Reviewed, UniProtKB/Swiss-Prot Q91041 (TRYX_GADMO)

Names and origin

Protein names	Recommended name:     Trypsin-10     EC=3.4.21.4 Alternative name(s):     Trypsin X
Organism	Gadus morhua (Atlantic cod)
Taxonomic identifier	8049 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Neoteleostei › Acanthomorpha › Paracanthopterygii › Gadiformes › Gadidae › Gadus

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 13	13	Potential
	Propeptide	14 – 19	6	Activation peptide		PRO_0000028311
	Chain	20 – 241	222	Trypsin-10		PRO_0000028312
Regions
	Domain	20 – 239	220	Peptidase S1
Sites
	Active site	59	1	Charge relay system By similarity
	Active site	103	1	Charge relay system By similarity
	Active site	195	1	Charge relay system By similarity
	Site	189	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	26 ↔ 155		By similarity
	Disulfide bond	44 ↔ 60		By similarity
	Disulfide bond	128 ↔ 228		By similarity
	Disulfide bond	135 ↔ 201		By similarity
	Disulfide bond	166 ↔ 180		By similarity
	Disulfide bond	191 ↔ 215		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q91041-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 853D7C26BCAF9DD7	FASTA	241	25,976
	10 20 30 40 50 60 MKSLIFVLLL GAVFAEEDKI VGGYECTRHS QAHQVSLNSG YHFCGGSLVS KDWVVSAAHC 70 80 90 100 110 120 YKSVLRVRLG EHHIRVNEGT EQFISSSSVI RHPNYSSYNI DNDIMLIKLT EPATLNQYVH 130 140 150 160 170 180 AVALPTECAA DATMCTVSGW GNTMSSVDDG DKLQCLNLPI LSHADCANSY PGMITQSMFC 190 200 210 220 230 240 AGYLEGGKDS CQGDSGGPVV CNGVLQGVVS WGYGCAERDN PGVYAKVCVL SGWVRDTMAS Y

References

[1]

"Isolation and characterization of cDNAs from Atlantic cod encoding two different forms of trypsinogen." Gudmundsdottir A., Gudmundsdottir E., Oskarsson S., Bjarnason J.B., Eakin A.E., Craik C.S. Eur. J. Biochem. 217:1091-1097(1993) [PubMed: 8223632] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pyloric caecum.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X76887 mRNA. Translation: CAA54215.1.
PIR	S39048.
3D structure databases
SMR	Q91041. Positions 20-239.
ModBase	Search...
Protein family/group databases
MEROPS	S01.125.
Phylogenomic databases
HOVERGEN	Q91041.
Enzyme and pathway databases
BRENDA	3.4.21.4. 39336.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. IPR009003. Ser/Cys_Pept_Trypsin-like. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRYX_GADMO

Accession

Primary (citable) accession number: Q91041

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 19, 2010
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)