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Protein

Lysosome-associated membrane glycoprotein 2

Gene

LAMP2

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live. Functions by binding target proteins, such as GAPDH and MLLT11, and targeting them for lysosomal degradation (By similarity). Plays a role in lysosomal protein degradation in response to starvation (By similarity). Required for the fusion of autophagosomes with lysosomes during autophagy. Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes. Required for normal degradation of the contents of autophagosomes. Required for efficient MHCII-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHCII subunits. Is not required for efficient MHCII-mediated presentation of endogenous antigens (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAutophagy

Enzyme and pathway databases

ReactomeiR-GGA-114608. Platelet degranulation.
R-GGA-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosome-associated membrane glycoprotein 2
Short name:
LAMP-2
Short name:
Lysosome-associated membrane protein 2
Gene namesi
Name:LAMP2
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 4

Subcellular locationi

  • Cell membrane By similarity; Single-pass type I membrane protein PROSITE-ProRule annotation
  • Endosome membrane By similarity; Single-pass type I membrane protein PROSITE-ProRule annotation
  • Lysosome membrane PROSITE-ProRule annotation; Single-pass type I membrane protein PROSITE-ProRule annotation
  • Cytoplasmic vesicleautophagosome membrane By similarity

  • Note: This protein shuttles between lysosomes, endosomes, and the plasma membrane.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini28 – 389LumenalSequence analysisAdd BLAST362
Transmembranei390 – 414HelicalPROSITE-ProRule annotationAdd BLAST25
Topological domaini415 – 425CytoplasmicPROSITE-ProRule annotationAdd BLAST11

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000001711428 – 425Lysosome-associated membrane glycoprotein 2Add BLAST398

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi37N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi40 ↔ 78PROSITE-ProRule annotation
Glycosylationi56N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi62N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi74N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi100N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi105N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi120N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi153 ↔ 189PROSITE-ProRule annotation
Glycosylationi163N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi170N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi179N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi206N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi232N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi239N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi242 ↔ 274PROSITE-ProRule annotation
Glycosylationi252N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi276N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi287N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi298N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi312N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi320N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi331N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi345 ↔ 382PROSITE-ProRule annotation

Post-translational modificationi

Extensively N-glycosylated. Contains a minor proportion of O-linked glycans.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ90617.

Expressioni

Gene expression databases

BgeeiENSGALG00000008572.

Interactioni

Subunit structurei

Monomer. Forms large homooligomers. Interacts (via its cytoplasmic region) with HSPA8. Interacts with HSP90 in the lysosome lumen; this enhances LAMP2 stability (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000013946.

Structurei

3D structure databases

SMRiQ90617.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni28 – 192First lumenal domainAdd BLAST165
Regioni193 – 238HingeAdd BLAST46
Regioni239 – 390Second lumenal domainAdd BLAST152
Regioni416 – 419Important for binding and subsequent lysosomal degradation of target proteinsBy similarity4

Sequence similaritiesi

Belongs to the LAMP family.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4818. Eukaryota.
ENOG410XQ96. LUCA.
GeneTreeiENSGT00530000063068.
HOGENOMiHOG000230942.
HOVERGENiHBG052303.
InParanoidiQ90617.
KOiK06528.
OMAiPIIHTTV.
OrthoDBiEOG091G09EV.
PhylomeDBiQ90617.
TreeFamiTF316339.

Family and domain databases

InterProiView protein in InterPro
IPR018134. LAMP_CS.
IPR002000. Lysosome-assoc_membr_glycop.
PANTHERiPTHR11506. PTHR11506. 1 hit.
PfamiView protein in Pfam
PF01299. Lamp. 1 hit.
PRINTSiPR00336. LYSASSOCTDMP.
PROSITEiView protein in PROSITE
PS00310. LAMP_1. 1 hit.
PS51407. LAMP_3. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform LAMP-2B (identifier: Q90617-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPPRCPAGL ALLLLLLGAC GFFQSYAVEV DVKDASNFTC LYAQWMMKFL
60 70 80 90 100
IKYETNSSDY KNASLDLTST VTHNGSICGS DTQAALLAVQ FGDGHSWSIN
110 120 130 140 150
FTKNNETYRA EFITFTYNTN DTAVFPDARR QGPVTIVVKD AMHPIQLNNV
160 170 180 190 200
FVCHHTTSLE AENVTQIFWN VTMQPFVQNG TISKKESRCY ADTPTAAPTV
210 220 230 240 250
LPTVANVTTA STTISPAPTT APKPAENPVT GNYSLKTGNK TCLLATVGLQ
260 270 280 290 300
LNISQDKPLL INIDPKTTHA DGTCGNTSAT LKLNDGNRTL IDFTFIVNAS
310 320 330 340 350
ASVQKFYLRE VNVTLLNYQN GSVILSADNN NLSKWDASLG NSYMCRKEQT
360 370 380 390 400
LEINENLQVH TFNLWVQPFL VKENKFSIAE ECFADSDLNF LIPVAVGMAL
410 420
GFLIILVFIS YIIGRRKSRT GYQSV
Length:425
Mass (Da):46,715
Last modified:November 1, 1996 - v1
Checksum:iAC16730A98FDB359
GO
Isoform LAMP-2A (identifier: Q90617-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     381-425: ECFADSDLNF...RKSRTGYQSV → DCSPEVDYFI...KHHNTGYEQF

Show »
Length:425
Mass (Da):46,641
Checksum:i666DC3134A46F18E
GO
Isoform LAMP-2C (identifier: Q90617-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     380-425: EECFADSDLN...RKSRTGYQSV → QECSLDDDTI...RKSYAGYQTL

Show »
Length:424
Mass (Da):46,411
Checksum:i94CA9409BF78F11C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti261I → L in AAA99539 (PubMed:7546292).Curated1
Sequence conflicti261I → L in AAA99541 (PubMed:7546292).Curated1
Sequence conflicti277T → A in AAA99539 (PubMed:7546292).Curated1
Sequence conflicti277T → A in AAA99541 (PubMed:7546292).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003047380 – 425EECFA…GYQSV → QECSLDDDTILIPIVVGAAL AGLIVIIVIAYIIGRRKSYA GYQTL in isoform LAMP-2C. 1 PublicationAdd BLAST46
Alternative sequenceiVSP_003048381 – 425ECFAD…GYQSV → DCSPEVDYFIVPIAVGAALG GLVVLVIMAYFLGHKKHHNT GYEQF in isoform LAMP-2A. 1 PublicationAdd BLAST45

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10547 mRNA. Translation: AAA99540.1.
U10546 mRNA. Translation: AAA99539.1.
U10548 mRNA. Translation: AAA99541.1.
RefSeqiNP_001001749.1. NM_001001749.1. [Q90617-1]
UniGeneiGga.2942.

Genome annotation databases

EnsembliENSGALT00000013962; ENSGALP00000013946; ENSGALG00000008572. [Q90617-1]
ENSGALT00000067309; ENSGALP00000050708; ENSGALG00000008572. [Q90617-3]
GeneIDi414800.
KEGGigga:414800.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiLAMP2_CHICK
AccessioniPrimary (citable) accession number: Q90617
Secondary accession number(s): Q90616, Q90618
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 5, 2017
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families