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Protein

Actin filament-associated protein 1

Gene

AFAP1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Function as an adapter molecule that links other proteins to the actin cytoskeleton. May function in facilitating interactions between SRC and actin filaments. May modulate changes in actin filament integrity and induce lamellipodia formation. Can cross-link actin filaments into both network and bundle structures.

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Actin filament-associated protein 1
Alternative name(s):
110 kDa actin filament-associated protein
Short name:
AFAP-110
Neural actin filament protein
pp110
Gene namesi
Name:AFAP1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

  • Cytoplasmcytoskeleton 3 Publications

  • Note: Localizes with stress fibers in quiescent cells, concentrated in cell motility structures such as lamellipodia, filopodia and membrane ruffles upon their induction.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi71 – 711P → A: Abolishes interaction with SRC SH3 domain and decreases tyrosine phosphorylation. 1 Publication
Mutagenesisi93 – 931Y → A: Reduces phosphorylation and abolishes interaction with activated SRC; when associated with A-94; A-451 and A-453. 1 Publication
Mutagenesisi94 – 941Y → A: Reduces phosphorylation and abolishes interaction with activated SRC; when associated with A-93; A-451 and A-453. 1 Publication
Mutagenesisi451 – 4511Y → A: Reduces phosphorylation and abolishes interaction with activated SRC; when associated with A-93; A-94 and A-451. 1 Publication
Mutagenesisi453 – 4531Y → A: Reduces phosphorylation and abolishes interaction with activated SRC; when associated with A-93; A-94 and A-451. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 729729Actin filament-associated protein 1PRO_0000317661Add
BLAST

Post-translational modificationi

Phosphorylated on tyrosine residues by SRC.3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ90738.

Expressioni

Tissue specificityi

Isoform 2 is specifically expressed in brain.1 Publication

Interactioni

Subunit structurei

Monomer and homomultimer. Interacts via its C-terminus with F-actin; probably involving AFAP1 multimers. Interacts with activated SRC SH3-SH2 domains. May interact with FYN SH3-SH2 domains. Interacts via its PH 1 domain with PRKCA, PRKCB, PRKCI.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SRCP005233EBI-8562073,EBI-848039

Protein-protein interaction databases

IntActiQ90738. 1 interaction.
MINTiMINT-8013618.
STRINGi9031.ENSGALP00000025034.

Structurei

3D structure databases

ProteinModelPortaliQ90738.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini153 – 24997PH 1PROSITE-ProRule annotationAdd
BLAST
Domaini347 – 44195PH 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni594 – 63744Interaction with F-actinAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili557 – 64892Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi71 – 744SH3-binding
Motifi94 – 974SH2-binding 1
Motifi451 – 4566SH2-binding 2

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi60 – 10647Pro-richAdd
BLAST

Sequence similaritiesi

Contains 2 PH domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, SH3-binding

Phylogenomic databases

eggNOGiENOG410IEU6. Eukaryota.
ENOG4111CMN. LUCA.
HOGENOMiHOG000033832.
HOVERGENiHBG106875.
InParanoidiQ90738.
KOiK18616.
PhylomeDBiQ90738.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR030113. AFAP.
IPR029907. AFAP-110.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PANTHERiPTHR14338. PTHR14338. 1 hit.
PTHR14338:SF8. PTHR14338:SF8. 1 hit.
PfamiPF00169. PH. 2 hits.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 4 hits.
PROSITEiPS50003. PH_DOMAIN. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q90738-1) [UniParc]FASTAAdd to basket
Also known as: AFAP-110

