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Protein

POU domain, class 3, transcription factor 1

Gene

pou3f1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor that may play important roles in patterning the embryonic brain. Could directly respond to the reception of the sonic hedgehog (shh) signal.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi279 – 33860HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 3, transcription factor 1
Alternative name(s):
POU domain protein 50
Short name:
ZP-50
Gene namesi
Name:pou3f1
Synonyms:pou50, zp50, zp50pou
ORF Names:si:dkey-248g17.1
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437 Componenti: Chromosome 16

Organism-specific databases

ZFINiZDB-GENE-980526-372. pou3f1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 368368POU domain, class 3, transcription factor 1PRO_0000100773Add
BLAST

Expressioni

Tissue specificityi

Predominantly expressed in the embryonic and adult central nervous system.1 Publication

Developmental stagei

Expression starts between 10 and 12 hours post fecondation and is highest between 2 and 3 days after fertilization.1 Publication

Gene expression databases

BgeeiQ90482.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000027757.

Structurei

3D structure databases

ProteinModelPortaliQ90482.
SMRiQ90482. Positions 193-339.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini187 – 26175POU-specificPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG261729.
GeneTreeiENSGT00760000118935.
HOGENOMiHOG000116303.
HOVERGENiHBG053120.
InParanoidiQ90482.
KOiK09365.
OMAiTGHPMSL.
OrthoDBiEOG7DJSMG.
PhylomeDBiQ90482.
TreeFamiTF316413.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR016362. TF_POU_3.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PIRSFiPIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q90482-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATTAQYIPR NNSLPSNPLM HPDSDRMHQG TTYREVQKMM HHEYLQGLAT
60 70 80 90 100
NTGHPMSLTH HQWLPTSNTD WTSGTHIGQA EHNKASVQSR EDLGNGYHRS
110 120 130 140 150
HLVHQPTQNS HHGSWAPTTT HHLSPLSPAS NGHQSLVYSQ TGYTMLSPQP
160 170 180 190 200
SLHHGLRDPL HDDAGSHDNQ MESPQQPFSH HQDHSDEDAP SSDDLEQFAK
210 220 230 240 250
QFKQRRIKLG FTQADVGLAL GTLYGNVFSQ TTICRFEALQ LSFKNMCKLK
260 270 280 290 300
PLLNKWLEET DSNTGSPTNL DKIAAQGRKR KKRTSIEVGV KGALENHFLK
310 320 330 340 350
CPKPSAHEIT TLAGTLQLEK EVVRVWFCNR RQKEKRMTPV GVPHPTMEDV
360
YSQAETPPLH HTLQSPVQ
Length:368
Mass (Da):41,433
Last modified:November 1, 1996 - v1
Checksum:i6C40DCBF4B44386A
GO

Sequence cautioni

The sequence AAI52215.1 differs from that shown.Contaminating sequence. Potential poly-A sequence.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti103 – 1031V → A in AAH66419 (Ref. 3) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96422 mRNA. Translation: CAA65282.1.
BX511161 Genomic DNA. Translation: CAM12975.1.
BC058306 mRNA. Translation: AAH58306.1.
BC066419 mRNA. Translation: AAH66419.1.
BC152214 mRNA. Translation: AAI52215.1. Sequence problems.
RefSeqiNP_571236.1. NM_131161.1.
UniGeneiDr.75088.

Genome annotation databases

EnsembliENSDART00000013148; ENSDARP00000027757; ENSDARG00000009823.
GeneIDi30398.
KEGGidre:30398.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96422 mRNA. Translation: CAA65282.1.
BX511161 Genomic DNA. Translation: CAM12975.1.
BC058306 mRNA. Translation: AAH58306.1.
BC066419 mRNA. Translation: AAH66419.1.
BC152214 mRNA. Translation: AAI52215.1. Sequence problems.
RefSeqiNP_571236.1. NM_131161.1.
UniGeneiDr.75088.

3D structure databases

ProteinModelPortaliQ90482.
SMRiQ90482. Positions 193-339.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000027757.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000013148; ENSDARP00000027757; ENSDARG00000009823.
GeneIDi30398.
KEGGidre:30398.

Organism-specific databases

CTDi5453.
ZFINiZDB-GENE-980526-372. pou3f1.

Phylogenomic databases

eggNOGiNOG261729.
GeneTreeiENSGT00760000118935.
HOGENOMiHOG000116303.
HOVERGENiHBG053120.
InParanoidiQ90482.
KOiK09365.
OMAiTGHPMSL.
OrthoDBiEOG7DJSMG.
PhylomeDBiQ90482.
TreeFamiTF316413.

Miscellaneous databases

NextBioi20806809.
PROiQ90482.

Gene expression databases

BgeeiQ90482.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR016362. TF_POU_3.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PIRSFiPIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complex expression of the zp-50 pou gene in the embryonic zebrafish brain is altered by overexpression of sonic hedgehog."
    Hauptmann G., Gerster T.
    Development 122:1769-1780(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Neurula.
  2. "The zebrafish reference genome sequence and its relationship to the human genome."
    Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.
    , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
    Nature 496:498-503(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tuebingen.
  3. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo and Larval eye.

Entry informationi

Entry nameiPO3F1_DANRE
AccessioniPrimary (citable) accession number: Q90482
Secondary accession number(s): A2BGW5, A7MCK3, Q6NYY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 27, 2015
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.