Reviewed, UniProtKB/Swiss-Prot Q90628 (TRY2_CHICK)

Names and origin

Protein names	Recommended name:     Trypsin I-P38     EC=3.4.21.4
Organism	Gallus gallus (Chicken)
Taxonomic identifier	9031 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted › extracellular space.
Tissue specificity	High levels are seen in the pancreas while lower levels are found in the liver, spleen and thymus.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15	By similarity
	Propeptide	16 – 25	10	Activation peptide By similarity		PRO_0000028221
	Chain	26 – 248	223	Trypsin I-P38		PRO_0000028222
Regions
	Domain	26 – 246	221	Peptidase S1
Sites
	Active site	65	1	Charge relay system By similarity
	Active site	109	1	Charge relay system By similarity
	Active site	202	1	Charge relay system By similarity
	Metal binding	77	1	Calcium By similarity
	Metal binding	79	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	87	1	Calcium By similarity
	Site	196	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	32 ↔ 162		By similarity
	Disulfide bond	50 ↔ 66		By similarity
	Disulfide bond	134 ↔ 235		By similarity
	Disulfide bond	141 ↔ 208		By similarity
	Disulfide bond	173 ↔ 187		By similarity
	Disulfide bond	198 ↔ 222		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q90628-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 78B79DD6FE15F0CE	FASTA	248	26,088
	10 20 30 40 50 60 MKFLVLVAFL GVAVAFPISD EDDDKIVGGY SCARSAAPYQ VSLNSGYHFC GGSLISSQWV 70 80 90 100 110 120 LSAAHCYKSS IQVKLGEYNL AAQDGSEQTI SSSKVIRHSG YNANTLNNDI MLIKLSKAAT 130 140 150 160 170 180 LNSYVNTVPL PTSCVTAGTT CLISGWGNTL SSGSLYPDVL QCLNAPVLSS SQCSSAYPGR 190 200 210 220 230 240 ITSNMICIGY LNGGKDSCQG DSGGPVVCNG QLQGFVSWGI GCAQKGYPGV YTKVCNYVSW IKTTMSSN

References

[1]	"Isolation and characterization of the chicken trypsinogen gene family." Wang K., Gan L., Lee I., Hood L.E. Biochem. J. 307:471-479(1995) [PubMed: 7733885] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pancreas.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U15156 mRNA. Translation: AAA79913.1.
IPI	IPI00571166.
PIR	S55067.
UniGene	Gga.4442
3D structure databases
SMR	Q90628. Positions 26-248.
ModBase	Search...
Genome annotation databases
Ensembl	ENSGALT00000016631; ENSGALP00000016612; ENSGALG00000010231; Gallus gallus. [Genome view] ENSGALT00000016632; ENSGALP00000016613; ENSGALG00000010231; Gallus gallus. [Genome view]
Phylogenomic databases
HOVERGEN	Q90628.
InParanoid	Q90628.
Enzyme and pathway databases
BRENDA	3.4.21.4. 4.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. IPR009003. Ser/Cys_Pept_Trypsin-like. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRY2_CHICK

Accession

Primary (citable) accession number: Q90628

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	December 15, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)