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Protein

Lysosome-associated membrane glycoprotein 2

Gene

LAMP2

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction (By similarity).By similarity

GO - Biological processi

  1. protein stabilization Source: Ensembl
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosome-associated membrane glycoprotein 2
Short name:
LAMP-2
Short name:
Lysosome-associated membrane protein 2
Gene namesi
Name:LAMP2
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Chromosome 4

Subcellular locationi

Cell membrane By similarity; Single-pass type I membrane protein PROSITE-ProRule annotation. Endosome membrane By similarity; Single-pass type I membrane protein PROSITE-ProRule annotation. Lysosome membrane PROSITE-ProRule annotation; Single-pass type I membrane protein PROSITE-ProRule annotation
Note: This protein shuttles between lysosomes, endosomes, and the plasma membrane.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini28 – 389362LumenalSequence AnalysisAdd
BLAST
Transmembranei390 – 41425HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini415 – 42511CytoplasmicPROSITE-ProRule annotationAdd
BLAST

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. integral component of membrane Source: UniProtKB-KW
  3. late endosome membrane Source: Ensembl
  4. lysosomal membrane Source: UniProtKB-SubCell
  5. phagocytic vesicle membrane Source: Ensembl
  6. plasma membrane Source: UniProtKB-SubCell
  7. platelet dense granule membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence AnalysisAdd
BLAST
Chaini28 – 425398Lysosome-associated membrane glycoprotein 2PRO_0000017114Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi37 – 371N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi40 ↔ 78PROSITE-ProRule annotation
Glycosylationi56 – 561N-linked (GlcNAc...)Sequence Analysis
Glycosylationi62 – 621N-linked (GlcNAc...)Sequence Analysis
Glycosylationi74 – 741N-linked (GlcNAc...)Sequence Analysis
Glycosylationi100 – 1001N-linked (GlcNAc...)Sequence Analysis
Glycosylationi105 – 1051N-linked (GlcNAc...)Sequence Analysis
Glycosylationi120 – 1201N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi153 ↔ 189PROSITE-ProRule annotation
Glycosylationi163 – 1631N-linked (GlcNAc...)Sequence Analysis
Glycosylationi170 – 1701N-linked (GlcNAc...)Sequence Analysis
Glycosylationi179 – 1791N-linked (GlcNAc...)Sequence Analysis
Glycosylationi206 – 2061N-linked (GlcNAc...)Sequence Analysis
Glycosylationi232 – 2321N-linked (GlcNAc...)Sequence Analysis
Glycosylationi239 – 2391N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi242 ↔ 274PROSITE-ProRule annotation
Glycosylationi252 – 2521N-linked (GlcNAc...)Sequence Analysis
Glycosylationi276 – 2761N-linked (GlcNAc...)Sequence Analysis
Glycosylationi287 – 2871N-linked (GlcNAc...)Sequence Analysis
Glycosylationi298 – 2981N-linked (GlcNAc...)Sequence Analysis
Glycosylationi312 – 3121N-linked (GlcNAc...)Sequence Analysis
Glycosylationi320 – 3201N-linked (GlcNAc...)Sequence Analysis
Glycosylationi331 – 3311N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi345 ↔ 382PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ90617.

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000013946.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni28 – 192165First lumenal domainAdd
BLAST
Regioni193 – 23846HingeAdd
BLAST
Regioni239 – 390152Second lumenal domainAdd
BLAST

Sequence similaritiesi

Belongs to the LAMP family.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG301998.
GeneTreeiENSGT00530000063068.
HOGENOMiHOG000230942.
HOVERGENiHBG052303.
InParanoidiQ90617.
KOiK06528.
OMAiAEECFAD.
OrthoDBiEOG7ZD1VH.
PhylomeDBiQ90617.
TreeFamiTF316339.

