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Reviewed, UniProtKB/Swiss-Prot Q90YB1 (DNLI4_CHICK)

Last modified June 16, 2009. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    DNA ligase 4
    EC=6.5.1.1
Alternative name(s):
    DNA ligase IV
    Polydeoxyribonucleotide synthase [ATP] 4
Gene names
Name: LIG4
ORF Names: RCJMB04_10b2
OrganismGallus gallus (Chicken)
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

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Protein attributes

Sequence length912 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination By similarity.

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Cofactor

Magnesium By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Contains 2 BRCT domains.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 912912DNA ligase 4
PRO_0000059579

Regions

Domain659 – 74890BRCT 1
Domain809 – 912104BRCT 2

Sites

Active site2781N6-AMP-lysine intermediate By similarity
Metal binding3361Magnesium 1 Potential
Metal binding4321Magnesium 2 Potential
Binding site2761ATP By similarity
Binding site2831ATP By similarity
Binding site2981ATP By similarity
Binding site4371ATP By similarity
Binding site4481ATP By similarity
Binding site4541ATP By similarity

Experimental info

Sequence conflict4921V → I in BAB68506. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Q90YB1-1 [UniParc].

Last modified February 7, 2006. Version 2.
Checksum: CBF2899CA76D212D

FASTA912104,450
        10         20         30         40         50         60 
MASAPVLQPS PKRTVASHVP FADLCSTLER IQTCKSRPEK TKYFKDFLDS WRKFHSALHQ 

        70         80         90        100        110        120 
KEKDVTDSFY PAMRLILPQL ERERMAYGIK ETMLAKLYIE LLNLPKDGKD AVKLLNYRTP 

       130        140        150        160        170        180 
TGSRGDAGDF AMIAYFVLKP RSPKRGRLTV EQVNELLDAI ANNNAAKNKG LVKKSLLQLI 

       190        200        210        220        230        240 
TQSTALEQKW LIRMIIKDLK LGVSQQTIFS IFHPDAAELH NVTTDLEKVC RQLHDPSVSL 

       250        260        270        280        290        300 
SDVSIMLFSA FKPMLAAIAD VQQIEKQMNN QVFYIETKLD GERMQMHKDG DVYKYFSRNG 

       310        320        330        340        350        360 
FDYTQQFGAS PVDGSLTPFI HNVFKSDIQN CILDGEMMAY NPETQTFMQK GNKFDIKRMV 

       370        380        390        400        410        420 
EDSDLQTCFC VFDVLMINDQ KLAHESLSKR YKILSNVFTP LTGRIHVVHK KSARTRKEVI 

       430        440        450        460        470        480 
DALNEAIDNR EEGIMVKDPM STYKPDKRGE GWLKIKPEYV NGLMDELDLL IVGGYWGKGS 

       490        500        510        520        530        540 
RGGMMSHFLC AVAETPAPNE KPTVFHSICR VGSGYTMKEL YDLGLKLAKH WKPYNRKDPP 

       550        560        570        580        590        600 
CNILCGTEKP EMYIEPCNSV IVQIKAAEIV NSDMYKTDCT LRFPRIEKIR EDKEWYECMT 

       610        620        630        640        650        660 
LDMLEHLRSR AEGKLASKHL YIDEYDEPQE KKRRTVPKVK KVIGIAEQFK APDLSNVNKV 

       670        680        690        700        710        720 
SSMFEDVEFC VMTGMGRYSK SELESRIAEC GGSVVQNPGP DTYCVIVGAE NVRVKNIIAS 

       730        740        750        760        770        780 
NKYDVVKAEW LLQCFQSKML VPWQPAFMIH MSPETREHFA REYDCYGDSY TADTDVAQLK 

       790        800        810        820        830        840 
EVFSRVKDNK KMPLDLIAEL EERYSWNSCK LCIFRGNTIY VDYYAIINKP STKIHGTRLS 

       850        860        870        880        890        900 
IRALELRFYG AKVVPLLEEG VSHVVIGEDH SRVKEMKALR RMFGKKFKIV SELWVTESVK 

       910 
EGVPKNETQF LI

« Hide

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References

« Hide 'large scale' references
[1]"DNA ligase IV-deficient cells are more resistant to ionizing radiation in the absence of Ku70: implications for DNA double-strand break repair."
Adachi N., Ishino T., Ishii Y., Takeda S., Koyama H.
Proc. Natl. Acad. Sci. U.S.A. 98:12109-12113(2001) [PubMed: 11593023] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis."
Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M.
Genome Biol. 6:RESEARCH006.1-RESEARCH006.9(2005) [PubMed: 15642098] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: CB.
Tissue: Bursa of Fabricius.

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Cross-references

Sequence databases

AB058600 Genomic DNA. Translation: BAB68506.1.
AJ720061 mRNA. Translation: CAG31720.1.
IPIIPI00651271.
RefSeqNP_001025987.1.
UniGeneGga.1352

3D structure databases

SMRQ90YB1. Positions 659-764.
ModBaseSearch...

Proteomic databases

PRIDEQ90YB1.

Genome annotation databases

EnsemblENSGALG00000016854. Gallus gallus. [Contig view]
GeneID418764.
KEGGgga:418764.

Phylogenomic databases

HOVERGENQ90YB1.

Enzyme and pathway databases

BRENDA6.5.1.1. 4.

Family and domain databases

InterProIPR001357. BRCT.
IPR000977. DNA_ligase.
IPR012309. DNA_ligase_A_C.
IPR012310. DNA_ligase_A_M.
IPR012308. DNA_ligase_A_N.
IPR016059. DNA_ligase_CS.
IPR012340. NA-bd_OB-fold.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PfamPF00533. BRCT. 2 hits.
PF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SMARTSM00292. BRCT. 2 hits.
[Graphical view]
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS50172. BRCT. 2 hits.
PS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameDNLI4_CHICK
AccessionPrimary (citable) accession number: Q90YB1
Secondary accession number(s): Q5ZKM3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: February 7, 2006
Last modified: June 16, 2009
This is version 40 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents