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Protein

Tiggy-winkle hedgehog protein

Gene

shhb

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Intercellular signal essential for a variety of patterning events during development. Involved in dorso-ventral patterning of the brain and in early patterning of the developing eyes.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi92Calcium 1By similarity1
Metal bindingi93Calcium 1By similarity1
Metal bindingi93Calcium 2By similarity1
Metal bindingi98Calcium 1By similarity1
Metal bindingi128Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi129Calcium 1By similarity1
Metal bindingi129Calcium 2By similarity1
Metal bindingi132Calcium 2By similarity1
Metal bindingi134Calcium 2By similarity1
Metal bindingi143ZincBy similarity1
Metal bindingi150ZincBy similarity1
Metal bindingi185ZincBy similarity1
Sitei270Involved in auto-cleavageBy similarity1
Sitei273Essential for auto-cleavageBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cell-cell signaling Source: InterPro
  • embryonic neurocranium morphogenesis Source: ZFIN
  • embryonic viscerocranium morphogenesis Source: ZFIN
  • endocardial cell differentiation Source: ZFIN
  • forebrain development Source: ZFIN
  • gliogenesis Source: ZFIN
  • neuron differentiation Source: ZFIN
  • neuron fate commitment Source: ZFIN
  • striated muscle cell development Source: ZFIN
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DRE-5358346. Hedgehog ligand biogenesis.
R-DRE-5362798. Release of Hh-Np from the secreting cell.
R-DRE-5632681. Ligand-receptor interactions.
R-DRE-5635838. Activation of SMO.

Protein family/group databases

MEROPSiC46.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Tiggy-winkle hedgehog protein
Short name:
TWHH
Alternative name(s):
Sonic hedgehog protein B
Short name:
SHHB
Cleaved into the following 2 chains:
Gene namesi
Name:shhb
Synonyms:twhh
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 2

Organism-specific databases

ZFINiZDB-GENE-980526-41. shhb.

Subcellular locationi

Tiggy-winkle hedgehog protein N-product :
Tiggy-winkle hedgehog protein C-product :

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000001325627 – 416Tiggy-winkle hedgehog proteinAdd BLAST390
ChainiPRO_000001325727 – 200Tiggy-winkle hedgehog protein N-productAdd BLAST174
ChainiPRO_0000013258201 – 416Tiggy-winkle hedgehog protein C-productAdd BLAST216

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi27N-palmitoyl cysteineBy similarity1
Lipidationi200Cholesterol glycine esterBy similarity1

Post-translational modificationi

The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). This covalent modification appears to play an essential role in restricting the spatial distribution of the protein activity to the cell surface. The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei200 – 201Cleavage; by autolysis2

Keywords - PTMi

Autocatalytic cleavage, Lipoprotein, Palmitate

Proteomic databases

PaxDbiQ90419.

Expressioni

Tissue specificityi

Expressed in the ventral midline of the neural tube and brain. In the developing brain, expression occurs in domains that include a discrete region in the floor of the diencephalon. Not detected in the notochord or developing fin bud.

Gene expression databases

BgeeiENSDARG00000038867.
ExpressionAtlasiQ90419. differential.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000056746.

Structurei

3D structure databases

ProteinModelPortaliQ90419.
SMRiQ90419.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the hedgehog family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3638. Eukaryota.
ENOG410XQA3. LUCA.
GeneTreeiENSGT00390000001117.
HOGENOMiHOG000233428.
HOVERGENiHBG005480.
InParanoidiQ90419.
OMAiILLINTM.
OrthoDBiEOG091G0N90.
PhylomeDBiQ90419.
TreeFamiTF106458.

