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Protein

Non-structural protein 5

Gene
N/A
Organism
Rotavirus A (strain RVA/Human/United States/M/1976/G3P[X]) (RV-A)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Plays an essential role in the viral genome replication. Participates, together with NSP2, in the formation of viral factories (viroplasms) which are large inclusions in the host cytoplasm where replication intermediates are assembled and viral RNA replication takes place. Orchestrates the recruitment of viroplasmic proteins such as capsid proteins to these factories.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi92MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
LigandMagnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 5UniRule annotation
Short name:
NSP5UniRule annotation
Alternative name(s):
NS26UniRule annotation
OrganismiRotavirus A (strain RVA/Human/United States/M/1976/G3P[X]) (RV-A)
Taxonomic identifieri578834 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiHomo sapiens (Human) [TaxID: 9606]

Subcellular locationi

  • Host cytoplasm UniRule annotation
  • Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003695061 – 197Non-structural protein 5Add BLAST197

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei67Phosphoserine; by host CK1UniRule annotation1
Modified residuei153Phosphoserine; by hostUniRule annotation1
Modified residuei155Phosphoserine; by hostUniRule annotation1
Modified residuei163Phosphoserine; by hostUniRule annotation1
Modified residuei165Phosphoserine; by hostUniRule annotation1

Post-translational modificationi

O-glycosylated.UniRule annotation
Hyperphosphorylated on serine residues, when in dimeric form. Phosphorylation by host CK1 is required for the hyperphosphorylation of NSP5 dimer.UniRule annotation

Keywords - PTMi

Glycoprotein, Phosphoprotein

Interactioni

Subunit structurei

Homodimer. Interacts with VP1. Interacts with VP2. Interacts with NSP2; this interaction leads to up-regulation of NSP5 hyperphosphorylation and formation of virus factories. Interacts with NSP6.UniRule annotation

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi2 – 101Ser-richAdd BLAST100

Sequence similaritiesi

Belongs to the rotavirus NSP5 family.UniRule annotation

Family and domain databases

HAMAPiMF_04092 ROTA_NSP5, 1 hit
InterProiView protein in InterPro
IPR002512 Rotavirus_A/C_NSP5
PfamiView protein in Pfam
PF01525 Rota_NS26, 1 hit
PIRSFiPIRSF004006 Rota_NS26, 1 hit

Sequencei

Sequence statusi: Complete.

Q910F1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLSIDVTSL PSISSNIFKN ESSSTTSTLS GKSIGRNEQY VSSDIEAFNK
60 70 80 90 100
YMLSKSPEDI GPSDSASNDP LTSFSIRSNA VKTNADAGVS MDSSTQSRPS
110 120 130 140 150
SNVGCDQMDF SLTKGINVSA SLDSCVSIST NHKKEKSKKD KSRKHYPRIE
160 170 180 190
ADSDSEDYVL DDSDSDDGKC KNCKYKKKYF ALRMRMKQVA MQLIEDL
Length:197
Mass (Da):21,622
Last modified:December 1, 2001 - v1
Checksum:iDCB2F4BBD99F6056
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF338244 Genomic RNA Translation: AAK74114.1
AF338245 Genomic RNA Translation: AAK74115.1

Similar proteinsi

Entry informationi

Entry nameiNSP5_ROTAM
AccessioniPrimary (citable) accession number: Q910F1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: December 1, 2001
Last modified: April 25, 2018
This is version 36 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health