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Protein

Nitric oxide synthase, inducible

Gene

NOS2

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO may serve as both a paracrine and autocrine signal for modulating osteoclast bone resorption. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 (By similarity).By similarity

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Protein has several cofactor binding sites:

Enzyme regulationi

Not stimulated by calcium/calmodulin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi107ZincBy similarity1
Metal bindingi112ZincBy similarity1
Metal bindingi197Iron (heme axial ligand)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi620 – 651FMNPROSITE-ProRule annotationAdd BLAST32
Nucleotide bindingi764 – 775FADBy similarityAdd BLAST12
Nucleotide bindingi900 – 910FADBy similarityAdd BLAST11
Nucleotide bindingi975 – 993NADPBy similarityAdd BLAST19
Nucleotide bindingi1073 – 1088NADPBy similarityAdd BLAST16

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCalmodulin-binding, Oxidoreductase
LigandFAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase, inducible (EC:1.14.13.39)
Alternative name(s):
Inducible NO synthase
Short name:
Inducible NOS
Short name:
iNOS
Macrophage NOS
NOS type II
Peptidyl-cysteine S-nitrosylase NOS2
Gene namesi
Name:NOS2
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001709381 – 1136Nitric oxide synthase, inducibleAdd BLAST1136

Proteomic databases

PaxDbiQ90703

Expressioni

Inductioni

By treatment with lipopolysaccharides (LPS) or cytokines, but not in osteoclasts where they are induced by calcium and PMA (phorbol 12-myristate 13-acetate).

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000034026

Structurei

3D structure databases

ProteinModelPortaliQ90703
SMRiQ90703
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini536 – 674Flavodoxin-likePROSITE-ProRule annotationAdd BLAST139
Domaini727 – 967FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST241

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni506 – 526Calmodulin-bindingSequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the NOS family.Curated

Phylogenomic databases

eggNOGiKOG1158 Eukaryota
COG0369 LUCA
COG4362 LUCA
HOGENOMiHOG000220884
HOVERGENiHBG000159
InParanoidiQ90703
KOiK13241
PhylomeDBiQ90703

Family and domain databases

Gene3Di1.20.990.10, 1 hit
3.40.50.360, 1 hit
InterProiView protein in InterPro
IPR003097 FAD-binding_1
IPR017927 Fd_Rdtase_FAD-bd
IPR001094 Flavdoxin-like
IPR008254 Flavodoxin/NO_synth
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR029039 Flavoprotein-like_sf
IPR023173 NADPH_Cyt_P450_Rdtase_alpha
IPR012144 NOS_euk
IPR004030 NOS_N
IPR036119 NOS_N_sf
IPR001433 OxRdtase_FAD/NAD-bd
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00667 FAD_binding_1, 1 hit
PF00258 Flavodoxin_1, 1 hit
PF00175 NAD_binding_1, 1 hit
PF02898 NO_synthase, 1 hit
PIRSFiPIRSF000333 NOS, 1 hit
PRINTSiPR00369 FLAVODOXIN
PR00371 FPNCR
SUPFAMiSSF52218 SSF52218, 1 hit
SSF56512 SSF56512, 1 hit
SSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit
PS50902 FLAVODOXIN_LIKE, 1 hit
PS60001 NOS, 1 hit

Sequencei

Sequence statusi: Complete.

Q90703-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLCPWQFAFK PHAVKNQSSE EKDINNNVEK DVKVHSFVKD DAKLHSLSKK
60 70 80 90 100
QMKMSPIITS AEKHPQNGIK ASNQISRCPR HVKVRNMENG SSLLDTLHLT
110 120 130 140 150
AKEVINCRTR ACQGALMTPK GLVRSTRDGP VPPAELLPQA VDFVKQYYSS
160 170 180 190 200
FKELKIEEHL ARLETVTKEI ETTGTYHLTK DELIFAAKQA WRNAPRCIGR
210 220 230 240 250
IQWSNLQVFD ARDCKTAKEM FEYICRHIQY ATNNGNIRSA ITIFPQRTDG
260 270 280 290 300
KHDFRVWNSQ LIRYAGYQMP DGSVIGDPAS VEFTKLCIEL GWKPKYGRFD
310 320 330 340 350
VVPLILQANG QDPEIFEYPP EIILEVPMEH PKYEWFKELD LKWYALPAVA
360 370 380 390 400
NMLLEVGGLE FTACPFNGWY MGTEIGVRDF CDVQRYNILK EVGRRMGLES
410 420 430 440 450
NKLASLWKDR AVVEINVAVL HSFQKQNVTI MDHHSAAESF MKYMQNEYRV
460 470 480 490 500
RGGCPADWVW IVPPMSGSIT PVFHQEMLNY VLTPFFYYQV DAWKTHIWHD
510 520 530 540 550
ETRRPKKREI KLSILAKAVL LASLLLQKTM AARPKVTVIY ATETGKSETL
560 570 580 590 600
ANSLCSLFSC AFNTKILCMD EYNISDLEKE TLLLVVTSTF GNGDSPNNGK
610 620 630 640 650
TLKNSLLTLK LLRKNIRYAV FGLGSTMYPE FCAFAHAIDQ KLSQLGALQL
660 670 680 690 700
TPVGEGDELN GQEEAFRTWA VTAFKTACDI FDIRGKNSIQ LPEIYTSDDS
710 720 730 740 750
WNPKKHRIVY DSQTMDLTKA LSDIHGKNVI PMKLKFRQNL QSLKSSRVTI
760 770 780 790 800
LVKLSCETNQ EVHYLPGEHI GISPGNQPEL VHGLIARVKD APPADQTIRL
810 820 830 840 850
ETCTEGGYWA SEKKIPACTL SQALTYLLDI TTPPTQQLLK KLSQLVTAEG
860 870 880 890 900
DKQRLEVLCH STEEYNKWKF YNRPNILEVL EEFPSAEVST AFLLTQLPLL
910 920 930 940 950
KPRYYSVSSS CDMTPREIHL TVAVVNYRTR DGQGPLHHGV CSTWLNKIAL
960 970 980 990 1000
NETVPCFVRS ADGFRLPKEP AKPCILIGPG TGIAPFRSFW QQRLYDLEKK
1010 1020 1030 1040 1050
GIKGGDMILL FGCRHPDMDH IYKEEVEEMK RKGVLKEVFT AYSRQPGQPK
1060 1070 1080 1090 1100
VYVQDILQNE LETKVCNILH KEEGHLYVCG DVRMARDVAQ TLKRMLVKKL
1110 1120 1130
NHTEQQAEEY FFQLKSQKRY HEDIFGAVFP HEVKRI
Length:1,136
Mass (Da):129,649
Last modified:November 1, 1997 - v1
Checksum:iD5CB73AC7BA94B9D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti400S → T in AAB17499 (PubMed:8707887).Curated1
Sequence conflicti873R → S in BAA12817 (PubMed:9709401).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U46504 mRNA Translation: AAC59886.1
U34045 mRNA Translation: AAB17499.1
D85422 mRNA Translation: BAA12817.1
RefSeqiNP_990292.1, NM_204961.1
UniGeneiGga.3327

Genome annotation databases

GeneIDi395807
KEGGigga:395807

Similar proteinsi

Entry informationi

Entry nameiNOS2_CHICK
AccessioniPrimary (citable) accession number: Q90703
Secondary accession number(s): Q90677, Q90934
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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