Family and domain databases

Gene3Di2.170.16.10. 1 hit.
3.30.1380.10. 1 hit.
InterProiIPR001657. Hedgehog.
IPR028992. Hedgehog/Intein_dom.
IPR009045. Hedgehog_sig/DD-Pept_Zn-bd_dom.
IPR000320. Hedgehog_signalling_dom.
IPR001767. Hint_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
[Graphical view]
PfamiPF01085. HH_signal. 1 hit.
PF01079. Hint. 1 hit.
[Graphical view]
PIRSFiPIRSF009400. Peptidase_C46. 1 hit.
PRINTSiPR00632. SONICHHOG.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 1 hit.
SSF55166. SSF55166. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q90419-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVRLHLKQF ALLCFISLLL TPCGLACGPG RGYGKRRHPK KLTPLAYKQF
60 70 80 90 100
IPNVAEKTLG ASGKYEGKIT RNSERFKELI PNYNPDIIFK DEENTNADRL
110 120 130 140 150
MTKRCKDKLN SLAISVMNHW PGVKLRVTEG WDEDGHHLEE SLHYEGRAVD
160 170 180 190 200
ITTSDRDKSK YGMLSRLAVE AGFDWVYYES KAHIHCSVKA ENSVAAKSGG
210 220 230 240 250
CFPGSGTVTL GDGTRKPIKD LKVGDRVLAA DEKGNVLISD FIMFIDHDPT
260 270 280 290 300
TRRQFIVIET SEPFTKLTLT AAHLVFVGNS SAASGITATF ASNVKPGDTV
310 320 330 340 350
LVWEDTCESL KSVTVKRIYT EEHEGSFAPV TAHGTIIVDQ VLASCYAVIE
360 370 380 390 400
NHKWAHWAFA PVRLCHKLMT WLFPARESNV NFQEDGIHWY SNMLFHIGSW
410
LLDRDSFHPL GILHLS
Length:416
Mass (Da):46,576
Last modified:November 1, 1996 - v1
Checksum:i61EC2218309CFE59
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30710 mRNA. Translation: AAC59741.1.
U68237 Genomic DNA. Translation: AAB38678.1.
UniGeneiDr.75752.

Genome annotation databases

EnsembliENSDART00000056747; ENSDARP00000056746; ENSDARG00000038867.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30710 mRNA. Translation: AAC59741.1.
U68237 Genomic DNA. Translation: AAB38678.1.
UniGeneiDr.75752.

3D structure databases

ProteinModelPortaliQ90419.
SMRiQ90419.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000056746.

Protein family/group databases

MEROPSiC46.005.

Proteomic databases

PaxDbiQ90419.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000056747; ENSDARP00000056746; ENSDARG00000038867.

Organism-specific databases

ZFINiZDB-GENE-980526-41. shhb.

Phylogenomic databases

eggNOGiKOG3638. Eukaryota.
ENOG410XQA3. LUCA.
GeneTreeiENSGT00390000001117.
HOGENOMiHOG000233428.
HOVERGENiHBG005480.
InParanoidiQ90419.
OMAiILLINTM.
OrthoDBiEOG091G0N90.
PhylomeDBiQ90419.
TreeFamiTF106458.

Enzyme and pathway databases

ReactomeiR-DRE-5358346. Hedgehog ligand biogenesis.
R-DRE-5362798. Release of Hh-Np from the secreting cell.
R-DRE-5632681. Ligand-receptor interactions.
R-DRE-5635838. Activation of SMO.

Miscellaneous databases

PROiQ90419.

Gene expression databases

BgeeiENSDARG00000038867.
ExpressionAtlasiQ90419. differential.

Family and domain databases

Gene3Di2.170.16.10. 1 hit.
3.30.1380.10. 1 hit.
InterProiIPR001657. Hedgehog.
IPR028992. Hedgehog/Intein_dom.
IPR009045. Hedgehog_sig/DD-Pept_Zn-bd_dom.
IPR000320. Hedgehog_signalling_dom.
IPR001767. Hint_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
[Graphical view]
PfamiPF01085. HH_signal. 1 hit.
PF01079. Hint. 1 hit.
[Graphical view]
PIRSFiPIRSF009400. Peptidase_C46. 1 hit.
PRINTSiPR00632. SONICHHOG.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 1 hit.
SSF55166. SSF55166. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTWHH_DANRE
AccessioniPrimary (citable) accession number: Q90419
Secondary accession number(s): O13212
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.