Family and domain databases

InterProiIPR018134. LAMP_CS.
IPR002000. Lysosome-assoc_membr_glycop.
[Graphical view]
PANTHERiPTHR11506. PTHR11506. 1 hit.
PfamiPF01299. Lamp. 1 hit.
[Graphical view]
PRINTSiPR00336. LYSASSOCTDMP.
PROSITEiPS00310. LAMP_1. 1 hit.
PS51407. LAMP_3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform LAMP-2B (identifier: Q90617-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPPRCPAGL ALLLLLLGAC GFFQSYAVEV DVKDASNFTC LYAQWMMKFL
60 70 80 90 100
IKYETNSSDY KNASLDLTST VTHNGSICGS DTQAALLAVQ FGDGHSWSIN
110 120 130 140 150
FTKNNETYRA EFITFTYNTN DTAVFPDARR QGPVTIVVKD AMHPIQLNNV
160 170 180 190 200
FVCHHTTSLE AENVTQIFWN VTMQPFVQNG TISKKESRCY ADTPTAAPTV
210 220 230 240 250
LPTVANVTTA STTISPAPTT APKPAENPVT GNYSLKTGNK TCLLATVGLQ
260 270 280 290 300
LNISQDKPLL INIDPKTTHA DGTCGNTSAT LKLNDGNRTL IDFTFIVNAS
310 320 330 340 350
ASVQKFYLRE VNVTLLNYQN GSVILSADNN NLSKWDASLG NSYMCRKEQT
360 370 380 390 400
LEINENLQVH TFNLWVQPFL VKENKFSIAE ECFADSDLNF LIPVAVGMAL
410 420
GFLIILVFIS YIIGRRKSRT GYQSV
Length:425
Mass (Da):46,715
Last modified:November 1, 1996 - v1
Checksum:iAC16730A98FDB359
GO
Isoform LAMP-2A (identifier: Q90617-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     381-425: ECFADSDLNF...RKSRTGYQSV → DCSPEVDYFI...KHHNTGYEQF

Show »
Length:425
Mass (Da):46,641
Checksum:i666DC3134A46F18E
GO
Isoform LAMP-2C (identifier: Q90617-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     380-425: EECFADSDLN...RKSRTGYQSV → QECSLDDDTI...RKSYAGYQTL

Show »
Length:424
Mass (Da):46,411
Checksum:i94CA9409BF78F11C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti261 – 2611I → L in AAA99539 (PubMed:7546292).Curated
Sequence conflicti261 – 2611I → L in AAA99541 (PubMed:7546292).Curated
Sequence conflicti277 – 2771T → A in AAA99539 (PubMed:7546292).Curated
Sequence conflicti277 – 2771T → A in AAA99541 (PubMed:7546292).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei380 – 42546EECFA…GYQSV → QECSLDDDTILIPIVVGAAL AGLIVIIVIAYIIGRRKSYA GYQTL in isoform LAMP-2C. 1 PublicationVSP_003047Add
BLAST
Alternative sequencei381 – 42545ECFAD…GYQSV → DCSPEVDYFIVPIAVGAALG GLVVLVIMAYFLGHKKHHNT GYEQF in isoform LAMP-2A. 1 PublicationVSP_003048Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10547 mRNA. Translation: AAA99540.1.
U10546 mRNA. Translation: AAA99539.1.
U10548 mRNA. Translation: AAA99541.1.
RefSeqiNP_001001749.1. NM_001001749.1. [Q90617-1]
UniGeneiGga.2942.

Genome annotation databases

EnsembliENSGALT00000013962; ENSGALP00000013946; ENSGALG00000008572. [Q90617-1]
GeneIDi414800.
KEGGigga:414800.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10547 mRNA. Translation: AAA99540.1.
U10546 mRNA. Translation: AAA99539.1.
U10548 mRNA. Translation: AAA99541.1.
RefSeqiNP_001001749.1. NM_001001749.1. [Q90617-1]
UniGeneiGga.2942.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000013946.

Proteomic databases

PaxDbiQ90617.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000013962; ENSGALP00000013946; ENSGALG00000008572. [Q90617-1]
GeneIDi414800.
KEGGigga:414800.

Organism-specific databases

CTDi3920.

Phylogenomic databases

eggNOGiNOG301998.
GeneTreeiENSGT00530000063068.
HOGENOMiHOG000230942.
HOVERGENiHBG052303.
InParanoidiQ90617.
KOiK06528.
OMAiAEECFAD.
OrthoDBiEOG7ZD1VH.
PhylomeDBiQ90617.
TreeFamiTF316339.

Miscellaneous databases

NextBioi20818724.
PROiQ90617.

Family and domain databases

InterProiIPR018134. LAMP_CS.
IPR002000. Lysosome-assoc_membr_glycop.
[Graphical view]
PANTHERiPTHR11506. PTHR11506. 1 hit.
PfamiPF01299. Lamp. 1 hit.
[Graphical view]
PRINTSiPR00336. LYSASSOCTDMP.
PROSITEiPS00310. LAMP_1. 1 hit.
PS51407. LAMP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The family of LAMP-2 proteins arises by alternative splicing from a single gene: characterization of the avian LAMP-2 gene and identification of mammalian homologs of LAMP-2b and LAMP-2c."
    Gough N.R., Hatem C.L., Fambrough D.M.
    DNA Cell Biol. 14:863-867(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LAMP-2A; LAMP-2B AND LAMP-2C).
    Strain: White leghorn.
    Tissue: Brain.

Entry informationi

Entry nameiLAMP2_CHICK
AccessioniPrimary (citable) accession number: Q90617
Secondary accession number(s): Q90616, Q90618
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: February 4, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.