Reviewed, UniProtKB/Swiss-Prot Q90VZ3 (LYG_PAROL)

Names and origin

Protein names	Recommended name:     Lysozyme g     EC=3.2.1.17 Alternative name(s):     1,4-beta-N-acetylmuramidase
Organism	Paralichthys olivaceus (Japanese flounder)
Taxonomic identifier	8255 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Neoteleostei › Acanthomorpha › Acanthopterygii › Percomorpha › Pleuronectiformes › Pleuronectoidei › Paralichthyidae › Paralichthys

Protein attributes

Sequence length	195 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Possesses lytic activity against M.lysodeikticus and several fish pathogenic bacteria.
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Tissue specificity	Ubiquitous. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 23 family.
Biophysicochemical properties	pH dependence: Optimum pH is 6.0. Temperature dependence: Optimum temperature is 25 degrees Celsius.

Ontologies

Keywords
Molecular function	Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	lysozyme activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 195	195	Lysozyme g		PRO_0000193520
Sites
	Active site	71	1	By similarity
	Active site	84	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q90VZ3-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 511DAF31876F662F	FASTA	195	21,385
	10 20 30 40 50 60 MSYGQIRLVE TSGASGATSQ QDNLGYSGVK ASHKMAEIDS GRMSKYKSKI NKVGQSYGIE 70 80 90 100 110 120 PALIAAIISR ESRAGNQLKD GWGDWNPQRQ AYNAWGLMQV DVNPNGGGHT AVGGWDSEDH 130 140 150 160 170 180 LRQATGILVT FIERIRTKFP GWSKEKQLKG GIAAYNMGDK NVHSYEGVDE NTTGRDYSND 190 VTARAQWYRD NGYSG

References

[1]

"Molecular cloning, expression and evolution of the Japanese flounder goose-type lysozyme gene, and the lytic activity of its recombinant protein." Hikima J., Minagawa S., Hirono I., Aoki T. Biochim. Biophys. Acta 1520:35-44(2001) [PubMed: 11470157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], CHARACTERIZATION, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB050591 Genomic DNA. Translation: BAB62407.1. AB050590 mRNA. Translation: BAB62406.1.
3D structure databases
HSSP	HSSP built from PDB template 153L based on UniProtKB P00718.
ModBase	Search...
Protein family/group databases
CAZy	GH23. Glycoside Hydrolase Family 23.
Phylogenomic databases
HOVERGEN	Q90VZ3.
Enzyme and pathway databases
BRENDA	3.2.1.17. 74107.
Family and domain databases
InterPro	IPR002152. Glyco_hydro_23. IPR008258. Lytic_TGlycosylase-like_cat. [Graphical view]
Pfam	PF01464. SLT. 1 hit. [Graphical view]
PIRSF	PIRSF001065. Lysozyme_g. 1 hit.
PRINTS	PR00749. LYSOZYMEG.
ProtoNet	Search...

Entry information

Entry name

LYG_PAROL

Accession

Primary (citable) accession number: Q90VZ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	December 1, 2001
Last modified:	June 16, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)