fbxo5-a

Functionⁱ

Regulates progression through early mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Binds to the APC activators cdc20 and fzr1/cdh1 to prevent APC activation. Can also bind directly to the APC to inhibit substrate-binding.By similarity2 Publications

Pathwayⁱ: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	302 – 364	IBR-typeSequence analysisAdd BLAST		63

GO - Molecular functionⁱ

metal ion binding Source: UniProtKB-KW

Complete GO annotation...

GO - Biological processⁱ

cell division Source: UniProtKB-KW
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
Source: UniProtKB
Inferred from direct assayⁱ
- 11389834
protein ubiquitination Source: UniProtKB-UniPathway
regulation of mitotic cell cycle
Source: UniProtKB
Inferred from direct assayⁱ
- 11389834

Complete GO annotation...

Keywordsⁱ

Biological process	Cell cycle, Cell division, Mitosis, Ubl conjugation pathway
Ligand	Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayⁱ	UPA00143.

UniPathwayⁱ

UPA00143.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: F-box only protein 5-A Alternative name(s): Early mitotic inhibitor 1-A
Gene namesⁱ	Name:fbxo5-a Synonyms:emi1-a
Organismⁱ	Xenopus laevis (African clawed frog)
Taxonomic identifierⁱ	8355 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Organism-specific databases

Xenbaseⁱ	XB-GENE-953951. fbxo5.

Xenbaseⁱ

XB-GENE-953951. fbxo5.

Subcellular locationⁱ

Nucleus
Cytoplasm
Cytoplasm › cytoskeleton › spindle

Note:

In interphase, localizes in a punctate manner in the nucleus and cytoplasm with some perinuclear concentration. In mitotic cells, localizes throughout the cell, particularly at the spindle.

GO - Cellular componentⁱ

cytoplasm
Source: UniProtKB
Inferred from direct assayⁱ
- 11389834
nucleus
Source: UniProtKB
Inferred from direct assayⁱ
- 11389834
spindle
Source: UniProtKB
Inferred from direct assayⁱ
- 11389834

Complete GO annotation...

Keywords - Cellular componentⁱ

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	10	S → A: Not mitotically degraded; when associated with A-29; A-105; A-123 and A-328. 1 Publication	1
Mutagenesisⁱ	29	S → A: Not mitotically degraded; when associated with A-10; A-105; A-123 and A-328. 1 Publication	1
Mutagenesisⁱ	105	S → A: Not mitotically degraded; when associated with A-10; A-29; A-123 and A-328. 1 Publication	1
Mutagenesisⁱ	123	T → A: Not mitotically degraded; when associated with A-10; A-29; A-105 and A-328. 1 Publication	1
Mutagenesisⁱ	198 – 199	EL → AA: Abolishes binding to skp1. 1 Publication	2
Mutagenesisⁱ	328	S → A: Not mitotically degraded; when associated with A-10; A-29; A-105 and A-123. 1 Publication	1
Mutagenesisⁱ	341	C → S: Does not inhibit APC. 1 Publication	1
Mutagenesisⁱ	346	C → S: Does not inhibit APC. 1 Publication	1
Mutagenesisⁱ	351	C → S: Does not inhibit APC. 1 Publication	1
Mutagenesisⁱ	354	C → S: Does not inhibit APC; when associated with S-356. 1 Publication	1
Mutagenesisⁱ	356	C → S: Does not inhibit APC; when associated with S-354. 1 Publication	1
Mutagenesisⁱ	364	C → S: Does not inhibit APC. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000258010	1 – 392	F-box only protein 5-AAdd BLAST		392

Expressionⁱ

Developmental stageⁱ

Accumulates before mitosis with levels increasing in S phase and decreasing in M phase.1 Publication

Interactionⁱ

Subunit structureⁱ

Part of a SCF (SKP1-cullin-F-box) protein ligase complex (By similarity). Interacts with btrc, fzr1/cdh1, skp1 and the N-terminal substrate-binding domain of cdc20.Sequence analysisBy similarity1 Publication

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
pin1	Q9I9K6	3	EBI-7161111,EBI-959114
plk1	P70032	2	EBI-7161111,EBI-3511304

Show more details

Protein-protein interaction databases

IntActⁱ	Q90Z80. 3 interactors.
Molecular INTeraction database More...MINTⁱ	MINT-4051752.

