Reviewed, UniProtKB/Swiss-Prot Q90YK3 (GGLO_SCYTO)

Names and origin

Protein names

Recommended name:
    L-gulonolactone oxidase
      Short name=LGO
    EC=1.1.3.8
Alternative name(s):
    L-gulono-gamma-lactone oxidase
      Short name=GLO

Gene names

Name:

GULO

Organism

Scyliorhinus torazame (Cloudy catshark)

Taxonomic identifier

75743 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Chondrichthyes › Elasmobranchii › Galeomorphii › Galeoidea › Carcharhiniformes › Scyliorhinidae › Scyliorhinus

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Oxidizes L-gulono-1,4-lactone to hydrogen peroxide and L-xylo-hexulonolactone which spontaneously isomerizes to L-ascorbate By similarity.
Catalytic activity	L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2. L-xylo-hex-2-ulono-1,4-lactone = L-ascorbate.
Cofactor	FAD By similarity.
Pathway	Cofactor biosynthesis; L-ascorbic acid biosynthesis.
Subcellular location	Microsome membrane; Single-pass membrane protein By similarity. Endoplasmic reticulum membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the oxygen-dependent FAD-linked oxidoreductase family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Ascorbate biosynthesis
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	L-ascorbic acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	D-arabinono-1,4-lactone oxidase activity Inferred from electronic annotation. Source: InterPro FAD binding Inferred from electronic annotation. Source: InterPro L-gulonolactone oxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 440	440	L-gulonolactone oxidase		PRO_0000128162
Regions
	Transmembrane	245 – 267	23	Potential
	Domain	17 – 187	171	FAD-binding PCMH-type
Amino acid modifications
	Modified residue	54	1	Tele-8alpha-FAD histidine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q90YK3-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: F32FBBE69ACB03B7	FASTA	440	50,958
	10 20 30 40 50 60 MDQGTMGYQF ENWATTYSCE PELYFEPTTV EEIRQILELA NQRNKRVKVV GCGHSPSDIA 70 80 90 100 110 120 CTDNYLVRLN KLNRILQVDK ERKWITAEAG ILLSDLNEKL DALGLALSNI GAVSDVALGG 130 140 150 160 170 180 VIGTGTHNTG IQHGILATQI VAMTLMTAAG DTLECSNTVN REIFQATRLH LGSLGVVLNV 190 200 210 220 230 240 TIQCVPAFRI HLQQFPKTLT EVLGDLDTHL KQSEYFRFFW FPHTDKVTVF YADRTNKPIK 250 260 270 280 290 300 TTSSWFWNYA IGYYLLEFLL WISVFVPRLV PWINRLFYWL LYSAKAEQVK RSDKAFNFDC 310 320 330 340 350 360 LFKQHVSDWA LPIKQTRAAL EQLKDWLDNN PNVRAHFPVE VRFVRADDIL LSPCYRQDSC 370 380 390 400 410 420 YINIIMYRPY GKEVPREGYW AMYEEIMKRN GGRPHWAKAH SLLRQDFEKI YPAFHKFCSI 430 440 REELDPSGMF LNNYLEKTFF

References

[1]

"Isolation and transient expression of a cDNA encoding L-gulono-g-lactone oxidase, a key enzyme for L-ascorbic acid biosynthesis, from the tiger shark Scyliorhinus torazame." Nam Y.K., Cho Y.S., Douglas S.E., Gallant J.W., Reith M.E., Kim D.S. Aquaculture 209:271-284(2002) [Agricola: IND23320045] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY039838 mRNA. Translation: AAK73281.1.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q90YK3.
Enzyme and pathway databases
BRENDA	1.1.3.8. 266735.
Family and domain databases
InterPro	IPR007173. ALO. IPR016166. FAD-bd_2. IPR016168. FAD-linked_Oxase_FAD-bd_sub2. IPR010031. FAD_lactone_oxidase. IPR006094. Oxid_FAD_bind_N. IPR006093. Oxy_OxRdtase_FAD_BS. [Graphical view]
Gene3D	G3DSA:3.30.465.20. FAD-linked_oxidase_FAD-bd_sub2. 1 hit.
Pfam	PF04030. ALO. 1 hit. PF01565. FAD_binding_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR01678. FAD_lactone_ox. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. PS00862. OX2_COVAL_FAD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GGLO_SCYTO

Accession

Primary (citable) accession number: Q90YK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 31, 2002
Last sequence update:	December 1, 2001
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)