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Q91293

- CPSM_LITCT

UniProt

Q91293 - CPSM_LITCT

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Protein

Carbamoyl-phosphate synthase [ammonia], mitochondrial

Gene
N/A
Organism
Lithobates catesbeiana (American bullfrog) (Rana catesbeiana)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell By similarity.UniRule annotation

Catalytic activityi

2 ATP + NH3 + CO2 + H2O = 2 ADP + phosphate + carbamoyl phosphate.UniRule annotation

Enzyme regulationi

Requires N-acetyl-L-glutamate (NAG) as an allosteric activator By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei290 – 2901For GATase activity By similarity
Binding sitei1388 – 13881Allosteric activator By similarity
Binding sitei1391 – 13911Allosteric activator By similarity
Binding sitei1407 – 14071Allosteric activator By similarity
Binding sitei1433 – 14331Allosteric activator By similarity
Binding sitei1436 – 14361Allosteric activator By similarity
Binding sitei1445 – 14451Allosteric activator By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. carbamoyl-phosphate synthase (ammonia) activity Source: UniProtKB-EC
  3. metal ion binding Source: InterPro

GO - Biological processi

  1. carbamoyl phosphate biosynthetic process Source: InterPro
  2. glutamine catabolic process Source: InterPro
  3. urea cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Urea cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase [ammonia], mitochondrial (EC:6.3.4.16)
Alternative name(s):
Carbamoyl-phosphate synthetase I
Short name:
CPSase I
OrganismiLithobates catesbeiana (American bullfrog) (Rana catesbeiana)
Taxonomic identifieri8400 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraNeobatrachiaRanoideaRanidaeRanaAquarana

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3333Mitochondrion By similarityAdd
BLAST
Chaini34 – 14961463Carbamoyl-phosphate synthase [ammonia], mitochondrialUniRule annotationPRO_0000029900Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ91293.
SMRiQ91293. Positions 1340-1474.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini215 – 401187Glutamine amidotransferase type-1Add
BLAST
Domaini548 – 740193ATP-grasp 1Add
BLAST
Domaini1090 – 1281192ATP-grasp 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni34 – 214181Anthranilate phosphoribosyltransferase homologUniRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 ATP-grasp domains.

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

HOVERGENiHBG000279.

Family and domain databases

Gene3Di1.10.1030.10. 1 hit.
3.30.1490.20. 2 hits.
3.30.470.20. 2 hits.
3.40.50.1380. 1 hit.
3.40.50.20. 2 hits.
3.40.50.880. 1 hit.
3.50.30.20. 1 hit.
HAMAPiMF_01209. CPSase_S_chain.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006275. CarbamoylP_synth_lsu.
IPR005481. CarbamoylP_synth_lsu_N.
IPR005480. CarbamoylP_synth_lsu_oligo.
IPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR005483. CbamoylP_synth_lsu_CPSase_dom.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR011607. MGS-like_dom.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF00289. CPSase_L_chain. 2 hits.
PF02786. CPSase_L_D2. 2 hits.
PF02787. CPSase_L_D3. 1 hit.
PF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PRINTSiPR00098. CPSASE.
SMARTiSM01096. CPSase_L_D3. 1 hit.
SM01097. CPSase_sm_chain. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMiSSF48108. SSF48108. 1 hit.
SSF52021. SSF52021. 1 hit.
SSF52317. SSF52317. 1 hit.
SSF52335. SSF52335. 1 hit.
SSF52440. SSF52440. 2 hits.
TIGRFAMsiTIGR01369. CPSaseII_lrg. 1 hit.
TIGR01368. CPSaseIIsmall. 1 hit.
PROSITEiPS50975. ATP_GRASP. 2 hits.
PS00866. CPSASE_1. 2 hits.
PS00867. CPSASE_2. 2 hits.
PS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91293-1 [UniParc]FASTAAdd to Basket

