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Protein

Protein phosphatase 1 regulatory subunit 12A

Gene

PPP1R12A

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates myosin phosphatase activity.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1 regulatory subunit 12A
Alternative name(s):
130 kDa myosin-binding subunit of smooth muscle myosin phosphatase
Myosin phosphatase-targeting subunit 1
Short name:
Myosin phosphatase target subunit 1
PP1M subunit M110
Protein phosphatase myosin-binding subunit
Gene namesi
Name:PPP1R12A
Synonyms:MBS, MYPT1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

  • Cytoplasm By similarity

  • Note: Along actomyosin filaments and stress fibers.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi695T → A: Reduces phosphorylation. Reduces phosphorylation on serine and threonine residues by 80%; when associated with A-850. 1 Publication1
Mutagenesisi850T → A: Reduces phosphorylation. Reduces phosphorylation on serine and threonine residues by 80%; when associated with A-695. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003555561 – 1004Protein phosphatase 1 regulatory subunit 12AAdd BLAST1004

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei695Phosphothreonine; by ROCK22 Publications1
Modified residuei850Phosphothreonine; by ROCK22 Publications1

Post-translational modificationi

Phosphorylated by CIT (Rho-associated kinase) and by ROCK2 on serine and threonine residues. Phosphorylation at Thr-695 leads to inhibition of myosin phosphatase activity. Phosphorylation at Thr-850 abolishes myosin binding. May be phosphorylated at Thr-695 by DMPK; may inhibit the myosin phosphatase activity.3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ90623.
PRIDEiQ90623.

PTM databases

iPTMnetiQ90623.

Expressioni

Tissue specificityi

Detected in brain, lung, aorta, heart, gizzard, stomach, oviduct, spleen, kidney and small intestine.1 Publication

Interactioni

Subunit structurei

PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits (By similarity). PPP1R12A mediates binding to myosin.By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi676290. 1 interactor.
IntActiQ90623. 1 interactor.
STRINGi9031.ENSGALP00000016789.

Structurei

Secondary structure

11004
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 19Combined sources16
Turni20 – 22Combined sources3
Helixi40 – 50Combined sources11
Helixi53 – 62Combined sources10
Helixi76 – 82Combined sources7
Helixi86 – 94Combined sources9
Helixi109 – 116Combined sources8
Helixi119 – 127Combined sources9
Helixi142 – 145Combined sources4
Helixi149 – 162Combined sources14
Helixi166 – 187Combined sources22
Turni196 – 198Combined sources3
Helixi202 – 209Combined sources8
Helixi212 – 219Combined sources8
Turni220 – 222Combined sources3
Helixi235 – 241Combined sources7
Helixi245 – 253Combined sources9
Turni268 – 270Combined sources3
Helixi275 – 277Combined sources3
Helixi278 – 286Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S70X-ray2.70B1-299[»]
DisProtiDP00218.
ProteinModelPortaliQ90623.
SMRiQ90623.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ90623.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati39 – 68ANK 1Add BLAST30
Repeati72 – 101ANK 2Add BLAST30
Repeati105 – 134ANK 3Add BLAST30
Repeati138 – 164ANK 4Add BLAST27
Repeati198 – 227ANK 5Add BLAST30
Repeati231 – 260ANK 6Add BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni35 – 38Important for interaction with PPP1CB4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi343 – 370Asp/Glu-richAdd BLAST28
Compositional biasi514 – 660Ser/Thr-richAdd BLAST147
Compositional biasi720 – 751Asp/Glu/Lys-richAdd BLAST32
Compositional biasi771 – 811Ser-richAdd BLAST41
Compositional biasi771 – 793Ser/Thr-richAdd BLAST23

Sequence similaritiesi

Contains 6 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0505. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00760000119141.
HOGENOMiHOG000290648.
HOVERGENiHBG052561.
InParanoidiQ90623.
KOiK06270.
PhylomeDBiQ90623.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR017401. MYPT1/MYPT2_chordates.
IPR031775. PRKG1_interact.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
PF15898. PRKG1_interact. 1 hit.
[Graphical view]
PIRSFiPIRSF038141. PP1_12ABC_vert. 1 hit.
SMARTiSM00248. ANK. 6 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q90623-1) [UniParc]FASTAAdd to basket
Also known as: M133

