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Q90703

- NOS2_CHICK

UniProt

Q90703 - NOS2_CHICK

Protein

Nitric oxide synthase, inducible

Gene

NOS2

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO may serve as both a paracrine and autocrine signal for modulating osteoclast bone resorption. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 By similarity.By similarity

    Catalytic activityi

    2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

    Cofactori

    Heme group.By similarity
    Binds 1 FAD.By similarity
    Binds 1 FMN.By similarity
    Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.By similarity

    Enzyme regulationi

    Not stimulated by calcium/calmodulin.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi107 – 1071ZincBy similarity
    Metal bindingi112 – 1121ZincBy similarity
    Metal bindingi197 – 1971Iron (heme axial ligand)By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi620 – 65132FMNPROSITE-ProRule annotationAdd
    BLAST
    Nucleotide bindingi764 – 77512FADBy similarityAdd
    BLAST
    Nucleotide bindingi900 – 91011FADBy similarityAdd
    BLAST
    Nucleotide bindingi975 – 99319NADPBy similarityAdd
    BLAST
    Nucleotide bindingi1073 – 108816NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: InterPro
    2. FMN binding Source: InterPro
    3. heme binding Source: InterPro
    4. iron ion binding Source: InterPro
    5. NADP binding Source: InterPro
    6. nitric-oxide synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. nitric oxide biosynthetic process Source: InterPro
    2. peptidyl-cysteine S-nitrosylation Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitric oxide synthase, inducible (EC:1.14.13.39)
    Alternative name(s):
    Inducible NO synthase
    Short name:
    Inducible NOS
    Short name:
    iNOS
    Macrophage NOS
    NOS type II
    Peptidyl-cysteine S-nitrosylase NOS2
    Gene namesi
    Name:NOS2
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11361136Nitric oxide synthase, induciblePRO_0000170938Add
    BLAST

    Proteomic databases

    PaxDbiQ90703.
    PRIDEiQ90703.

    Expressioni

    Inductioni

    By treatment with lipopolysaccharides (LPS) or cytokines, but not in osteoclasts where they are induced by calcium and PMA (phorbol 12-myristate 13-acetate).

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ90703.
    SMRiQ90703. Positions 80-499, 700-1127.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini536 – 674139Flavodoxin-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini727 – 967241FAD-binding FR-typePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni506 – 52621Calmodulin-bindingSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the NOS family.Curated
    Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
    Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG4362.
    HOGENOMiHOG000220884.
    HOVERGENiHBG000159.
    KOiK13241.
    PhylomeDBiQ90703.

