Q90YK5 (HPSE_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heparanase
EC=3.2.-.-

Gene names

Name:	HPSE
Synonyms:	HPA

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	523 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling By similarity. Increases cell adhesion to the extacellular matrix (ECM), independent of its enzymatic activity. Ref.2
Subunit structure	Heterodimer; the active enzyme is a heterodimer of the 60 kDa and 45 kDa proteolytic products. Ref.1
Subcellular location	Secreted. Note: Localized close to cell surface. Ref.1
Developmental stage	Expressed, as early as 12 h post fertilization, in cells migrating from the epiblast and forming the hypoblast layer. Later on at 72 h, preferentially expressed in cells of the developing vascular and nervous systems. Ref.1
Post-translational modification	N-glycosylated By similarity. Proteolytically cleaved to produce a 60 kDa and a 45 kDa product.
Sequence similarities	Belongs to the glycosyl hydrolase 79 family.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell-matrix adhesion Inferred from direct assay Ref.2. Source: UniProtKB
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, acting on glycosyl bonds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 523	505	Heparanase		PRO_0000042259
Regions
	Region	137 – 141	5	Heparin/HS-binding By similarity
	Region	250 – 260	11	Heparin/HS-binding By similarity
Sites
	Active site	204	1	Proton donor Potential
	Active site	323	1	Nucleophile Potential
Amino acid modifications
	Glycosylation	141	1	N-linked (GlcNAc...) Potential
	Glycosylation	196	1	N-linked (GlcNAc...) Potential
	Glycosylation	436	1	N-linked (GlcNAc...) Potential
	Glycosylation	439	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q90YK5 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 8EB0B7B18C9BF881	FASTA	523	58,386
	10 20 30 40 50 60 MLVLLLLVLL LAVPPRRTAE LQLGLREPIG AVSPAFLSLT LDASLARDPR FVALLRHPKL 70 80 90 100 110 120 HTLASGLSPG FLRFGGTSTD FLIFNPNKDS TWEEKVLSEF QAKDVCEAWP SFAVVPKLLL 130 140 150 160 170 180 TQWPLQEKLL LAEHSWKKHK NTTITRSTLD ILHTFASSSG FRLVFGLNAL LRRAGLQWDS 190 200 210 220 230 240 SNAKQLLGYC AQRSYNISWE LGNEPNSFRK KSGICIDGFQ LGRDFVHLRQ LLSQHPLYRH 250 260 270 280 290 300 AELYGLDVGQ PRKHTQHLLR SFMKSGGKAI DSVTWHHYYV NGRSATREDF LSPEVLDSFA 310 320 330 340 350 360 TAIHDVLGIV EATVPGKKVW LGETGSAYGG GAPQLSNTYV AGFMWLDKLG LAARRGIDVV 370 380 390 400 410 420 MRQVSFGAGS YHLVDAGFKP LPDYWLSLLY KRLVGTRVLQ ASVEQADARR PRVYLHCTNP 430 440 450 460 470 480 RHPKYREGDV TLFALNLSNV TQSLQLPKQL WSKSVDQYLL LPHGKDSILS REVQLNGRLL 490 500 510 520 QMVDDETLPA LHEMALAPGS TLGLPAFSYG FYVIRNAKAI ACI

References

[1]

"Expression pattern and secretion of human and chicken heparanase are determined by their signal peptide sequence." Goldshmidt O., Zcharia E., Aingorn H., Guatta-Rangini Z., Atzmon R., Michal I., Pecker I., Mitrani E., Vlodavsky I. J. Biol. Chem. 276:29178-29187(2001) [PubMed: 11387326] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, SUBUNIT, ENZYME ACTIVITY, DEVELOPMENTAL STAGE.

[2]

"Heparanase mediates cell adhesion independent of its enzymatic activity." Goldshmidt O., Zcharia E., Cohen M., Aingorn H., Cohen I., Nadav L., Katz B.Z., Geiger B., Vlodavsky I. FASEB J. 17:1015-1025(2003) [PubMed: 12773484] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY037007 mRNA. Translation: AAK82648.1.
IPI	IPI00576082.
RefSeq	NP_989498.1. NM_204167.1.
UniGene	Gga.950.
3D structure databases
ProteinModelPortal	Q90YK5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q90YK5.
Protein family/group databases
CAZy	GH79. Glycoside Hydrolase Family 79.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	373981.
KEGG	gga:373981.
Organism-specific databases
CTD	10855.
Phylogenomic databases
eggNOG	veNOG10976.
GeneTree	ENSGT00390000004874.
HOGENOM	HBG715522.
HOVERGEN	HBG081606.
InParanoid	Q90YK5.
OrthoDB	EOG45MN5C.
Family and domain databases
InterPro	IPR005199. Glyco_hydro_79. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
KO	K07964.
PANTHER	PTHR14363. Glyco_hydro_79_N. 1 hit.
Pfam	PF03662. Glyco_hydro_79n. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HPSE_CHICK

Accession

Primary (citable) accession number: Q90YK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2005
Last sequence update:	December 1, 2001
Last modified:	November 16, 2011
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program