQGAIEKV KIATKKIATA FAISGPFNVQ FLVRGNDVLV 1250
    IECNLRASRS FPFVSKTLGV DFIDVATKVM IGEKIDESSL PTLERPVIPA 1300
    DYVGIKAPMF SWPRLRGADP VLKCEMASTG EVACFGQNVY SAFLKAMIST 1350
    GFKLPQKGIL IGIQHSFRPH FLGTAQTLKD EGFKLYATEA TADWLNANDI 1400
    TATPVAWPSQ EGQSGPSSIY KLIKEGNIDM VINLPNNNTK YVRDNFAIRR 1450
    TAVDTGTALL TNFQVVKMFA EAIKYSGDLD AKSLFHYRQF GGAKPS 1496
    Length:1,496
    Mass (Da):163,767
    Last modified:November 1, 1996 - v1
    Checksum:i2360BF05D21B059E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U05193 mRNA. Translation: AAA19016.1.
    PIRiI51170.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U05193 mRNA. Translation: AAA19016.1 .
    PIRi I51170.

    3D structure databases

    ProteinModelPortali Q91293.
    SMRi Q91293. Positions 1340-1474.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG000279.

    Family and domain databases

    Gene3Di 1.10.1030.10. 1 hit.
    3.30.1490.20. 2 hits.
    3.30.470.20. 2 hits.
    3.40.50.1380. 1 hit.
    3.40.50.20. 2 hits.
    3.40.50.880. 1 hit.
    3.50.30.20. 1 hit.
    HAMAPi MF_01209. CPSase_S_chain.
    InterProi IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR006275. CarbamoylP_synth_lsu.
    IPR005481. CarbamoylP_synth_lsu_N.
    IPR005480. CarbamoylP_synth_lsu_oligo.
    IPR006274. CarbamoylP_synth_ssu.
    IPR002474. CarbamoylP_synth_ssu_N.
    IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
    IPR005483. CbamoylP_synth_lsu_CPSase_dom.
    IPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    IPR011607. MGS-like_dom.
    IPR016185. PreATP-grasp_dom.
    [Graphical view ]
    Pfami PF00289. CPSase_L_chain. 2 hits.
    PF02786. CPSase_L_D2. 2 hits.
    PF02787. CPSase_L_D3. 1 hit.
    PF00988. CPSase_sm_chain. 1 hit.
    PF00117. GATase. 1 hit.
    PF02142. MGS. 1 hit.
    [Graphical view ]
    PRINTSi PR00098. CPSASE.
    SMARTi SM01096. CPSase_L_D3. 1 hit.
    SM01097. CPSase_sm_chain. 1 hit.
    SM00851. MGS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48108. SSF48108. 1 hit.
    SSF52021. SSF52021. 1 hit.
    SSF52317. SSF52317. 1 hit.
    SSF52335. SSF52335. 1 hit.
    SSF52440. SSF52440. 2 hits.
    TIGRFAMsi TIGR01369. CPSaseII_lrg. 1 hit.
    TIGR01368. CPSaseIIsmall. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 2 hits.
    PS00866. CPSASE_1. 2 hits.
    PS00867. CPSASE_2. 2 hits.
    PS51273. GATASE_TYPE_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "3,5,3'-Triiodothyronine-induced carbamyl-phosphate synthetase gene expression is stabilized in the liver of Rana catesbeiana tadpoles during heat shock."
      Helbing C.C., Atkinson B.G.
      J. Biol. Chem. 269:11743-11750(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.

    Entry informationi

    Entry nameiCPSM_LITCT
    AccessioniPrimary (citable) accession number: Q91293
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3