    Family and domain databases

    Gene3Di1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    3.90.340.10. 1 hit.
    InterProiIPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR012144. NOS_euk.
    IPR004030. NOS_N.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view]
    PfamiPF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    PF02898. NO_synthase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000333. NOS. 1 hit.
    PRINTSiPR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMiSSF52218. SSF52218. 1 hit.
    SSF56512. SSF56512. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEiPS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    PS60001. NOS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q90703-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLCPWQFAFK PHAVKNQSSE EKDINNNVEK DVKVHSFVKD DAKLHSLSKK     50
    QMKMSPIITS AEKHPQNGIK ASNQISRCPR HVKVRNMENG SSLLDTLHLT 100
    AKEVINCRTR ACQGALMTPK GLVRSTRDGP VPPAELLPQA VDFVKQYYSS 150
    FKELKIEEHL ARLETVTKEI ETTGTYHLTK DELIFAAKQA WRNAPRCIGR 200
    IQWSNLQVFD ARDCKTAKEM FEYICRHIQY ATNNGNIRSA ITIFPQRTDG 250
    KHDFRVWNSQ LIRYAGYQMP DGSVIGDPAS VEFTKLCIEL GWKPKYGRFD 300
    VVPLILQANG QDPEIFEYPP EIILEVPMEH PKYEWFKELD LKWYALPAVA 350
    NMLLEVGGLE FTACPFNGWY MGTEIGVRDF CDVQRYNILK EVGRRMGLES 400
    NKLASLWKDR AVVEINVAVL HSFQKQNVTI MDHHSAAESF MKYMQNEYRV 450
    RGGCPADWVW IVPPMSGSIT PVFHQEMLNY VLTPFFYYQV DAWKTHIWHD 500
    ETRRPKKREI KLSILAKAVL LASLLLQKTM AARPKVTVIY ATETGKSETL 550
    ANSLCSLFSC AFNTKILCMD EYNISDLEKE TLLLVVTSTF GNGDSPNNGK 600
    TLKNSLLTLK LLRKNIRYAV FGLGSTMYPE FCAFAHAIDQ KLSQLGALQL 650
    TPVGEGDELN GQEEAFRTWA VTAFKTACDI FDIRGKNSIQ LPEIYTSDDS 700
    WNPKKHRIVY DSQTMDLTKA LSDIHGKNVI PMKLKFRQNL QSLKSSRVTI 750
    LVKLSCETNQ EVHYLPGEHI GISPGNQPEL VHGLIARVKD APPADQTIRL 800
    ETCTEGGYWA SEKKIPACTL SQALTYLLDI TTPPTQQLLK KLSQLVTAEG 850
    DKQRLEVLCH STEEYNKWKF YNRPNILEVL EEFPSAEVST AFLLTQLPLL 900
    KPRYYSVSSS CDMTPREIHL TVAVVNYRTR DGQGPLHHGV CSTWLNKIAL 950
    NETVPCFVRS ADGFRLPKEP AKPCILIGPG TGIAPFRSFW QQRLYDLEKK 1000
    GIKGGDMILL FGCRHPDMDH IYKEEVEEMK RKGVLKEVFT AYSRQPGQPK 1050
    VYVQDILQNE LETKVCNILH KEEGHLYVCG DVRMARDVAQ TLKRMLVKKL 1100
    NHTEQQAEEY FFQLKSQKRY HEDIFGAVFP HEVKRI 1136
    Length:1,136
    Mass (Da):129,649
    Last modified:November 1, 1997 - v1
    Checksum:iD5CB73AC7BA94B9D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti400 – 4001S → T in AAB17499. (PubMed:8707887)Curated
    Sequence conflicti873 – 8731R → S in BAA12817. (PubMed:9709401)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U46504 mRNA. Translation: AAC59886.1.
    U34045 mRNA. Translation: AAB17499.1.
    D85422 mRNA. Translation: BAA12817.1.
    RefSeqiNP_990292.1. NM_204961.1.
    UniGeneiGga.3327.

    Genome annotation databases

    GeneIDi395807.
    KEGGigga:395807.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U46504 mRNA. Translation: AAC59886.1 .
    U34045 mRNA. Translation: AAB17499.1 .
    D85422 mRNA. Translation: BAA12817.1 .
    RefSeqi NP_990292.1. NM_204961.1.
    UniGenei Gga.3327.

    3D structure databases

    ProteinModelPortali Q90703.
    SMRi Q90703. Positions 80-499, 700-1127.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q90703.
    PRIDEi Q90703.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 395807.
    KEGGi gga:395807.

    Organism-specific databases

    CTDi 4843.

    Phylogenomic databases

    eggNOGi COG4362.
    HOGENOMi HOG000220884.
    HOVERGENi HBG000159.
    KOi K13241.
    PhylomeDBi Q90703.

    Miscellaneous databases

    NextBioi 20815875.
    PROi Q90703.

    Family and domain databases

    Gene3Di 1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    3.90.340.10. 1 hit.
    InterProi IPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR012144. NOS_euk.
    IPR004030. NOS_N.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view ]
    Pfami PF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    PF02898. NO_synthase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000333. NOS. 1 hit.
    PRINTSi PR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMi SSF52218. SSF52218. 1 hit.
    SSF56512. SSF56512. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEi PS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    PS60001. NOS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and expression of an avian macrophage nitric-oxide synthase cDNA and the analysis of the genomic 5'-flanking region."
      Lin A.W., Chang C.C., McCormick C.C.
      J. Biol. Chem. 271:11911-11919(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Proinflammatory agents, IL-8 and IL-10, upregulate inducible nitric oxide synthase expression and nitric oxide production in avian osteoclast-like cells."
      Sunyer T., Rothe L., Jiang X., Osdoby P., Collin-Osdoby P.
      J. Cell. Biochem. 60:469-483(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 171-472.
      Tissue: Osteoclast.
    3. "Molecular cloning and expression of inducible nitric oxide synthase in chick embryonic ventricular myocytes."
      Shimizu T., Kinugawa K., Sugishita Y., Sugishita K., Harada K., Matsui H., Kohmoto O., Serizawa T., Takahashi T.
      Cardiovasc. Res. 38:405-413(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 646-986.
      Tissue: Heart.

    Entry informationi

    Entry nameiNOS2_CHICK
    AccessioniPrimary (citable) accession number: Q90703
    Secondary accession number(s): Q90677, Q90934
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3