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	197 – 244	F-boxSequence analysisAdd BLAST		48

Domainⁱ

The C-terminal region is required for APC inhibition.1 Publication

Zinc finger

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	302 – 364	IBR-typeSequence analysisAdd BLAST		63

Keywords - Domainⁱ

Zinc-finger

Phylogenomic databases

HOVERGENⁱ	HBG010089.
KEGG Orthology (KO) More...KOⁱ	K10292.

Family and domain databases

InterProⁱ	View protein in InterPro IPR001810. F-box_dom.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00646. F-box. 1 hit.
SUPFAMⁱ	SSF81383. SSF81383. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q90Z80-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MMCGFASNQS PKKLSSKKSS ATNVHLEISP VKHHPPCKVY ENVQGSCLDS 
        60         70         80         90        100
AICTTVAKCA DLTDDLPVHN KENLLHRLND LETNSYEEYS ALQDSGYSSI 
       110        120        130        140        150
LQNDSPCQDE TDRKVSDIQV RETPKNFMSY QRPFHTLSKI NLPILRFEEA 
       160        170        180        190        200
VCSTLKKMRK TNKKIDWNAV DVVCGGNYGL EHLIGKSMGL ERFDILAELF 
       210        220        230        240        250
HRDFKHLLTK ILRHLSAMDL INVISVSTTW RKLLQKDNWA YNAYKLGCKE 
       260        270        280        290        300
LCEKRAKVSS HTATRDESLC RVPLASVQKV AASSLCTSKK QSKNKNGGLS 
       310        320        330        340        350
CNRLAEFIEV AQTLKNDQSL KVCVDCGSPA KHDPCLHRAI CTRESCKLDF 
       360        370        380        390 
CTRCSCKYHF SKSCLMSKPG SYRIPSEPLP GSKKSKQNLR RL

Length:392

Mass (Da):44,142

Last modified:December 1, 2001 - v1

Checksum:ⁱ06E6627033263612

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF319594 mRNA. Translation: AAK62272.1. BC088910 mRNA. Translation: AAH88910.1.
NCBI Reference Sequences More...RefSeqⁱ	NP_001082122.1. NM_001088653.1.
UniGeneⁱ	Xl.11644.

Genome annotation databases

GeneIDⁱ	398237.
KEGGⁱ	xla:398237.

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF319594 mRNA. Translation: AAK62272.1. BC088910 mRNA. Translation: AAH88910.1.
NCBI Reference Sequences More...RefSeqⁱ	NP_001082122.1. NM_001088653.1.
UniGeneⁱ	Xl.11644.

3D structure databases

ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

IntActⁱ	Q90Z80. 3 interactors.
Molecular INTeraction database More...MINTⁱ	MINT-4051752.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	398237.
KEGGⁱ	xla:398237.

Organism-specific databases

CTDⁱ	398237.
Xenbaseⁱ	XB-GENE-953951. fbxo5.

Phylogenomic databases

HOVERGENⁱ	HBG010089.
KEGG Orthology (KO) More...KOⁱ	K10292.

Enzyme and pathway databases

UniPathwayⁱ	UPA00143.

UniPathwayⁱ

UPA00143.

Family and domain databases

InterProⁱ	View protein in InterPro IPR001810. F-box_dom.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00646. F-box. 1 hit.
SUPFAMⁱ	SSF81383. SSF81383. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	FBX5A_XENLA
Accessionⁱ	Q90Z80Primary (citable) accession number: Q90Z80
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
	Last sequence update:	December 1, 2001
	Last modified:	June 7, 2017
	This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Documents

PATHWAY comments
Index of metabolic and biosynthesis pathways

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q90Z80 (FBX5A_XENLA)

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F-box only protein 5-A

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein ubiquitination

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Zinc finger

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

Pathwayⁱ: protein ubiquitination

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Family & Domainsⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