Q90XF2 (KPCI_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein kinase C iota type
EC=2.7.11.13

Alternative name(s):
Atypical protein kinase C-lambda/iota
Short name=aPKC-lambda/iota
Heart and soul protein
nPKC-iota

Gene names

Name:	prkci
Synonyms:	hal

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	588 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Calcium- and diacylglycerol-independent serine/ threonine-protein kinase that plays a general protective role against apoptotic stimuli, is involved in NF-kappa-B activation, cell survival, differentiation and polarity, and contributes to the regulation of microtubule dynamics in the early secretory pathway By similarity. Is required for the formation and maintenance of the zonula adherens during early epithelial development and plays a critical role in organ morphogenesis and in regulating the orientation of cell division. Ref.1
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Exhibits an elevated basal enzymatic activity and is not regulated by diacylglycerol, phosphatidylserine, phorbol esters or calcium ions. Two specific sites, Thr-404 (activation loop of the kinase domain) and Thr-556 (turn motif), need to be phosphorylated for its full activation By similarity.
Domain	The C1 domain does not bind the diacylglycerol (DAG).
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 OPR domain. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 protein kinase domain.
Caution	It is uncertain whether Met-1 or Met-9 is the initiator.
Sequence caution	The sequence AAK91291.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Domain	Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Developmental protein Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	adherens junction maintenance Inferred from mutant phenotype Ref.1. Source: ZFIN cell-cell junction maintenance Inferred from mutant phenotype. Source: ZFIN digestive tract mesoderm development Inferred from mutant phenotype. Source: ZFIN digestive tract morphogenesis Inferred from mutant phenotype Ref.1. Source: ZFIN dorsal motor nucleus of vagus nerve development Inferred from mutant phenotype. Source: ZFIN embryonic heart tube development Inferred from mutant phenotype. Source: ZFIN establishment of mitotic spindle orientation Inferred from mutant phenotype Ref.1. Source: ZFIN establishment or maintenance of polarity of embryonic epithelium Inferred from mutant phenotype. Source: ZFIN intracellular signal transduction Inferred from electronic annotation. Source: InterPro maintenance of epithelial cell apical/basal polarity Inferred from mutant phenotype. Source: ZFIN mesodermal cell migration Inferred from mutant phenotype. Source: ZFIN morphogenesis of a polarized epithelium Inferred from mutant phenotype Ref.1. Source: ZFIN neural tube formation Inferred from genetic interaction. Source: ZFIN neuroblast proliferation Inferred from mutant phenotype. Source: ZFIN nuclear migration Inferred from mutant phenotype. Source: ZFIN retina morphogenesis in camera-type eye Inferred from mutant phenotype. Source: ZFIN
Cellular component	cytosol Inferred from sequence or structural similarity. Source: UniProtKB intrinsic to plasma membrane Inferred from direct assay. Source: ZFIN nucleus Inferred from sequence or structural similarity. Source: UniProtKB zonula adherens Inferred from direct assay Ref.1. Source: ZFIN
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipid binding Inferred from sequence or structural similarity. Source: UniProtKB protein kinase C activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 588	588	Protein kinase C iota type		PRO_0000055713
Regions
	Domain	18 – 101	84	OPR
	Domain	246 – 514	269	Protein kinase
	Domain	515 – 586	72	AGC-kinase C-terminal
	Zinc finger	133 – 183	51	Phorbol-ester/DAG-type
	Nucleotide binding	252 – 260	9	ATP By similarity
Sites
	Active site	370	1	Proton acceptor By similarity
	Binding site	275	1	ATP By similarity
Amino acid modifications
	Modified residue	404	1	Phosphothreonine Probable
	Modified residue	556	1	Phosphothreonine Probable