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKMADAKQKR NEQLKRWIGS ETDLEPPVVK RKKTKVKFDD GAVFLAACSS
60 70 80 90 100
GDTEEVLRLL ERGADINYAN VDGLTALHQA CIDDNVDMVK FLVENGANIN
110 120 130 140 150
QPDNEGWIPL HAAASCGYLD IAEYLISQGA HVGAVNSEGD TPLDIAEEEA
160 170 180 190 200
MEELLQNEVN RQGVDIEAAR KEEERIMLRD ARQWLNSGHI NDVRHAKSGG
210 220 230 240 250
TALHVAAAKG YTEVLKLLIQ ARYDVNIKDY DGWTPLHAAA HWGKEEACRI
260 270 280 290 300
LVENLCDMEA VNKVGQTAFD VADEDILGYL EELQKKQNLL HSEKREKKSP
310 320 330 340 350
LIESTANLDN NQTQKTFKNK ETLIMEQEKN ASSIESLEHE KADEEEEGKK
360 370 380 390 400
DESSCSSEEE EDDDSESEAE TDKAKTLANA NTTSTQSASM TAPSVAGGQG
410 420 430 440 450
TPTSPLKKFP TSTTKVSPKE EERKDESPAS WRLGLRKTGS YGALAEITAS
460 470 480 490 500
KEAQKEKDSA GVIRSASSPR LSSSLDNKEK EKDGKGTRLA YVAPTIPRRL
510 520 530 540 550
ASTSDIDEKE NRDSSASSIR SGSSYARRKW EEDVKKNSLN EGPTSLNTSY
560 570 580 590 600
QRSGSFGRRQ DDLVSSNVPS TASTVTSSAG LQKTLPASAN TTTKSTTGST
610 620 630 640 650
SAGVQSSTSN RLWAEDSTEK EKDSVPTAVT VPVAPSVVNA AATTTAMTTA
660 670 680 690 700
TSGTVSSTSE VRERRRSYLT PVRDEESESQ RKARSRQARQ SRRSTQGVTL
710 720 730 740 750
TDLQEAEKTI GRSRSTRTRE QENEEKEKEE KEKQDKEKQE EKKESETKDD
760 770 780 790 800
DYRQRYSRTV EEPYHRYRPT STSTSTSSTS SLSTSTSSLS SSSQLNRPNS
810 820 830 840 850
LIGITSAYSR SGTKESEREG GKKEEEKEED KSQPKSIRER RRPREKRRST
860 870 880 890 900
GVSFWTQDSD ENEQEHQSDS EEGTNKKETQ SDSLSRYDTG SLSVSSGDRY
910 920 930 940 950
DSAQGRSGSQ SYLEDRKPYC SRLEKEDSTD FKKLYEQILA ENEKLKAQLH
960 970 980 990 1000
DTNMELTDLK LQLEKTTQRQ ERFADRSLLE MEKRVSGKSQ YLLGGKKSSR

KKDI
Length:1,004
Mass (Da):111,606
Last modified:November 1, 1996 - v1
Checksum:i7ED870DB87046929
GO
Isoform 2 (identifier: Q90623-2) [UniParc]FASTAAdd to basket
Also known as: M130

The sequence of this isoform differs from the canonical sequence as follows:
     512-552: Missing.

Show »
Length:963
Mass (Da):106,975
Checksum:i819D017491D02DB4
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_035912512 – 552Missing in isoform 2. 1 PublicationAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37985 mRNA. Translation: BAA07201.1.
D37986 mRNA. Translation: BAA07202.1.
S74623 mRNA. Translation: AAB32730.1.
PIRiA55142.
RefSeqiNP_990454.1. NM_205123.1. [Q90623-1]
XP_015133641.1. XM_015278155.1. [Q90623-1]
XP_015133841.1. XM_015278355.1. [Q90623-2]
UniGeneiGga.3159.

Genome annotation databases

EnsembliENSGALT00000065339; ENSGALP00000055263; ENSGALG00000010325. [Q90623-1]
GeneIDi396020.
KEGGigga:396020.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37985 mRNA. Translation: BAA07201.1.
D37986 mRNA. Translation: BAA07202.1.
S74623 mRNA. Translation: AAB32730.1.
PIRiA55142.
RefSeqiNP_990454.1. NM_205123.1. [Q90623-1]
XP_015133641.1. XM_015278155.1. [Q90623-1]
XP_015133841.1. XM_015278355.1. [Q90623-2]
UniGeneiGga.3159.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S70X-ray2.70B1-299[»]
DisProtiDP00218.
ProteinModelPortaliQ90623.
SMRiQ90623.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi676290. 1 interactor.
IntActiQ90623. 1 interactor.
STRINGi9031.ENSGALP00000016789.

PTM databases

iPTMnetiQ90623.

Proteomic databases

PaxDbiQ90623.
PRIDEiQ90623.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000065339; ENSGALP00000055263; ENSGALG00000010325. [Q90623-1]
GeneIDi396020.
KEGGigga:396020.

Organism-specific databases

CTDi4659.

Phylogenomic databases

eggNOGiKOG0505. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00760000119141.
HOGENOMiHOG000290648.
HOVERGENiHBG052561.
InParanoidiQ90623.
KOiK06270.
PhylomeDBiQ90623.

Miscellaneous databases

EvolutionaryTraceiQ90623.
PROiQ90623.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR017401. MYPT1/MYPT2_chordates.
IPR031775. PRKG1_interact.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
PF15898. PRKG1_interact. 1 hit.
[Graphical view]
PIRSFiPIRSF038141. PP1_12ABC_vert. 1 hit.
SMARTiSM00248. ANK. 6 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMYPT1_CHICK
AccessioniPrimary (citable) accession number: Q90623
Secondary accession number(s): Q10727, Q90624
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.