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Protein

Heparanase

Gene

HPSE

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety (By similarity). Increases cell adhesion to the extacellular matrix (ECM), independent of its enzymatic activity.By similarity1 Publication

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glycosidic bonds of heparan sulfate chains in heparan sulfate proteoglycan.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei204Proton donorSequence analysis1
Active sitei323NucleophileSequence analysis1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cell adhesion

Protein family/group databases

CAZyiGH79. Glycoside Hydrolase Family 79.

Names & Taxonomyi

Protein namesi
Recommended name:
Heparanase (EC:3.2.1.166)
Gene namesi
Name:HPSE
Synonyms:HPA
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000004225919 – 523HeparanaseAdd BLAST505

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi141N-linked (GlcNAc...)Sequence analysis1
Glycosylationi196N-linked (GlcNAc...)Sequence analysis1
Glycosylationi436N-linked (GlcNAc...)Sequence analysis1
Glycosylationi439N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated.By similarity
Proteolytically cleaved to produce a 60 kDa and a 45 kDa product.

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ90YK5.
PRIDEiQ90YK5.

Expressioni

Developmental stagei

Expressed, as early as 12 h post fertilization, in cells migrating from the epiblast and forming the hypoblast layer. Later on at 72 h, preferentially expressed in cells of the developing vascular and nervous systems.1 Publication

Interactioni

Subunit structurei

Heterodimer; the active enzyme is a heterodimer of the 60 kDa and 45 kDa proteolytic products.1 Publication

Protein-protein interaction databases

STRINGi9031.ENSGALP00000018245.

Structurei

3D structure databases

ProteinModelPortaliQ90YK5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni137 – 141Heparin/HS-bindingBy similarity5
Regioni250 – 260Heparin/HS-bindingBy similarityAdd BLAST11

Sequence similaritiesi

Belongs to the glycosyl hydrolase 79 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IHNV. Eukaryota.
ENOG410YDJW. LUCA.
HOGENOMiHOG000007256.
HOVERGENiHBG081606.
InParanoidiQ90YK5.
KOiK07964.
PhylomeDBiQ90YK5.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR005199. Glyco_hydro_79.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR14363. PTHR14363. 2 hits.
PfamiPF03662. Glyco_hydro_79n. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q90YK5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVLLLLVLL LAVPPRRTAE LQLGLREPIG AVSPAFLSLT LDASLARDPR
60 70 80 90 100
FVALLRHPKL HTLASGLSPG FLRFGGTSTD FLIFNPNKDS TWEEKVLSEF
110 120 130 140 150
QAKDVCEAWP SFAVVPKLLL TQWPLQEKLL LAEHSWKKHK NTTITRSTLD
160 170 180 190 200
ILHTFASSSG FRLVFGLNAL LRRAGLQWDS SNAKQLLGYC AQRSYNISWE
210 220 230 240 250
LGNEPNSFRK KSGICIDGFQ LGRDFVHLRQ LLSQHPLYRH AELYGLDVGQ
260 270 280 290 300
PRKHTQHLLR SFMKSGGKAI DSVTWHHYYV NGRSATREDF LSPEVLDSFA
310 320 330 340 350
TAIHDVLGIV EATVPGKKVW LGETGSAYGG GAPQLSNTYV AGFMWLDKLG
360 370 380 390 400
LAARRGIDVV MRQVSFGAGS YHLVDAGFKP LPDYWLSLLY KRLVGTRVLQ
410 420 430 440 450
ASVEQADARR PRVYLHCTNP RHPKYREGDV TLFALNLSNV TQSLQLPKQL
460 470 480 490 500
WSKSVDQYLL LPHGKDSILS REVQLNGRLL QMVDDETLPA LHEMALAPGS
510 520
TLGLPAFSYG FYVIRNAKAI ACI
Length:523
Mass (Da):58,386
Last modified:December 1, 2001 - v1
Checksum:i8EB0B7B18C9BF881
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY037007 mRNA. Translation: AAK82648.1.
RefSeqiNP_989498.1. NM_204167.1.
UniGeneiGga.950.

Genome annotation databases

GeneIDi373981.
KEGGigga:373981.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY037007 mRNA. Translation: AAK82648.1.
RefSeqiNP_989498.1. NM_204167.1.
UniGeneiGga.950.

3D structure databases

ProteinModelPortaliQ90YK5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000018245.

Protein family/group databases

CAZyiGH79. Glycoside Hydrolase Family 79.

Proteomic databases

PaxDbiQ90YK5.
PRIDEiQ90YK5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi373981.
KEGGigga:373981.

Organism-specific databases

CTDi10855.

Phylogenomic databases

eggNOGiENOG410IHNV. Eukaryota.
ENOG410YDJW. LUCA.
HOGENOMiHOG000007256.
HOVERGENiHBG081606.
InParanoidiQ90YK5.
KOiK07964.
PhylomeDBiQ90YK5.

Miscellaneous databases

PROiQ90YK5.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR005199. Glyco_hydro_79.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR14363. PTHR14363. 2 hits.
PfamiPF03662. Glyco_hydro_79n. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiHPSE_CHICK
AccessioniPrimary (citable) accession number: Q90YK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.