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEELIVELRL FLELLDHEYL TSTVREKKAV ITNILLRIQS SKGFEIKEHV
60 70 80 90 100
QKPEVANSLP APPQMPLPEI PQQWLPPDNG PPPLPTSSLP EGYYEEAVPV
110 120 130 140 150
SPGKAPEYIT SNYDSDAMSS SYESYDEEEE DGKGKKMRHQ WPSEEASMDL
160 170 180 190 200
VKDAKICAFL LRKKRFGQWT KLLCVIKENK LLCYKSSKDQ QPQMELLLND
210 220 230 240 250
CSITYIPKDS KKKKHELKIS HQGADALVLA VQSKEQAEQW LKVIKDVCSN
260 270 280 290 300
CTGTVDSDGP LSSSPVHKTE LEKKLSSERP SSDGEGAVEN GITTVCNGKE
310 320 330 340 350
QVKRKKSSKT EAKGTVTKVT GKKITKIIGL GKKKPSTDEQ TSSAEEDVPT
360 370 380 390 400
CGYLNVLSNN RWRERWCRVK DNKLIFHKDR TDLKTHIVSI PLRGCEVIPG
410 420 430 440 450
LDSKHPLTFR LLRNGQEVAV LEASSSEDMG RWIGMLLAET GSSTDPGALH
460 470 480 490 500
YDYIDVEMTA SVIQAAKQTF CFMNRRVIST NPYRGSTANG YACPSGMALH
510 520 530 540 550
YDDVPCINGS FKGKKPPATA NGITGKVRTL NSQPKKPESV SCVKRTASNA
560 570 580 590 600
EQYKYGKNRV EAVAKRLQSK EEELLKRKEA LRNRLAQLRK ERKDLRAAIE
610 620 630 640 650
VNAGRKTQVI LEDKLKKLEE ECKTKEAERV NLELELTEVK ESLKKALAGG
660 670 680 690 700
ITLGLAIEPK SGTSSPQSPI FKHRTLENSP ISSCDTSDTE CSIPVNSAAA
710 720
LKRPPSSNNS PCRGHVLRKA KEWEMKNGT
Length:729
Mass (Da):81,160
Last modified:June 1, 2001 - v2
Checksum:i4735A746E665890B
GO
Isoform 2 (identifier: Q90738-2) [UniParc]FASTAAdd to basket
Also known as: AFAP-120

The sequence of this isoform differs from the canonical sequence as follows:
     510-510: S → SWEPEDGFPS...SAVGRGSVSQ

Show »
Length:815
Mass (Da):90,646
Checksum:iC6EF6E639DF5F256
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei510 – 5101S → SWEPEDGFPSSRSRNMGEEM LYDNAGLYDNLPSPKIFARY PPADRKTNRLSTDKLSSNHY KHPVSSNLSSAQSVTNTSAV GRGSVSQ in isoform 2. 1 PublicationVSP_031121

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20303 mRNA. Translation: AAA18166.2.
L20302 mRNA. Translation: AAA67326.2.
PIRiA54592.
A55883.
RefSeqiNP_001128120.1. NM_001134648.1. [Q90738-1]
NP_989536.1. NM_204205.1. [Q90738-2]
UniGeneiGga.185.

Genome annotation databases

GeneIDi374034.
KEGGigga:374034.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20303 mRNA. Translation: AAA18166.2.
L20302 mRNA. Translation: AAA67326.2.
PIRiA54592.
A55883.
RefSeqiNP_001128120.1. NM_001134648.1. [Q90738-1]
NP_989536.1. NM_204205.1. [Q90738-2]
UniGeneiGga.185.

3D structure databases

ProteinModelPortaliQ90738.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ90738. 1 interaction.
MINTiMINT-8013618.
STRINGi9031.ENSGALP00000025034.

Proteomic databases

PaxDbiQ90738.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi374034.
KEGGigga:374034.

Organism-specific databases

CTDi60312.

Phylogenomic databases

eggNOGiENOG410IEU6. Eukaryota.
ENOG4111CMN. LUCA.
HOGENOMiHOG000033832.
HOVERGENiHBG106875.
InParanoidiQ90738.
KOiK18616.
PhylomeDBiQ90738.

Miscellaneous databases

PROiQ90738.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR030113. AFAP.
IPR029907. AFAP-110.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PANTHERiPTHR14338. PTHR14338. 1 hit.
PTHR14338:SF8. PTHR14338:SF8. 1 hit.
PfamiPF00169. PH. 2 hits.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 4 hits.
PROSITEiPS50003. PH_DOMAIN. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAFAP1_CHICK
AccessioniPrimary (citable) accession number: Q90738
Secondary accession number(s): Q90917
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: June 1, 2001
Last modified: June 8, 2016
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.