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Q90703 (NOS2_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nitric oxide synthase, inducible

EC=1.14.13.39
Alternative name(s):
Inducible NO synthase
Short name=Inducible NOS
Short name=iNOS
Macrophage NOS
NOS type II
Peptidyl-cysteine S-nitrosylase NOS2
Gene names
Name:NOS2
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus

Protein attributes

Sequence length1136 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO may serve as both a paracrine and autocrine signal for modulating osteoclast bone resorption. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 By similarity.

Catalytic activity

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor

Heme group By similarity.

Binds 1 FAD By similarity.

Binds 1 FMN By similarity.

Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.

Enzyme regulation

Not stimulated by calcium/calmodulin.

Subunit structure

Homodimer By similarity.

Induction

By treatment with lipopolysaccharides (LPS) or cytokines, but not in osteoclasts where they are induced by calcium and PMA (phorbol 12-myristate 13-acetate).

Sequence similarities

Belongs to the NOS family.

Contains 1 FAD-binding FR-type domain.

Contains 1 flavodoxin-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11361136Nitric oxide synthase, inducible
PRO_0000170938

Regions

Domain536 – 674139Flavodoxin-like
Domain727 – 967241FAD-binding FR-type
Nucleotide binding620 – 65132FMN By similarity
Nucleotide binding764 – 77512FAD By similarity
Nucleotide binding900 – 91011FAD By similarity
Nucleotide binding975 – 99319NADP By similarity
Nucleotide binding1073 – 108816NADP By similarity
Region506 – 52621Calmodulin-binding Potential

Sites

Metal binding1071Zinc By similarity
Metal binding1121Zinc By similarity
Metal binding1971Iron (heme axial ligand) By similarity

Experimental info

Sequence conflict4001S → T in AAB17499. Ref.2
Sequence conflict8731R → S in BAA12817. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q90703 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: D5CB73AC7BA94B9D

FASTA1,136129,649
        10         20         30         40         50         60 
MLCPWQFAFK PHAVKNQSSE EKDINNNVEK DVKVHSFVKD DAKLHSLSKK QMKMSPIITS 

        70         80         90        100        110        120 
AEKHPQNGIK ASNQISRCPR HVKVRNMENG SSLLDTLHLT AKEVINCRTR ACQGALMTPK 

       130        140        150        160        170        180 
GLVRSTRDGP VPPAELLPQA VDFVKQYYSS FKELKIEEHL ARLETVTKEI ETTGTYHLTK 

       190        200        210        220        230        240 
DELIFAAKQA WRNAPRCIGR IQWSNLQVFD ARDCKTAKEM FEYICRHIQY ATNNGNIRSA 

       250        260        270        280        290        300 
ITIFPQRTDG KHDFRVWNSQ LIRYAGYQMP DGSVIGDPAS VEFTKLCIEL GWKPKYGRFD 

       310        320        330        340        350        360 
VVPLILQANG QDPEIFEYPP EIILEVPMEH PKYEWFKELD LKWYALPAVA NMLLEVGGLE 

       370        380        390        400        410        420 
FTACPFNGWY MGTEIGVRDF CDVQRYNILK EVGRRMGLES NKLASLWKDR AVVEINVAVL 

       430        440        450        460        470        480 
HSFQKQNVTI MDHHSAAESF MKYMQNEYRV RGGCPADWVW IVPPMSGSIT PVFHQEMLNY 

       490        500        510        520        530        540 
VLTPFFYYQV DAWKTHIWHD ETRRPKKREI KLSILAKAVL LASLLLQKTM AARPKVTVIY 

       550        560        570        580        590        600 
ATETGKSETL ANSLCSLFSC AFNTKILCMD EYNISDLEKE TLLLVVTSTF GNGDSPNNGK 

       610        620        630        640        650        660 
TLKNSLLTLK LLRKNIRYAV FGLGSTMYPE FCAFAHAIDQ KLSQLGALQL TPVGEGDELN 

       670        680        690        700        710        720 
GQEEAFRTWA VTAFKTACDI FDIRGKNSIQ LPEIYTSDDS WNPKKHRIVY DSQTMDLTKA 

       730        740        750        760        770        780 
LSDIHGKNVI PMKLKFRQNL QSLKSSRVTI LVKLSCETNQ EVHYLPGEHI GISPGNQPEL 

       790        800        810        820        830        840 
VHGLIARVKD APPADQTIRL ETCTEGGYWA SEKKIPACTL SQALTYLLDI TTPPTQQLLK 

       850        860        870        880        890        900 
KLSQLVTAEG DKQRLEVLCH STEEYNKWKF YNRPNILEVL EEFPSAEVST AFLLTQLPLL 

       910        920        930        940        950        960 
KPRYYSVSSS CDMTPREIHL TVAVVNYRTR DGQGPLHHGV CSTWLNKIAL NETVPCFVRS 

       970        980        990       1000       1010       1020 
ADGFRLPKEP AKPCILIGPG TGIAPFRSFW QQRLYDLEKK GIKGGDMILL FGCRHPDMDH 

      1030       1040       1050       1060       1070       1080 
IYKEEVEEMK RKGVLKEVFT AYSRQPGQPK VYVQDILQNE LETKVCNILH KEEGHLYVCG 

      1090       1100       1110       1120       1130 
DVRMARDVAQ TLKRMLVKKL NHTEQQAEEY FFQLKSQKRY HEDIFGAVFP HEVKRI 

« Hide

References

[1]"Molecular cloning and expression of an avian macrophage nitric-oxide synthase cDNA and the analysis of the genomic 5'-flanking region."
Lin A.W., Chang C.C., McCormick C.C.
J. Biol. Chem. 271:11911-11919(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Proinflammatory agents, IL-8 and IL-10, upregulate inducible nitric oxide synthase expression and nitric oxide production in avian osteoclast-like cells."
Sunyer T., Rothe L., Jiang X., Osdoby P., Collin-Osdoby P.
J. Cell. Biochem. 60:469-483(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 171-472.
Tissue: Osteoclast.
[3]"Molecular cloning and expression of inducible nitric oxide synthase in chick embryonic ventricular myocytes."
Shimizu T., Kinugawa K., Sugishita Y., Sugishita K., Harada K., Matsui H., Kohmoto O., Serizawa T., Takahashi T.
Cardiovasc. Res. 38:405-413(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 646-986.
Tissue: Heart.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U46504 mRNA. Translation: AAC59886.1.
U34045 mRNA. Translation: AAB17499.1.
D85422 mRNA. Translation: BAA12817.1.
RefSeqNP_990292.1. NM_204961.1.
UniGeneGga.3327.

3D structure databases

ProteinModelPortalQ90703.
SMRQ90703. Positions 80-499, 700-1127.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ90703.
PRIDEQ90703.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID395807.
KEGGgga:395807.

Organism-specific databases

CTD4843.

Phylogenomic databases

eggNOGCOG4362.
HOGENOMHOG000220884.
HOVERGENHBG000159.
KOK13241.
PhylomeDBQ90703.

Family and domain databases

Gene3D1.20.990.10. 1 hit.
3.90.340.10. 1 hit.
InterProIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR004030. NO_synthase_oxygenase_dom.
IPR012144. NOS_euk.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PANTHERPTHR19384:SF5. PTHR19384:SF5. 1 hit.
PfamPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF000333. NOS. 1 hit.
PRINTSPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMSSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20815875.
PROQ90703.

Entry information

Entry nameNOS2_CHICK
AccessionPrimary (citable) accession number: Q90703
Secondary accession number(s): Q90677, Q90934
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families