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Q90YK3 (GGLO_SCYTO) Reviewed, UniProtKB/Swiss-Prot

Last modified March 6, 2013. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
L-gulonolactone oxidase
Short name=LGO
EC=1.1.3.8
Alternative name(s):
L-gulono-gamma-lactone oxidase
Short name=GLO
Gene names
Name:GULO
OrganismScyliorhinus torazame (Cloudy catshark)
Taxonomic identifier75743 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Chondrichthyes › Elasmobranchii › Galeomorphii › Galeoidea › Carcharhiniformes › Scyliorhinidae › Scyliorhinus

Protein attributes

Sequence length440 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Oxidizes L-gulono-1,4-lactone to hydrogen peroxide and L-xylo-hexulonolactone which spontaneously isomerizes to L-ascorbate By similarity.

Catalytic activity

L-gulono-1,4-lactone + O2 = L-ascorbate + H2O2.

Cofactor

FAD By similarity.

Pathway

Cofactor biosynthesis; L-ascorbate biosynthesis via UDP-alpha-D-glucuronate pathway; L-ascorbate from UDP-alpha-D-glucuronate: step 4/4.

Subcellular location

Microsome membrane; Single-pass membrane protein By similarity. Endoplasmic reticulum membrane; Single-pass membrane protein By similarity.

Sequence similarities

Belongs to the oxygen-dependent FAD-linked oxidoreductase family.

Contains 1 FAD-binding PCMH-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 440440L-gulonolactone oxidase
PRO_0000128162

Regions

Transmembrane245 – 26723Helical; Potential
Domain17 – 187171FAD-binding PCMH-type

Amino acid modifications

Modified residue541Pros-8alpha-FAD histidine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q90YK3 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: F32FBBE69ACB03B7

FASTA44050,958
        10         20         30         40         50         60 
MDQGTMGYQF ENWATTYSCE PELYFEPTTV EEIRQILELA NQRNKRVKVV GCGHSPSDIA 

        70         80         90        100        110        120 
CTDNYLVRLN KLNRILQVDK ERKWITAEAG ILLSDLNEKL DALGLALSNI GAVSDVALGG 

       130        140        150        160        170        180 
VIGTGTHNTG IQHGILATQI VAMTLMTAAG DTLECSNTVN REIFQATRLH LGSLGVVLNV 

       190        200        210        220        230        240 
TIQCVPAFRI HLQQFPKTLT EVLGDLDTHL KQSEYFRFFW FPHTDKVTVF YADRTNKPIK 

       250        260        270        280        290        300 
TTSSWFWNYA IGYYLLEFLL WISVFVPRLV PWINRLFYWL LYSAKAEQVK RSDKAFNFDC 

       310        320        330        340        350        360 
LFKQHVSDWA LPIKQTRAAL EQLKDWLDNN PNVRAHFPVE VRFVRADDIL LSPCYRQDSC 

       370        380        390        400        410        420 
YINIIMYRPY GKEVPREGYW AMYEEIMKRN GGRPHWAKAH SLLRQDFEKI YPAFHKFCSI 

       430        440 
REELDPSGMF LNNYLEKTFF

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References

[1]"Isolation and transient expression of a cDNA encoding L-gulono-g-lactone oxidase, a key enzyme for L-ascorbic acid biosynthesis, from the tiger shark Scyliorhinus torazame."
Nam Y.K., Cho Y.S., Douglas S.E., Gallant J.W., Reith M.E., Kim D.S.
Aquaculture 209:271-284(2002) [AGRICOLA] [Europe PMC]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY039838 mRNA. Translation: AAK73281.1.

3D structure databases

ProteinModelPortalQ90YK3.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG005834.

Enzyme and pathway databases

UniPathwayUPA00991; UER00939.

Family and domain databases

Gene3D3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
InterProIPR007173. ALO.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR010031. FAD_lactone_oxidase.
IPR023595. LGO_GLO.
IPR006094. Oxid_FAD_bind_N.
IPR006093. Oxy_OxRdtase_FAD_BS.
[Graphical view]
PfamPF04030. ALO. 1 hit.
PF01565. FAD_binding_4. 1 hit.
[Graphical view]
PIRSFPIRSF000136. LGO_GLO. 1 hit.
SUPFAMSSF56176. FAD-binding_2. 1 hit.
TIGRFAMsTIGR01678. FAD_lactone_ox. 1 hit.
PROSITEPS51387. FAD_PCMH. 1 hit.
PS00862. OX2_COVAL_FAD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGGLO_SCYTO
AccessionPrimary (citable) accession number: Q90YK3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: December 1, 2001
Last modified: March 6, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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