Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q90703

- NOS2_CHICK

UniProt

Q90703 - NOS2_CHICK

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Nitric oxide synthase, inducible

Gene

NOS2

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO may serve as both a paracrine and autocrine signal for modulating osteoclast bone resorption. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 (By similarity).By similarity

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Protein has several cofactor binding sites:
  • hemeBy similarity
  • FADBy similarityNote: Binds 1 FAD.By similarity
  • FMNBy similarityNote: Binds 1 FMN.By similarity
  • 5,6,7,8-tetrahydrobiopterinBy similarityNote: Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.By similarity

Enzyme regulationi

Not stimulated by calcium/calmodulin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi107 – 1071ZincBy similarity
Metal bindingi112 – 1121ZincBy similarity
Metal bindingi197 – 1971Iron (heme axial ligand)By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi620 – 65132FMNPROSITE-ProRule annotationAdd
BLAST
Nucleotide bindingi764 – 77512FADBy similarityAdd
BLAST
Nucleotide bindingi900 – 91011FADBy similarityAdd
BLAST
Nucleotide bindingi975 – 99319NADPBy similarityAdd
BLAST
Nucleotide bindingi1073 – 108816NADPBy similarityAdd
BLAST

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: InterPro
  2. FMN binding Source: InterPro
  3. heme binding Source: InterPro
  4. iron ion binding Source: InterPro
  5. NADP binding Source: InterPro
  6. nitric-oxide synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. nitric oxide biosynthetic process Source: InterPro
  2. peptidyl-cysteine S-nitrosylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase, inducible (EC:1.14.13.39)
Alternative name(s):
Inducible NO synthase
Short name:
Inducible NOS
Short name:
iNOS
Macrophage NOS
NOS type II
Peptidyl-cysteine S-nitrosylase NOS2
Gene namesi
Name:NOS2
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11361136Nitric oxide synthase, induciblePRO_0000170938Add
BLAST

Proteomic databases

PaxDbiQ90703.
PRIDEiQ90703.

Expressioni

Inductioni

By treatment with lipopolysaccharides (LPS) or cytokines, but not in osteoclasts where they are induced by calcium and PMA (phorbol 12-myristate 13-acetate).

