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Protein

ATP-dependent RNA helicase DDX1

Gene

DDX1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Acts as a positive regulator of transcription. May be involved in 3'-end cleavage and polyadenylation of pre-mRNAs. Binds DNA and RNA. Component of the tRNA-splicing ligase complex required to facilitate the enzymatic turnover of catalytic subunit RTCB (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi46 – 53ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Exonuclease, Helicase, Hydrolase, Nuclease

Keywords - Biological processi

mRNA processing, Transcription, Transcription regulation, tRNA processing

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DDX1 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 1
Gene namesi
Name:DDX1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003123591 – 740ATP-dependent RNA helicase DDX1Add BLAST740

Proteomic databases

PaxDbiQ90WU3.
PRIDEiQ90WU3.

Expressioni

Tissue specificityi

Detected in embryonic retina, brain, heart and liver (at protein level). Detected in embryonic retina, brain, heart, kidney and liver.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi675543. 1 interactor.
STRINGi9031.ENSGALP00000036098.

Structurei

3D structure databases

ProteinModelPortaliQ90WU3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 428Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST427
Domaini70 – 247B30.2/SPRYPROSITE-ProRule annotationAdd BLAST178
Domaini493 – 681Helicase C-terminalPROSITE-ProRule annotationAdd BLAST189

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi370 – 373DEAD box4

Domaini

The helicase domain is involved in the stimulation of RELA transcriptional activity.By similarity

Sequence similaritiesi

Contains 1 B30.2/SPRY domain.PROSITE-ProRule annotation
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0349. Eukaryota.
COG0513. LUCA.
HOGENOMiHOG000251633.
HOVERGENiHBG005462.
InParanoidiQ90WU3.
KOiK13177.
PhylomeDBiQ90WU3.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
InterProiIPR001870. B30.2/SPRY.
IPR013320. ConA-like_dom.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
IPR003877. SPRY_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00622. SPRY. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF52540. SSF52540. 3 hits.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 2 hits.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q90WU3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAFSEMGVM PEIAQAVEEM DWLLPTDIQA ESIPLILGGG DVLMAAETGS
60 70 80 90 100
GKTGAFSIPV IQIVYETLKD QMEGKKGKAT IKTGGAVLNK WQMNPYDRGS
110 120 130 140 150
AFAIGSDGLC CQSREVKEWH GCRATRGVTK GKYYYEVSCH DQGLCRVGWS
160 170 180 190 200
TMQASLDLGT DKFGFGFGGT GKKSHNKQFD SYGEEFTMHD TIGCYLDIDK
210 220 230 240 250
GQIKFSKNGK DLGLAFEFPP HIRNQALFAA CVLKNAELKF NFGEEDFKFP
260 270 280 290 300
PKDGYIGLCK APDGNVVKSQ HTGNAQVVQT QNLPNAPKAL IVEPSRELAE
310 320 330 340 350
QTLNNVKQFK KYIDNPKLRE LLIIGGVAAR DQLSVLEQGV DIVVGTPGRL
360 370 380 390 400
DDLVSTGKLN LSQVRFLVLD EADGLLLQGY SDFINRIHSQ IPQITSDGKR
410 420 430 440 450
LQVIVCSATL HSFDVKKLSE KIMHFPTWVD LKGEDSVPET VHHVVVIVNP
460 470 480 490 500
KTDKLWERLG KNHIRTDEVH AKDNTLPGAN TPEMWSEAIK ILKGEYTVRA
510 520 530 540 550
IKEHKMDQAI IFCRTKIDCD NMEQYFIQQG GGPDRKGHQF SCVCLHGDRK
560 570 580 590 600
PQERKQNLER FKRGDVRFLI CTDVAARGID IHGVPYVINV TLPDEKQNYV
610 620 630 640 650
HRIGRVGRAE RMGLAISLVA KEKEKVWYHV CSSRGKGCYN TRLKEEGGCT
660 670 680 690 700
IWYNEMQLLG EIEEHLNCTI SQVEPDIKVP VDDFDGKVTY GQKRALGGGL
710 720 730 740
YKGHVDILAP TVQELAALEK EAQTSFLHLG YLPNQLFRTF
Length:740
Mass (Da):82,484
Last modified:December 1, 2001 - v1
Checksum:iDAC1AC7B3D305365
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY057383 mRNA. Translation: AAL15417.1.
RefSeqiNP_989894.1. NM_204563.1.
UniGeneiGga.14606.

Genome annotation databases

GeneIDi395249.
KEGGigga:395249.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY057383 mRNA. Translation: AAL15417.1.
RefSeqiNP_989894.1. NM_204563.1.
UniGeneiGga.14606.

3D structure databases

ProteinModelPortaliQ90WU3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi675543. 1 interactor.
STRINGi9031.ENSGALP00000036098.

Proteomic databases

PaxDbiQ90WU3.
PRIDEiQ90WU3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi395249.
KEGGigga:395249.

Organism-specific databases

CTDi1653.

Phylogenomic databases

eggNOGiKOG0349. Eukaryota.
COG0513. LUCA.
HOGENOMiHOG000251633.
HOVERGENiHBG005462.
InParanoidiQ90WU3.
KOiK13177.
PhylomeDBiQ90WU3.

Miscellaneous databases

PROiQ90WU3.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
InterProiIPR001870. B30.2/SPRY.
IPR013320. ConA-like_dom.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
IPR003877. SPRY_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00622. SPRY. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF52540. SSF52540. 3 hits.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 2 hits.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDDX1_CHICK
AccessioniPrimary (citable) accession number: Q90WU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: December 1, 2001
Last modified: July 6, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.