Reviewed, UniProtKB/Swiss-Prot Q90YJ9 (CLAT_CHICK)

Names and origin

Protein names

Recommended name:
    Choline O-acetyltransferase
      Short name=Choline acetylase
      Short name=CHOACTase
      Short name=ChAT
    EC=2.3.1.6

Gene names

Name:

CHAT

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	640 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses By similarity.
Catalytic activity	Acetyl-CoA + choline = CoA + O-acetylcholine.
Tissue specificity	Detected in brain and in embryonic retina. Ref.1
Sequence similarities	Belongs to the carnitine/choline acetyltransferase family.

Ontologies

Keywords
Biological process	Neurotransmitter biosynthesis
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	neurotransmitter biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	choline O-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 640	640	Choline O-acetyltransferase		PRO_0000351549
Regions
	Region	412 – 424	13	Coenzyme A binding By similarity
Sites
	Active site	334	1	Proton acceptor By similarity
	Binding site	450	1	Coenzyme A By similarity
	Binding site	551	1	Coenzyme A By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q90YJ9-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 5D07FB959F4020E4	FASTA	640	72,623
	10 20 30 40 50 60 MPDLEKDMQK KEKDSRSKDE PAVPKLPVPP LQQTLQMYLQ CMKHLVPEEQ FRKTKSIVEQ 70 80 90 100 110 120 FGVAGGLGES LQLILEERRE ETTNWVFNYW LDDMYLNNRL ALPVNSSPAI IFARQNFKDV 130 140 150 160 170 180 NDQLRFAANL ISGVQDYKAL LDSHALPVDF ARGQLSGQPL CMKQYYGLFS SYRLPGHTKD 190 200 210 220 230 240 TLVAQKSCVM PEPEHIIVAC NNQLFVLDVG INFRRLSEGD LFTQLRKIAK MAENEEEMLP 250 260 270 280 290 300 PIGLLTTDGR TEWAEARTIL MKDSTNRDSL DMIERCICLV CLDGTSGVEL NDTNMALQLL 310 320 330 340 350 360 HGGGYHKNGA NRWYDKPMQF VVGRDGVCGT VCEHSPFDGI VLVQCTEHLL KHMKESSKKL 370 380 390 400 410 420 LRADSVSELP APRRLRWKCS PEIQAHLASS AEKLQRIVKN LDFIAYKFVN YGKEFIKKQK 430 440 450 460 470 480 TSPDAYIQVA LQLAFYRCHR RLVPTYESAS IRRFDEGRVD NIRSATAEAF AFVKAMIDDK 490 500 510 520 530 540 PALSDSEKMQ RFKDAIAAQT NYTILAITGM AIDNHLLGLR EVAREHFKEL PEIFTDETYL 550 560 570 580 590 600 TSNRFILSTS QVPTPMEMFC CYGPVVPNGY GACYNPQPEH ILFCISSFKD CKETSSDMLA 610 620 630 640 KAVEESLLEI RDLCNKCSSS TAKSLAKQEE ATQLRSDRKL

References

[1]	"Cloning of chicken choline acetyltransferase and its expression in early embryonic retina." Mukherjee R.S., Hausman R.E. Brain Res. Mol. Brain Res. 129:54-66(2004) [PubMed: 15469882] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Brain.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY044155 mRNA. Translation: AAK94673.1.
IPI	IPI00593659.
RefSeq	NP_989941.1.
UniGene	Gga.1849
3D structure databases
HSSP	HSSP built from PDB template 1NM8 based on UniProtKB P43155.
SMR	Q90YJ9. Positions 19-615.
ModBase	Search...
Protein-protein interaction databases
STRING	Q90YJ9.
Genome annotation databases
Ensembl	ENSGALT00000003601; ENSGALP00000003594; ENSGALG00000002289; Gallus gallus. [Genome view]
GeneID	395314.
KEGG	gga:395314.
Organism-specific databases
CTD	395314.
Phylogenomic databases
HOVERGEN	Q90YJ9.
Family and domain databases
InterPro	IPR000542. Carn_acyl_trans. [Graphical view]
PANTHER	PTHR22589. Carn_acyl_trans. 1 hit.
Pfam	PF00755. Carn_acyltransf. 1 hit. [Graphical view]
PROSITE	PS00439. ACYLTRANSF_C_1. False negative. PS00440. ACYLTRANSF_C_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLAT_CHICK

Accession

Primary (citable) accession number: Q90YJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	December 1, 2001
Last modified:	September 1, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)