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Q91055

- CRVP_HELHO

UniProt

Q91055 - CRVP_HELHO

Protein

Cysteine-rich venom protein helothermine

Gene
N/A
Organism
Heloderma horridum horridum (Mexican beaded lizard)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Alters a variety of ion channel activities, including voltage-gated potassium channels (Kv) (PubMed:8071987), voltage-gated calcium channels (L-, N-, and P-type) (Cav) (PubMed:8817251) and ryanodine receptors (RyR) (PubMed:7647234). Is toxic to mice (causes lethargy, partial paralysis of rear limbs and lowering of body temperature).3 Publications

    Keywords - Molecular functioni

    Calcium channel impairing toxin, Ion channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Ryanodine-sensitive calcium-release channel impairing toxin, Toxin, Voltage-gated calcium channel impairing toxin, Voltage-gated potassium channel impairing toxin

    Protein family/group databases

    TCDBi8.B.9.1.3. the triflin toxin (triflin or crisp) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cysteine-rich venom protein helothermine
    Short name:
    CRVP HLTx
    OrganismiHeloderma horridum horridum (Mexican beaded lizard)
    Taxonomic identifieri8552 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaAnguimorphaNeoanguimorphaHelodermatidaeHeloderma

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 19191 PublicationAdd
    BLAST
    Chaini20 – 242223Cysteine-rich venom protein helotherminePRO_0000006272Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi77 ↔ 155PROSITE-ProRule annotation
    Disulfide bondi94 ↔ 170PROSITE-ProRule annotation
    Disulfide bondi150 ↔ 167PROSITE-ProRule annotation
    Disulfide bondi189 ↔ 196PROSITE-ProRule annotation
    Disulfide bondi192 ↔ 201PROSITE-ProRule annotation
    Disulfide bondi205 ↔ 237PROSITE-ProRule annotation
    Disulfide bondi214 ↔ 231PROSITE-ProRule annotation
    Disulfide bondi223 ↔ 235PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.

    Structurei

    3D structure databases

    ProteinModelPortaliQ91055.
    SMRiQ91055. Positions 34-241.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini41 – 169129SCPAdd
    BLAST
    Domaini205 – 23733ShKTPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the CRISP family.Curated
    Contains 1 SCP domain.Curated
    Contains 1 ShKT domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOVERGENiHBG004184.

    Family and domain databases

    Gene3Di3.40.33.10. 1 hit.
    InterProiIPR001283. Allrgn_V5/Tpx1.
    IPR018244. Allrgn_V5/Tpx1_CS.
    IPR014044. CAP_domain.
    IPR013871. Cysteine_rich_secretory.
    IPR003582. ShKT_dom.
    [Graphical view]
    PANTHERiPTHR10334. PTHR10334. 1 hit.
    PfamiPF00188. CAP. 1 hit.
    PF08562. Crisp. 1 hit.
    [Graphical view]
    PRINTSiPR00837. V5TPXLIKE.
    SMARTiSM00198. SCP. 1 hit.
    [Graphical view]
    SUPFAMiSSF55797. SSF55797. 1 hit.
    PROSITEiPS01009. CRISP_1. 1 hit.
    PS51670. SHKT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q91055-1 [UniParc]FASTAAdd to Basket

    « Hide

    MILLSLYLCL AAMLHQSEGE ASPKLPGLMT SNPDQQTEIT DKHNNLRRIV    50
    EPTASNMLKM TWSNKIAQNA QRSANQCTLE HTSKEERTID GVECGENLFF 100
    SSAPYTWSYA IQNWFDERKY FRFNYGPTAQ NVMIGHYTQV VWYRSYELGC 150
    AIAYCPDQPT YKYYQVCQYC PGGNIRSRKY TPYSIGPPCG DCPDACDNGL 200
    CTNPCKQNDV YNNCPDLKKQ VGCGHPIMKD CMATCKCLTE IK 242
    Length:242
    Mass (Da):27,493
    Last modified:November 1, 1996 - v1
    Checksum:i0E183FC2F925DF3C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U13619 mRNA. Translation: AAC59730.1.
    PIRiA34859.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U13619 mRNA. Translation: AAC59730.1 .
    PIRi A34859.

    3D structure databases

    ProteinModelPortali Q91055.
    SMRi Q91055. Positions 34-241.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    TCDBi 8.B.9.1.3. the triflin toxin (triflin or crisp) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG004184.

    Family and domain databases

    Gene3Di 3.40.33.10. 1 hit.
    InterProi IPR001283. Allrgn_V5/Tpx1.
    IPR018244. Allrgn_V5/Tpx1_CS.
    IPR014044. CAP_domain.
    IPR013871. Cysteine_rich_secretory.
    IPR003582. ShKT_dom.
    [Graphical view ]
    PANTHERi PTHR10334. PTHR10334. 1 hit.
    Pfami PF00188. CAP. 1 hit.
    PF08562. Crisp. 1 hit.
    [Graphical view ]
    PRINTSi PR00837. V5TPXLIKE.
    SMARTi SM00198. SCP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55797. SSF55797. 1 hit.
    PROSITEi PS01009. CRISP_1. 1 hit.
    PS51670. SHKT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure and properties of helothermine, a peptide toxin that blocks ryanodine receptors."
      Morrissette J., Kraetzschmar J., Haendler B., El-Hayek R., Mochca-Morales J., Martin B.M., Patel J.R., Moss R.L., Schleuning W.-D., Coronado R., Possani L.D.
      Biophys. J. 68:2280-2288(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, FUNCTION.
    2. "Isolation and characterization of helothermine, a novel toxin from Heloderma horridum horridum (Mexican beaded lizard) venom."
      Mochca-Morales J., Martin B.M., Possani L.D.
      Toxicon 28:299-309(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 20-39, CHARACTERIZATION.
      Tissue: Venom.
    3. "The toxin helothermine affects potassium currents in newborn rat cerebellar granule cells."
      Nobile M., Magnelli V., Lagostena L., Mochca-Morales J., Possani L.D., Prestipino G.
      J. Membr. Biol. 139:49-55(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "Helothermine, a lizard venom toxin, inhibits calcium current in cerebellar granules."
      Nobile M., Noceti F., Prestipino G., Possani L.D.
      Exp. Brain Res. 110:15-20(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiCRVP_HELHO
    AccessioniPrimary (citable) accession number: Q91055
    Secondary accession number(s): P46693
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programAnimal Toxin Annotation Program
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    IC50=0.52 µM on IA-type current, and 0.86 µM on delayed rectifier current.1 Publication

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3