Sequences

	Sequence		Length	Mass (Da)
	Q90XF2 [UniParc]. Last modified July 5, 2004. Version 2. Checksum: C4E93B83AE67C79B	FASTA	588	67,322
	10 20 30 40 50 60 MPTLRDSTMS HPGENPHQVR VKAYYRGDIM ITHFEPSISY EGLCNEVRDM CSMDNDQLFT 70 80 90 100 110 120 MKWIDEEGDP CTVSSQLELE EALRLYELNK DSELIIHVFP CVPEKPGMPC PGEDKSIYRR 130 140 150 160 170 180 GARRWRKLYY ATGHAFQAKR FNRRAHCAIC TDRIWGLGRQ GYKCINCKLL VHKKCHKLVT 190 200 210 220 230 240 VECGRQVIQD PMIGRIDPGS THPEHPDQVL GKKNSTESIN HEGEEHEAVG SRESGKAVSS 250 260 270 280 290 300 LGLIDFDLLR VIGRGSYAKV LLVRLKKTER IYAMKVVKKE LVNDDEDIDW VQTEKHVFEQ 310 320 330 340 350 360 ASNHPFLVGL HSCFQTESRL FFVIEYVNGG DLMFHMQRQR KLPEEHARFY SAEISLALNY 370 380 390 400 410 420 LHERGIIYRD LKLDNVLLDS EGHIKLTDYG MCKEGLRPGD TTSTFCGTPN YIAPEILRGE 430 440 450 460 470 480 DYGFSVDWWA LGVLMFEMMA GRSPFDIVGS SDNPDQNTED YLFQVILEKQ IRIPRSLSVK 490 500 510 520 530 540 AASVLKGFLN KESKERLGCH PQTGFADIMA HPFFRNVDWD LMEQKQVVPP FKPNISGEFG 550 560 570 580 LDNFDAQFTN EPIQLTPDDD DAVKKIDQSE FEGFEYINPL LMSAEECV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Positional cloning of heart and soul reveals multiple roles for PKC lambda in zebrafish organogenesis." Horne-Badovinac S., Lin D., Waldron S., Schwarz M., Mbamalu G., Pawson T., Jan Y.-N., Stainier D.Y., Abdelilah-Seyfried S. Curr. Biol. 11:1492-1502(2001) [PubMed: 11591316] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: AB.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF390109 mRNA. Translation: AAK91291.1. Different initiation. BC047164 mRNA. Translation: AAH47164.1.
IPI	IPI00497308.
RefSeq	NP_571930.2. NM_131855.2.
UniGene	Dr.2532.
3D structure databases
ProteinModelPortal	Q90XF2.
SMR	Q90XF2. Positions 18-101, 241-580.
ModBase	Search...
Protein-protein interaction databases
STRING	Q90XF2.
Proteomic databases
PRIDE	Q90XF2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000015723; ENSDARP00000005309; ENSDARG00000021225.
GeneID	117507.
KEGG	dre:117507.
Organism-specific databases
CTD	5584.
ZFIN	ZDB-GENE-011105-1. prkci.
Phylogenomic databases
GeneTree	ENSGT00590000082854.
HOGENOM	HBG755340.
HOVERGEN	HBG108317.
InParanoid	Q90XF2.
OMA	GRQGFKC.
OrthoDB	EOG4T782V.
PhylomeDB	Q90XF2.
Gene expression databases
ArrayExpress	Q90XF2.
Bgee	Q90XF2.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR020454. DAG/PE-bd. IPR011009. Kinase-like_dom. IPR000270. OPR_PB1. IPR012233. PKC_zeta. IPR017892. Pkinase_C. IPR002219. Prot_Kinase_C-like_PE/DAG-bd. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
KO	K06069.
Pfam	PF00130. C1_1. 1 hit. PF00564. PB1. 1 hit. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
PIRSF	PIRSF000554. PKC_zeta. 1 hit.
PRINTS	PR00008. DAGPEDOMAIN.
SMART	SM00109. C1. 1 hit. SM00666. PB1. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS00479. ZF_DAG_PE_1. 1 hit. PS50081. ZF_DAG_PE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPCI_DANRE

Accession

Primary (citable) accession number: Q90XF2
Secondary accession number(s): Q7ZU17

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2004
Last sequence update:	July 5, 2004
Last modified:	December 14, 2011
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program