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MTRILSVFKT AKTGVLNAAA HRYRGFSKAG VRLMSVKAQT ANLVLEDGTK     50
IKGYSFGHPA SVAGEVIFNT GLGGYVEAVT DPSYHGQILT LTNPIIGNGG 100
APDTKARDAY GLMKYIESEN IQASGLLVLD YSHEYSHWGA VKSLSEWLHE 150
EKVPALCGID TRMLAKKIRD NKGAVLGKIE FEGQPVEFID PNKRNLIAEV 200
STKETKVFGK GNPVRIVAVD CGVKHNIIRQ LVKRGAEVHL VPWNHDFSQM 250
EYDGLLITSG PGNPELAKPL IQNLKKVFQS DRPEPIFGIC KGNEIAALAA 300
GGKTYRLPMA NRGQNQPVMI TLNGQAFITA QNHAYAVDNN SLPAGWKPLF 350
VNINDQSNEG IMHETKPIFT SQFHPEANPG PVDTEFLFDV YMSLIKKGKG 400
TTLTSVMPKP ALQSKRIDVA KVLILGSGGL SIGQAGEFDY SGSQAVKAMK 450
EENVKTVLMN PNIASVQTNE VGLKQADTVY FLPITPQFVT EVIKAEKTDG 500
IILGMGGQTA LNCGVELFKR GVLKEYGVRV LGTSVESIMF TEDRQLFSDK 550
LNEIKEPIAP SFAVESVKDA LEAADKIGYP VMIRSAYALG GLGSGLCPDK 600
ETLTDLATKA LAMTNQILVE RSVVGWKEIE YEVVRDAADN CVTVCNMENV 650
DAMGVHTGDS IVVAPCQTLS NEECQMLRAV SIKVVRHLGI VGECNIQFAL 700
HPTSLEYVII EVNARLSRSS ALASKATGYP LAFIAAKIAL GIPLPEIKNV 750
VSGKTTACFE PSLDYMVTKI PRWDLDRFHG ASGLIGSSMK SVGEVMAIGR 800
TFEESFQKAL RMCHPSVDGF TSNLPMNKAW SSDVNLRKEM AEPTSTRMYS 850
MAKAIQSGIS LDEINKLTAI DKWFLYKMQG ILNMEKTLKG SRSESVPEET 900
LRRAKQIGFS DRYIGKCLGL SETQTRELRL NKNVKPWVKQ IDTLAAEYPA 950
ITNYLYLTYN GQEHDIKFDD HGMMVLGCGP YHIGSSVEFD WCAVSSIRTL 1000
RHVGKKTVVV NCNPETVSTD FDECDKLYFE ELSQERIMDV FQLEQCDGCI 1050
ISVGGQIPNN LAVPLYKNGV KIMGTSPMQI DRAEDRSIFS AVLDELQIAQ 1100
APWKAVNSLD DALQFTKTVG YPCLLRPSYV LSGSAMNVVY GEEELKTFLA 1150
EATRVSQEHP VVITKFIEGA REVEMDAVGK EGRVISHAIS EHVEDAGVHS 1200
GDATLMIPTQ SISQGAIEKV KIATKKIATA FAISGPFNVQ FLVRGNDVLV 1250
IECNLRASRS FPFVSKTLGV DFIDVATKVM IGEKIDESSL PTLERPVIPA 1300
DYVGIKAPMF SWPRLRGADP VLKCEMASTG EVACFGQNVY SAFLKAMIST 1350
GFKLPQKGIL IGIQHSFRPH FLGTAQTLKD EGFKLYATEA TADWLNANDI 1400
TATPVAWPSQ EGQSGPSSIY KLIKEGNIDM VINLPNNNTK YVRDNFAIRR 1450
TAVDTGTALL TNFQVVKMFA EAIKYSGDLD AKSLFHYRQF GGAKPS 1496
Length:1,496
Mass (Da):163,767
Last modified:November 1, 1996 - v1
Checksum:i2360BF05D21B059E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U05193 mRNA. Translation: AAA19016.1.
PIRiI51170.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U05193 mRNA. Translation: AAA19016.1 .
PIRi I51170.

3D structure databases

ProteinModelPortali Q91293.
SMRi Q91293. Positions 1340-1474.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG000279.

Family and domain databases

Gene3Di 1.10.1030.10. 1 hit.
3.30.1490.20. 2 hits.
3.30.470.20. 2 hits.
3.40.50.1380. 1 hit.
3.40.50.20. 2 hits.
3.40.50.880. 1 hit.
3.50.30.20. 1 hit.
HAMAPi MF_01209. CPSase_S_chain.
InterProi IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006275. CarbamoylP_synth_lsu.
IPR005481. CarbamoylP_synth_lsu_N.
IPR005480. CarbamoylP_synth_lsu_oligo.
IPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR005483. CbamoylP_synth_lsu_CPSase_dom.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR011607. MGS-like_dom.
IPR016185. PreATP-grasp_dom.
[Graphical view ]
Pfami PF00289. CPSase_L_chain. 2 hits.
PF02786. CPSase_L_D2. 2 hits.
PF02787. CPSase_L_D3. 1 hit.
PF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view ]
PRINTSi PR00098. CPSASE.
SMARTi SM01096. CPSase_L_D3. 1 hit.
SM01097. CPSase_sm_chain. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view ]
SUPFAMi SSF48108. SSF48108. 1 hit.
SSF52021. SSF52021. 1 hit.
SSF52317. SSF52317. 1 hit.
SSF52335. SSF52335. 1 hit.
SSF52440. SSF52440. 2 hits.
TIGRFAMsi TIGR01369. CPSaseII_lrg. 1 hit.
TIGR01368. CPSaseIIsmall. 1 hit.
PROSITEi PS50975. ATP_GRASP. 2 hits.
PS00866. CPSASE_1. 2 hits.
PS00867. CPSASE_2. 2 hits.
PS51273. GATASE_TYPE_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "3,5,3'-Triiodothyronine-induced carbamyl-phosphate synthetase gene expression is stabilized in the liver of Rana catesbeiana tadpoles during heat shock."
    Helbing C.C., Atkinson B.G.
    J. Biol. Chem. 269:11743-11750(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiCPSM_LITCT
AccessioniPrimary (citable) accession number: Q91293
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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