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Protein

G-protein coupled receptor 161

Gene

gpr161

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Key negative regulator of Shh signaling during neural tube development. Recruited to primary cilia and acts as a regulator of the PKA-dependent basal repression machinery in Shh signaling by increasing cAMP levels, leading to promote the PKA-dependent processing of gli3 into gli3r and repress the Shh signaling. In presence of shh, it is removed from primary cilia, preventing its activity and allowing activation of the Shh signaling (By similarity). Required in left/right patterning by modulating Ca2+ levels in the cells surrounding the Kupffer vesicle.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-DRE-5610787. Hedgehog 'off' state.
R-DRE-5632684. Hedgehog 'on' state.

Names & Taxonomyi

Protein namesi
Recommended name:
G-protein coupled receptor 161
Gene namesi
Name:gpr161
ORF Names:si:rp71-20i5.4
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 9

Organism-specific databases

ZFINiZDB-GENE-030616-58. gpr161.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2727ExtracellularSequence analysisAdd
BLAST
Transmembranei28 – 4821Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini49 – 6012CytoplasmicSequence analysisAdd
BLAST
Transmembranei61 – 8121Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini82 – 9817ExtracellularSequence analysisAdd
BLAST
Transmembranei99 – 11921Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini120 – 13920CytoplasmicSequence analysisAdd
BLAST
Transmembranei140 – 16021Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini161 – 18626ExtracellularSequence analysisAdd
BLAST
Transmembranei187 – 20721Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini208 – 26356CytoplasmicSequence analysisAdd
BLAST
Transmembranei264 – 28421Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini285 – 30016ExtracellularSequence analysisAdd
BLAST
Transmembranei301 – 32121Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini322 – 526205CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cilium, Membrane

Pathology & Biotechi

Disruption phenotypei

Embryos exhibit perturb cardiac looping as the result of a defect in left/right patterning.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 526526G-protein coupled receptor 161PRO_0000379074Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi2 – 21N-linked (GlcNAc...)Sequence analysis
Glycosylationi6 – 61N-linked (GlcNAc...)Sequence analysis
Glycosylationi12 – 121N-linked (GlcNAc...)Sequence analysis
Disulfide bondi96 ↔ 174PROSITE-ProRule annotation
Glycosylationi97 – 971N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ90X46.

Expressioni

Developmental stagei

Expressed throughout embryonic development. At 9- to 16-somites, transcripts are broadly expressed with specific staining observed in the developing nervous system, somites, and precardiac mesoderm. At later stages 1- to 3-days post-fertilization, transcripts become more localized within the dorsal diencephalon, the otic vesicles, and the fin buds.

Gene expression databases

BgeeiENSDARG00000055659.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000118097.

Structurei

3D structure databases

ProteinModelPortaliQ90X46.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi30 – 356Poly-Ile
Compositional biasi512 – 5165Poly-Asp

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00790000123027.
HOGENOMiHOG000112777.
InParanoidiQ90X46.
KOiK08439.
OMAiIWCALLP.
OrthoDBiEOG091G0G56.
PhylomeDBiQ90X46.
TreeFamiTF331895.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q90X46-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGSKNGTAV ANSTNGLDDN GLMVLESVSI IIIAILACLG NLVIVVTLYK
60 70 80 90 100
KPYLLTPSNK FVFSLTSSNL LLSVLMLPFV VASSVRRDWM FGVVWCNFTA
110 120 130 140 150
LLHLLVSSSS MLTLGAIAID RYYAVLYPMI YPMKITGNRA VLAIVYIWLH
160 170 180 190 200
SLVGCLPPLF GWSSFEFDRF KWTCTVSWHK EISYTAFWVT WCCLLPLVAM
210 220 230 240 250
LVCYGVIFRV ARIKARKVYC GSVVVSQEES SSQNNGRKNS NTSTSSSGSR
260 270 280 290 300
KSLIYSGSQC KAFITILVVL GTFLTTWGPY VVVISTEALL GKNSVSPQVE
310 320 330 340 350
TLVSWLSFTS AVCHPLIYGL WNKTVRKELL GMCFDDRYYR ESFVIRHRTS
360 370 380 390 400
RLFSISNRIT DLGMSPHLTA MLVGGGQLLG RGSSTGDTGF SYTQDSATDV
410 420 430 440 450
MLLESYTSEA SHSAHCTANK RRSSVTFEDQ VDHIPQGDPS VVQVTADIHK
460 470 480 490 500
SLDSFASSLA KAIENDAKLQ LLGEWTQIPT SLFTVRNTQR VPRYLDGQRL
510 520
RMESIDEGIV KDDDDDEEEM EREEKM
Length:526
Mass (Da):58,485
Last modified:March 1, 2003 - v2
Checksum:iCACE795D638B5B3E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti84 – 841S → T in AAI35012 (Ref. 3) Curated
Sequence conflicti519 – 5191E → EE in AAI35012 (Ref. 3) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU090912 mRNA. Translation: ABW77593.1.
AL590146 Genomic DNA. Translation: CAC94897.2.
BC135011 mRNA. Translation: AAI35012.1.
RefSeqiNP_001007200.2. NM_001007199.2.
UniGeneiDr.88054.

Genome annotation databases

EnsembliENSDART00000078051; ENSDARP00000072514; ENSDARG00000055659.
ENSDART00000143991; ENSDARP00000118097; ENSDARG00000055659.
GeneIDi368421.
KEGGidre:368421.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU090912 mRNA. Translation: ABW77593.1.
AL590146 Genomic DNA. Translation: CAC94897.2.
BC135011 mRNA. Translation: AAI35012.1.
RefSeqiNP_001007200.2. NM_001007199.2.
UniGeneiDr.88054.

3D structure databases

ProteinModelPortaliQ90X46.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000118097.

Protein family/group databases

GPCRDBiSearch...

Proteomic databases

PaxDbiQ90X46.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000078051; ENSDARP00000072514; ENSDARG00000055659.
ENSDART00000143991; ENSDARP00000118097; ENSDARG00000055659.
GeneIDi368421.
KEGGidre:368421.

Organism-specific databases

CTDi23432.
ZFINiZDB-GENE-030616-58. gpr161.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00790000123027.
HOGENOMiHOG000112777.
InParanoidiQ90X46.
KOiK08439.
OMAiIWCALLP.
OrthoDBiEOG091G0G56.
PhylomeDBiQ90X46.
TreeFamiTF331895.

Enzyme and pathway databases

ReactomeiR-DRE-5610787. Hedgehog 'off' state.
R-DRE-5632684. Hedgehog 'on' state.

Miscellaneous databases

PROiQ90X46.

Gene expression databases

BgeeiENSDARG00000055659.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGP161_DANRE
AccessioniPrimary (citable) accession number: Q90X46
Secondary accession number(s): A4IGB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.