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Protein

F-box only protein 5-A

Gene

fbxo5-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates progression through early mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Binds to the APC activators cdc20 and fzr1/cdh1 to prevent APC activation. Can also bind directly to the APC to inhibit substrate-binding.By similarity2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri302 – 364IBR-typeSequence analysisAdd BLAST63

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Mitosis, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
F-box only protein 5-A
Alternative name(s):
Early mitotic inhibitor 1-A
Gene namesi
Name:fbxo5-a
Synonyms:emi1-a
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-953951. fbxo5.

Subcellular locationi

  • Nucleus
  • Cytoplasm
  • Cytoplasmcytoskeletonspindle

  • Note: In interphase, localizes in a punctate manner in the nucleus and cytoplasm with some perinuclear concentration. In mitotic cells, localizes throughout the cell, particularly at the spindle.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
  • spindle Source: UniProtKB

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi10S → A: Not mitotically degraded; when associated with A-29; A-105; A-123 and A-328. 1 Publication1
Mutagenesisi29S → A: Not mitotically degraded; when associated with A-10; A-105; A-123 and A-328. 1 Publication1
Mutagenesisi105S → A: Not mitotically degraded; when associated with A-10; A-29; A-123 and A-328. 1 Publication1
Mutagenesisi123T → A: Not mitotically degraded; when associated with A-10; A-29; A-105 and A-328. 1 Publication1
Mutagenesisi198 – 199EL → AA: Abolishes binding to skp1. 1 Publication2
Mutagenesisi328S → A: Not mitotically degraded; when associated with A-10; A-29; A-105 and A-123. 1 Publication1
Mutagenesisi341C → S: Does not inhibit APC. 1 Publication1
Mutagenesisi346C → S: Does not inhibit APC. 1 Publication1
Mutagenesisi351C → S: Does not inhibit APC. 1 Publication1
Mutagenesisi354C → S: Does not inhibit APC; when associated with S-356. 1 Publication1
Mutagenesisi356C → S: Does not inhibit APC; when associated with S-354. 1 Publication1
Mutagenesisi364C → S: Does not inhibit APC. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002580101 – 392F-box only protein 5-AAdd BLAST392

Expressioni

Developmental stagei

Accumulates before mitosis with levels increasing in S phase and decreasing in M phase.1 Publication

Interactioni

Subunit structurei

Part of a SCF (SKP1-cullin-F-box) protein ligase complex (By similarity). Interacts with btrc, fzr1/cdh1, skp1 and the N-terminal substrate-binding domain of cdc20.Sequence analysisBy similarity1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

IntActiQ90Z80. 3 interactors.
MINTiMINT-4051752.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini197 – 244F-boxSequence analysisAdd BLAST48

Domaini

The C-terminal region is required for APC inhibition.1 Publication

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri302 – 364IBR-typeSequence analysisAdd BLAST63

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOVERGENiHBG010089.
KOiK10292.

Family and domain databases

InterProiView protein in InterPro
IPR001810. F-box_dom.
PfamiView protein in Pfam
PF00646. F-box. 1 hit.
SUPFAMiSSF81383. SSF81383. 1 hit.

Sequencei

Sequence statusi: Complete.

Q90Z80-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMCGFASNQS PKKLSSKKSS ATNVHLEISP VKHHPPCKVY ENVQGSCLDS
60 70 80 90 100
AICTTVAKCA DLTDDLPVHN KENLLHRLND LETNSYEEYS ALQDSGYSSI
110 120 130 140 150
LQNDSPCQDE TDRKVSDIQV RETPKNFMSY QRPFHTLSKI NLPILRFEEA
160 170 180 190 200
VCSTLKKMRK TNKKIDWNAV DVVCGGNYGL EHLIGKSMGL ERFDILAELF
210 220 230 240 250
HRDFKHLLTK ILRHLSAMDL INVISVSTTW RKLLQKDNWA YNAYKLGCKE
260 270 280 290 300
LCEKRAKVSS HTATRDESLC RVPLASVQKV AASSLCTSKK QSKNKNGGLS
310 320 330 340 350
CNRLAEFIEV AQTLKNDQSL KVCVDCGSPA KHDPCLHRAI CTRESCKLDF
360 370 380 390
CTRCSCKYHF SKSCLMSKPG SYRIPSEPLP GSKKSKQNLR RL
Length:392
Mass (Da):44,142
Last modified:December 1, 2001 - v1
Checksum:i06E6627033263612
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF319594 mRNA. Translation: AAK62272.1.
BC088910 mRNA. Translation: AAH88910.1.
RefSeqiNP_001082122.1. NM_001088653.1.
UniGeneiXl.11644.

Genome annotation databases

GeneIDi398237.
KEGGixla:398237.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiFBX5A_XENLA
AccessioniPrimary (citable) accession number: Q90Z80
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: December 1, 2001
Last modified: June 7, 2017
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways