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Q90703

- NOS2_CHICK

UniProt

Q90703 - NOS2_CHICK

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Protein
Nitric oxide synthase, inducible
Gene
NOS2
Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO may serve as both a paracrine and autocrine signal for modulating osteoclast bone resorption. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 By similarity.

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Heme group By similarity.
Binds 1 FAD By similarity.
Binds 1 FMN By similarity.
Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.

Enzyme regulationi

Not stimulated by calcium/calmodulin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi107 – 1071Zinc By similarity
Metal bindingi112 – 1121Zinc By similarity
Metal bindingi197 – 1971Iron (heme axial ligand) By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi620 – 65132FMN By similarity
Add
BLAST
Nucleotide bindingi764 – 77512FAD By similarity
Add
BLAST
Nucleotide bindingi900 – 91011FAD By similarity
Add
BLAST
Nucleotide bindingi975 – 99319NADP By similarity
Add
BLAST
Nucleotide bindingi1073 – 108816NADP By similarity
Add
BLAST

GO - Molecular functioni

  1. FMN binding Source: InterPro
  2. NADP binding Source: InterPro
  3. flavin adenine dinucleotide binding Source: InterPro
  4. heme binding Source: InterPro
  5. iron ion binding Source: InterPro
  6. nitric-oxide synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. nitric oxide biosynthetic process Source: InterPro
  2. peptidyl-cysteine S-nitrosylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase, inducible (EC:1.14.13.39)
Alternative name(s):
Inducible NO synthase
Short name:
Inducible NOS
Short name:
iNOS
Macrophage NOS
NOS type II
Peptidyl-cysteine S-nitrosylase NOS2
Gene namesi
Name:NOS2
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11361136Nitric oxide synthase, inducible
PRO_0000170938Add
BLAST

Proteomic databases

PaxDbiQ90703.
PRIDEiQ90703.

Expressioni

Inductioni

By treatment with lipopolysaccharides (LPS) or cytokines, but not in osteoclasts where they are induced by calcium and PMA (phorbol 12-myristate 13-acetate).

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ90703.
SMRiQ90703. Positions 80-499, 700-1127.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini536 – 674139Flavodoxin-like
Add
BLAST
Domaini727 – 967241FAD-binding FR-type
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni506 – 52621Calmodulin-binding Reviewed prediction
Add
BLAST

Sequence similaritiesi

Belongs to the NOS family.

Phylogenomic databases

eggNOGiCOG4362.
HOGENOMiHOG000220884.
HOVERGENiHBG000159.
KOiK13241.
PhylomeDBiQ90703.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
3.90.340.10. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000333. NOS. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q90703-1 [UniParc]FASTAAdd to Basket

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MLCPWQFAFK PHAVKNQSSE EKDINNNVEK DVKVHSFVKD DAKLHSLSKK     50
QMKMSPIITS AEKHPQNGIK ASNQISRCPR HVKVRNMENG SSLLDTLHLT 100
AKEVINCRTR ACQGALMTPK GLVRSTRDGP VPPAELLPQA VDFVKQYYSS 150
FKELKIEEHL ARLETVTKEI ETTGTYHLTK DELIFAAKQA WRNAPRCIGR 200
IQWSNLQVFD ARDCKTAKEM FEYICRHIQY ATNNGNIRSA ITIFPQRTDG 250
KHDFRVWNSQ LIRYAGYQMP DGSVIGDPAS VEFTKLCIEL GWKPKYGRFD 300
VVPLILQANG QDPEIFEYPP EIILEVPMEH PKYEWFKELD LKWYALPAVA 350
NMLLEVGGLE FTACPFNGWY MGTEIGVRDF CDVQRYNILK EVGRRMGLES 400
NKLASLWKDR AVVEINVAVL HSFQKQNVTI MDHHSAAESF MKYMQNEYRV 450
RGGCPADWVW IVPPMSGSIT PVFHQEMLNY VLTPFFYYQV DAWKTHIWHD 500
ETRRPKKREI KLSILAKAVL LASLLLQKTM AARPKVTVIY ATETGKSETL 550
ANSLCSLFSC AFNTKILCMD EYNISDLEKE TLLLVVTSTF GNGDSPNNGK 600
TLKNSLLTLK LLRKNIRYAV FGLGSTMYPE FCAFAHAIDQ KLSQLGALQL 650
TPVGEGDELN GQEEAFRTWA VTAFKTACDI FDIRGKNSIQ LPEIYTSDDS 700
WNPKKHRIVY DSQTMDLTKA LSDIHGKNVI PMKLKFRQNL QSLKSSRVTI 750
LVKLSCETNQ EVHYLPGEHI GISPGNQPEL VHGLIARVKD APPADQTIRL 800
ETCTEGGYWA SEKKIPACTL SQALTYLLDI TTPPTQQLLK KLSQLVTAEG 850
DKQRLEVLCH STEEYNKWKF YNRPNILEVL EEFPSAEVST AFLLTQLPLL 900
KPRYYSVSSS CDMTPREIHL TVAVVNYRTR DGQGPLHHGV CSTWLNKIAL 950
NETVPCFVRS ADGFRLPKEP AKPCILIGPG TGIAPFRSFW QQRLYDLEKK 1000
GIKGGDMILL FGCRHPDMDH IYKEEVEEMK RKGVLKEVFT AYSRQPGQPK 1050
VYVQDILQNE LETKVCNILH KEEGHLYVCG DVRMARDVAQ TLKRMLVKKL 1100
NHTEQQAEEY FFQLKSQKRY HEDIFGAVFP HEVKRI 1136
Length:1,136
Mass (Da):129,649
Last modified:November 1, 1997 - v1
Checksum:iD5CB73AC7BA94B9D
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti400 – 4001S → T in AAB17499. 1 Publication
Sequence conflicti873 – 8731R → S in BAA12817. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U46504 mRNA. Translation: AAC59886.1.
U34045 mRNA. Translation: AAB17499.1.
D85422 mRNA. Translation: BAA12817.1.
RefSeqiNP_990292.1. NM_204961.1.
UniGeneiGga.3327.

Genome annotation databases

GeneIDi395807.
KEGGigga:395807.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U46504 mRNA. Translation: AAC59886.1 .
U34045 mRNA. Translation: AAB17499.1 .
D85422 mRNA. Translation: BAA12817.1 .
RefSeqi NP_990292.1. NM_204961.1.
UniGenei Gga.3327.

3D structure databases

ProteinModelPortali Q90703.
SMRi Q90703. Positions 80-499, 700-1127.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q90703.
PRIDEi Q90703.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 395807.
KEGGi gga:395807.

Organism-specific databases

CTDi 4843.

Phylogenomic databases

eggNOGi COG4362.
HOGENOMi HOG000220884.
HOVERGENi HBG000159.
KOi K13241.
PhylomeDBi Q90703.

Miscellaneous databases

NextBioi 20815875.
PROi Q90703.

Family and domain databases

Gene3Di 1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
3.90.340.10. 1 hit.
InterProi IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000333. NOS. 1 hit.
PRINTSi PR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMi SSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEi PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and expression of an avian macrophage nitric-oxide synthase cDNA and the analysis of the genomic 5'-flanking region."
    Lin A.W., Chang C.C., McCormick C.C.
    J. Biol. Chem. 271:11911-11919(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Proinflammatory agents, IL-8 and IL-10, upregulate inducible nitric oxide synthase expression and nitric oxide production in avian osteoclast-like cells."
    Sunyer T., Rothe L., Jiang X., Osdoby P., Collin-Osdoby P.
    J. Cell. Biochem. 60:469-483(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 171-472.
    Tissue: Osteoclast.
  3. "Molecular cloning and expression of inducible nitric oxide synthase in chick embryonic ventricular myocytes."
    Shimizu T., Kinugawa K., Sugishita Y., Sugishita K., Harada K., Matsui H., Kohmoto O., Serizawa T., Takahashi T.
    Cardiovasc. Res. 38:405-413(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 646-986.
    Tissue: Heart.

Entry informationi

Entry nameiNOS2_CHICK
AccessioniPrimary (citable) accession number: Q90703
Secondary accession number(s): Q90677, Q90934
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: September 3, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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