Reviewed, UniProtKB/Swiss-Prot Q90X99 (LYG_EPICO)

Names and origin

Protein names	Recommended name:     Lysozyme g     EC=3.2.1.17 Alternative name(s):     1,4-beta-N-acetylmuramidase
Organism	Epinephelus coioides (Orange-spotted grouper)
Taxonomic identifier	94232 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Neoteleostei › Acanthomorpha › Acanthopterygii › Percomorpha › Perciformes › Percoidei › Serranidae › Epinephelinae › Epinephelus

Protein attributes

Sequence length	194 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has lytic activity against M.lysodeikticus, V.alginolyticus from Epinephelus fario, V.vulnificus from culture water, A.hydrophila from soft-shell turtle, A.hydrophila from goldfish and V.parahaemolyticus, P.fluorescens and V.fluvialis from culture water.
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Tissue specificity	Expressed in intestine, liver, spleen, anterior kidney, posterior kidney, heart, gill, muscle and leukocytes. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 23 family.

Ontologies

Keywords
Molecular function	Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	lysozyme activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 194	194	Lysozyme g		PRO_0000193518
Sites
	Active site	71	1	By similarity
	Active site	84	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q90X99-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 8A12197359127023	FASTA	194	21,179
	10 20 30 40 50 60 MGYGNIMNVE TTGASWQTAQ QDKLGYSGVR ASHTMANTDS GRMERYRSKI NSVGAKYGID 70 80 90 100 110 120 PALIAAIISE ESRAGNVLHD GWGDYDSNRG AYNAWGLMQV DVNPNGGGHT ARGAWDSEEH 130 140 150 160 170 180 LSQGAEILVY FIGRIRNKFP GWNTEQQLKG GIAAYNMGDG NVHSYDNVDG RTTGGDYSND 190 VVARAQWYKT QKGF

References

[1]

"Molecular cloning, expression of orange-spotted grouper goose-type lysozyme cDNA, and lytic activity of its recombinant protein." Yin Z.X., He J.G., Deng W.X., Chan S.M. Dis. Aquat. Organ. 55:117-123(2003) [PubMed: 12911059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF416458 mRNA. Translation: AAL08021.2.
3D structure databases
SMR	Q90X99. Positions 2-194.
ModBase	Search...
Protein family/group databases
CAZy	GH23. Glycoside Hydrolase Family 23.
Phylogenomic databases
HOVERGEN	Q90X99.
Enzyme and pathway databases
BRENDA	3.2.1.17. 290522.
Family and domain databases
InterPro	IPR002152. Glyco_hydro_23. IPR008258. Lytic_TGlycosylase-like_cat. [Graphical view]
Pfam	PF01464. SLT. 1 hit. [Graphical view]
PIRSF	PIRSF001065. Lysozyme_g. 1 hit.
PRINTS	PR00749. LYSOZYMEG.
ProtoNet	Search...

Entry information

Entry name

LYG_EPICO

Accession

Primary (citable) accession number: Q90X99

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	December 1, 2001
Last modified:	January 19, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)