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ90703.
SMRiQ90703. Positions 80-499, 700-1127.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini536 – 674139Flavodoxin-likePROSITE-ProRule annotationAdd
BLAST
Domaini727 – 967241FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni506 – 52621Calmodulin-bindingSequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the NOS family.Curated
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG4362.
HOGENOMiHOG000220884.
HOVERGENiHBG000159.
InParanoidiQ90703.
KOiK13241.
PhylomeDBiQ90703.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
3.90.340.10. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000333. NOS. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q90703-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLCPWQFAFK PHAVKNQSSE EKDINNNVEK DVKVHSFVKD DAKLHSLSKK
60 70 80 90 100
QMKMSPIITS AEKHPQNGIK ASNQISRCPR HVKVRNMENG SSLLDTLHLT
110 120 130 140 150
AKEVINCRTR ACQGALMTPK GLVRSTRDGP VPPAELLPQA VDFVKQYYSS
160 170 180 190 200
FKELKIEEHL ARLETVTKEI ETTGTYHLTK DELIFAAKQA WRNAPRCIGR
210 220 230 240 250
IQWSNLQVFD ARDCKTAKEM FEYICRHIQY ATNNGNIRSA ITIFPQRTDG
260 270 280 290 300
KHDFRVWNSQ LIRYAGYQMP DGSVIGDPAS VEFTKLCIEL GWKPKYGRFD
310 320 330 340 350
VVPLILQANG QDPEIFEYPP EIILEVPMEH PKYEWFKELD LKWYALPAVA
360 370 380 390 400
NMLLEVGGLE FTACPFNGWY MGTEIGVRDF CDVQRYNILK EVGRRMGLES
410 420 430 440 450
NKLASLWKDR AVVEINVAVL HSFQKQNVTI MDHHSAAESF MKYMQNEYRV
460 470 480 490 500
RGGCPADWVW IVPPMSGSIT PVFHQEMLNY VLTPFFYYQV DAWKTHIWHD
510 520 530 540 550
ETRRPKKREI KLSILAKAVL LASLLLQKTM AARPKVTVIY ATETGKSETL
560 570 580 590 600
ANSLCSLFSC AFNTKILCMD EYNISDLEKE TLLLVVTSTF GNGDSPNNGK
610 620 630 640 650
TLKNSLLTLK LLRKNIRYAV FGLGSTMYPE FCAFAHAIDQ KLSQLGALQL
660 670 680 690 700
TPVGEGDELN GQEEAFRTWA VTAFKTACDI FDIRGKNSIQ LPEIYTSDDS
710 720 730 740 750
WNPKKHRIVY DSQTMDLTKA LSDIHGKNVI PMKLKFRQNL QSLKSSRVTI
760 770 780 790 800
LVKLSCETNQ EVHYLPGEHI GISPGNQPEL VHGLIARVKD APPADQTIRL
810 820 830 840 850
ETCTEGGYWA SEKKIPACTL SQALTYLLDI TTPPTQQLLK KLSQLVTAEG
860 870 880 890 900
DKQRLEVLCH STEEYNKWKF YNRPNILEVL EEFPSAEVST AFLLTQLPLL
910 920 930 940 950
KPRYYSVSSS CDMTPREIHL TVAVVNYRTR DGQGPLHHGV CSTWLNKIAL
960 970 980 990 1000
NETVPCFVRS ADGFRLPKEP AKPCILIGPG TGIAPFRSFW QQRLYDLEKK
1010 1020 1030 1040 1050
GIKGGDMILL FGCRHPDMDH IYKEEVEEMK RKGVLKEVFT AYSRQPGQPK
1060 1070 1080 1090 1100
VYVQDILQNE LETKVCNILH KEEGHLYVCG DVRMARDVAQ TLKRMLVKKL
1110 1120 1130
NHTEQQAEEY FFQLKSQKRY HEDIFGAVFP HEVKRI
Length:1,136
Mass (Da):129,649
Last modified:November 1, 1997 - v1
Checksum:iD5CB73AC7BA94B9D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti400 – 4001S → T in AAB17499. (PubMed:8707887)Curated
Sequence conflicti873 – 8731R → S in BAA12817. (PubMed:9709401)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U46504 mRNA. Translation: AAC59886.1.
U34045 mRNA. Translation: AAB17499.1.
D85422 mRNA. Translation: BAA12817.1.
RefSeqiNP_990292.1. NM_204961.1.
UniGeneiGga.3327.

Genome annotation databases

GeneIDi395807.
KEGGigga:395807.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U46504 mRNA. Translation: AAC59886.1 .
U34045 mRNA. Translation: AAB17499.1 .
D85422 mRNA. Translation: BAA12817.1 .
RefSeqi NP_990292.1. NM_204961.1.
UniGenei Gga.3327.

3D structure databases

ProteinModelPortali Q90703.
SMRi Q90703. Positions 80-499, 700-1127.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q90703.
PRIDEi Q90703.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 395807.
KEGGi gga:395807.

Organism-specific databases

CTDi 4843.

Phylogenomic databases

eggNOGi COG4362.
HOGENOMi HOG000220884.
HOVERGENi HBG000159.
InParanoidi Q90703.
KOi K13241.
PhylomeDBi Q90703.

Miscellaneous databases

NextBioi 20815875.
PROi Q90703.

Family and domain databases

Gene3Di 1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
3.90.340.10. 1 hit.
InterProi IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000333. NOS. 1 hit.
PRINTSi PR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMi SSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEi PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and expression of an avian macrophage nitric-oxide synthase cDNA and the analysis of the genomic 5'-flanking region."
    Lin A.W., Chang C.C., McCormick C.C.
    J. Biol. Chem. 271:11911-11919(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Proinflammatory agents, IL-8 and IL-10, upregulate inducible nitric oxide synthase expression and nitric oxide production in avian osteoclast-like cells."
    Sunyer T., Rothe L., Jiang X., Osdoby P., Collin-Osdoby P.
    J. Cell. Biochem. 60:469-483(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 171-472.
    Tissue: Osteoclast.
  3. "Molecular cloning and expression of inducible nitric oxide synthase in chick embryonic ventricular myocytes."
    Shimizu T., Kinugawa K., Sugishita Y., Sugishita K., Harada K., Matsui H., Kohmoto O., Serizawa T., Takahashi T.
    Cardiovasc. Res. 38:405-413(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 646-986.
    Tissue: Heart.

Entry informationi

Entry nameiNOS2_CHICK
AccessioniPrimary (citable) accession number: Q90703
Secondary accession number(s): Q90677, Q90934
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 26, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3