Reviewed, UniProtKB/Swiss-Prot Q90YI3 (SYMM_TAKRU)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase, mitochondrial
    EC=6.1.1.10
Alternative name(s):
    Mitochondrial methionine--tRNA ligase
      Short name=MtMetRS
    Methionine--tRNA ligase 2

Gene names

Name:

mars2

Organism

Takifugu rubripes (Japanese pufferfish) (Fugu rubripes)

Taxonomic identifier

31033 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Neoteleostei › Acanthomorpha › Acanthopterygii › Percomorpha › Tetraodontiformes › Tetradontoidea › Tetraodontidae › Takifugu

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 26	26	Mitochondrion Potential
	Chain	27 – 590	564	Methionyl-tRNA synthetase, mitochondrial		PRO_0000045495
Regions
	Motif	51 – 61	11	"HIGH" region
	Motif	342 – 346	5	"KMSKS" region
Sites
	Binding site	345	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q90YI3-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 6DF12F9213457F1D	FASTA	590	67,664
	10 20 30 40 50 60 MRTRFLFLTS GCKAVPELHK IVLANAAPVK NPEIRYASTE NRNYYITTPI FYVNASPHLG 70 80 90 100 110 120 HLYSAVIADC FHRHKQLQGF NSRFATGTDE HGLKIQQAAE AAGKEPLEFC TTVSERFRHL 130 140 150 160 170 180 FSSCNISNTD YIRTTEQRHH RAVQHFWSVL CSKGLIYKGS YEGWYSTQDE SFLTPSQVTT 190 200 210 220 230 240 ALDSTGKEIK VSLESGHKVE WMKEENYMFR LSGFRSQLLD WLRENPRAIQ PERFHHSVLQ 250 260 270 280 290 300 WLQEELPDLS VSRQKSRLQW GIPVPEDDGQ TIYVWLDALV NYLTVVGYPN NHERWWNVAH 310 320 330 340 350 360 HIIGKDILKF HAIYWPSFLL GAGLPLPQTI HVHSHWTVAG KKMSKSLGNV IDPQERSQLF 370 380 390 400 410 420 TTDGLRYFLL RQGVPDTDCD YRDDKVVKLL NAELADALGG LLNRCTATAL NPAQVYASFC 430 440 450 460 470 480 PDSFPRERGG RADDDDYRML ESVRHLPAVV EQHFEDMHVY KALEAVSTCV RQTNGFVQRH 490 500 510 520 530 540 APWKLDRKDS ADQRWLGTII HVSLECLRIY GTLLQPVVPE ISNKLLSRLG VQPHERSWTN 550 560 570 580 590 LNFLPRFEGK DCPFEGRALG SDSGVLFSRL ESQRADQQKN RKMEKGSNLK

References

[1]	"Three way comparative genomic analysis of the BTK locus between man, mouse and Fugu." Goode D., Elgar G. Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ290422 Genomic DNA. Translation: CAC44631.1.
3D structure databases
HSSP	HSSP built from PDB template 1A8H based on UniProtKB P23395.
ModBase	Search...
Protein-protein interaction databases
STRING	Q90YI3.
Genome annotation databases
Ensembl	ENSTRUT00000032083; ENSTRUP00000031960; ENSTRUG00000012627; Takifugu rubripes. [Genome view]
Phylogenomic databases
HOVERGEN	Q90YI3.
OMA	KMSKYAD.
Enzyme and pathway databases
BRENDA	6.1.1.10. 281122.
Family and domain databases
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYMM_TAKRU

Accession

Primary (citable) accession number: Q90YI3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	December 1, 2001
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)