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Protein

Thrombin-like enzyme calobin-1

Gene
N/A
Organism
Gloydius ussuriensis (Ussuri mamushi) (Gloydius blomhoffii ussuriensis)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Thrombin-like snake venom serine protease. Has a coagulant activity. Acts on alpha-chains of fibrinogen (FGA) generating fibrinopeptide A.1 Publication

Enzyme regulationi

Strongly inhibited by PMSF, and moderately by benzamidine and soybean trypsin inhibitor.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei67 – 671Charge relay systemBy similarity
Active sitei112 – 1121Charge relay systemBy similarity
Active sitei208 – 2081Charge relay systemBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Blood coagulation cascade activating toxin, Hemostasis impairing toxin, Hydrolase, Protease, Serine protease, Toxin

Protein family/group databases

MEROPSiS01.335.

Names & Taxonomyi

Protein namesi
Recommended name:
Thrombin-like enzyme calobin-1 (EC:3.4.21.-)
Short name:
SVTLE
Alternative name(s):
Calobin I
Fibrinogen-clotting enzyme
Snake venom serine protease
Short name:
SVSP
OrganismiGloydius ussuriensis (Ussuri mamushi) (Gloydius blomhoffii ussuriensis)
Taxonomic identifieri35671 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeGloydius

Subcellular locationi

  • Secreted 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818By similarityAdd
BLAST
Propeptidei19 – 246PRO_0000028369
Chaini25 – 262238Thrombin-like enzyme calobin-1PRO_0000028370Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 165PROSITE-ProRule annotation
Disulfide bondi52 ↔ 68PROSITE-ProRule annotation
Disulfide bondi100 ↔ 260PROSITE-ProRule annotation
Glycosylationi105 – 1051N-linked (GlcNAc...)1 Publication
Disulfide bondi144 ↔ 214PROSITE-ProRule annotation
Disulfide bondi176 ↔ 193PROSITE-ProRule annotation
Disulfide bondi204 ↔ 229PROSITE-ProRule annotation

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Expressioni

Tissue specificityi

Expressed by the venom gland.1 Publication

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ91053.
SMRiQ91053. Positions 25-262.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 253229Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Snake venom subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG013304.

Family and domain databases

InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91053-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLISVLANL LILQLSYAQK SSELVIGGDE CNINEHRFLV ALYNSRSRTL
60 70 80 90 100
FCGGTLINQE WVLTAAHCER KNFRIKLGIH SKKVPNEDEQ TRVPKEKFFC
110 120 130 140 150
LSSKNYTLWD KDIMLIRLDS PVSNSEHIAP LSLPSSPPSV GSVCRIMGWG
160 170 180 190 200
RISPTKETYP DVPHCANINL LEYEMCRAPY PEFGLPATSR TLCAGILEGG
210 220 230 240 250
KDTCRGDSGG PLICNGQFQG IASWGDDPCA QPHKPAAYTK VFDHLDWIQS
260
IIAGNTDASC PP
Length:262
Mass (Da):28,908
Last modified:November 1, 1996 - v1
Checksum:i7F318C933006244D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32937 mRNA. Translation: AAC59906.1.
PIRiJC4803.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32937 mRNA. Translation: AAC59906.1.
PIRiJC4803.

3D structure databases

ProteinModelPortaliQ91053.
SMRiQ91053. Positions 25-262.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS01.335.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG013304.

Family and domain databases

InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Purification and molecular cloning of calobin, a thrombin-like enzyme from Agkistrodon caliginosus (Korean viper)."
    Hahn B.S., Yang K.Y., Park E.M., Chang I.M., Kim Y.S.
    J. Biochem. 119:835-843(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GLYCOSYLATION.
    Tissue: Venom gland.

Entry informationi

Entry nameiVSP1_GLOUS
AccessioniPrimary (citable) accession number: Q91053
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: November 1, 1996
Last modified: March 16, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Does not show lysine esterase and caseinolytic activity.1 Publication

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.