Reviewed, UniProtKB/Swiss-Prot Q90891 (MP2K2_CHICK)

Names and origin

Protein names

Recommended name:
    Dual specificity mitogen-activated protein kinase kinase 2
      Short name=MAP kinase kinase 2
      Short name=MAPKK 2
    EC=2.7.12.2
Alternative name(s):
    ERK activator kinase 2
    MAPK/ERK kinase 2
      Short name=MEK2

Gene names

Name:	MAP2K2
Synonyms:	MEK2, MKK2, PRKMK2

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Post-translational modification	Activated by phosphorylation on Ser/Thr catalyzed by MAP kinase kinase kinases (RAF) By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	Dual specificity mitogen-activated protein kinase kinase 2		PRO_0000086375
Regions
	Domain	70 – 367	298	Protein kinase
	Nucleotide binding	76 – 84	9	ATP By similarity
	Compositional bias	264 – 313	50	Pro-rich
Sites
	Active site	192	1	Proton acceptor By similarity
	Binding site	99	1	ATP By similarity
Amino acid modifications
	Modified residue	220	1	Phosphoserine; by RAF By similarity
	Modified residue	224	1	Phosphoserine; by RAF By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q90891-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 84BDD4C9797D62D1	FASTA	398	44,078
	10 20 30 40 50 60 MPAKRKPVLP ALTITPSPAE GPGPGGSAEA NLVDLQKKLE ELELDEQQKK RLEAFLTQKA 70 80 90 100 110 120 KVGELKDDDF ERISELGAGN GGVVTKVQHK PSGLIMARKL IHLEIKPAIR NQIIRELQVL 130 140 150 160 170 180 HECNSPYIVG FYGAFYSDGE ISICMEHMDG GSLDQVLKEA KRIPEEILGK VSIAVLRGLA 190 200 210 220 230 240 YLREKHQIMH RDVKPSNILV NSRGEIKLCD FGVSGQLIDS MANSFVGTRS YMSPERLQGT 250 260 270 280 290 300 HYSVQSDIWS MGLSLVELSI GRYPIPPPDS KELEAIFGRP VVDGAEGESH SVSPWARPPG 310 320 330 340 350 360 RPISGHGMDS RPAMAIFELL DYIVNEPPPK LPNGVFTQDF QEFVNKCLIK NPAERADLKM 370 380 390 LMNHTFIKRS EVEEVDFAGW LCKTLRLNQP STPTRAAV

References

[1]

"Cloning and characterization of cDNAs encoding chicken mitogen-activated protein kinase kinase type 2, MEK2: downregulation of MEK2 in response to inhibition of mitochondrial DNA expression." Wang H., Meury L., Morais R. Biochemistry 36:15371-15380(1997) [PubMed: 9398267] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: White leghorn. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L28703 mRNA. Translation: AAA75576.1.
IPI	IPI00596185.
RefSeq	NP_990719.1.
UniGene	Gga.2391
3D structure databases
HSSP	HSSP built from PDB template 1BI8 based on UniProtKB Q00534.
SMR	Q90891. Positions 58-390.
ModBase	Search...
Protein-protein interaction databases
STRING	Q90891.
Proteomic databases
PRIDE	Q90891.
Genome annotation databases
Ensembl	ENSGALT00000001934; ENSGALP00000001932; ENSGALG00000001267; Gallus gallus. [Genome view]
GeneID	396349.
KEGG	gga:396349.
Organism-specific databases
CTD	396349.
Phylogenomic databases
HOGENOM	Q90891.
HOVERGEN	Q90891.
Enzyme and pathway databases
BRENDA	2.7.12.2. 4.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MP2K2_CHICK

Accession

Primary (citable) accession number: Q90891

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	September 1, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)