Tiggy-winkle hedgehog protein precursor

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Q90419 (TWHH_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 92. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Tiggy-winkle hedgehog protein
Short name=TWHH

Alternative name(s):
Sonic hedgehog protein B
Short name=SHHB

Gene names

Name:	shhb
Synonyms:	twhh

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Intercellular signal essential for a variety of patterning events during development. Involved in dorso-ventral patterning of the brain and in early patterning of the developing eyes.
Subcellular location	Tiggy-winkle hedgehog protein N-product: Cell membrane; Lipid-anchor; Extracellular side By similarity. Note: The N-terminal peptide remains associated with the cell surface By similarity. Tiggy-winkle hedgehog protein C-product: Secreted › extracellular space By similarity. Note: The C-terminal peptide diffuses from the cell By similarity.
Tissue specificity	Expressed in the ventral midline of the neural tube and brain. In the developing brain, expression occurs in domains that include a discrete region in the floor of the diencephalon. Not detected in the notochord or developing fin bud.
Post-translational modification	The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). This covalent modification appears to play an essential role in restricting the spatial distribution of the protein activity to the cell surface. The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity By similarity.
Sequence similarities	Belongs to the hedgehog family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane Secreted
Domain	Signal
Molecular function	Developmental protein Hydrolase Protease
PTM	Autocatalytic cleavage Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell-cell signaling Inferred from electronic annotation. Source: InterPro forebrain development Inferred from mutant phenotype. Source: ZFIN gliogenesis Inferred from genetic interaction. Source: ZFIN neuron fate commitment Inferred from genetic interaction. Source: ZFIN proteolysis Inferred from electronic annotation. Source: UniProtKB-KW striated muscle cell development Inferred from genetic interaction. Source: ZFIN
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 26

Potential

Chain

27 – 416

390

Tiggy-winkle hedgehog protein

PRO_0000013256

Chain

27 – 200

174

Tiggy-winkle hedgehog protein N-product

PRO_0000013257

Chain

201 – 416

216

Tiggy-winkle hedgehog protein C-product

PRO_0000013258

Sites

Site

200 – 201

Cleavage; by autolysis

Site

270

Involved in auto-cleavage By similarity

Site

273

Essential for auto-cleavage By similarity

Amino acid modifications

Lipidation

N-palmitoyl cysteine By similarity

Lipidation

200

Cholesterol glycine ester By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q90419 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 61EC2218309CFE59
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FASTA

416

46,576

        10         20         30         40         50         60 
MDVRLHLKQF ALLCFISLLL TPCGLACGPG RGYGKRRHPK KLTPLAYKQF IPNVAEKTLG 

        70         80         90        100        110        120 
ASGKYEGKIT RNSERFKELI PNYNPDIIFK DEENTNADRL MTKRCKDKLN SLAISVMNHW 

       130        140        150        160        170        180 
PGVKLRVTEG WDEDGHHLEE SLHYEGRAVD ITTSDRDKSK YGMLSRLAVE AGFDWVYYES 

       190        200        210        220        230        240 
KAHIHCSVKA ENSVAAKSGG CFPGSGTVTL GDGTRKPIKD LKVGDRVLAA DEKGNVLISD 

       250        260        270        280        290        300 
FIMFIDHDPT TRRQFIVIET SEPFTKLTLT AAHLVFVGNS SAASGITATF ASNVKPGDTV 

       310        320        330        340        350        360 
LVWEDTCESL KSVTVKRIYT EEHEGSFAPV TAHGTIIVDQ VLASCYAVIE NHKWAHWAFA 

       370        380        390        400        410 
PVRLCHKLMT WLFPARESNV NFQEDGIHWY SNMLFHIGSW LLDRDSFHPL GILHLS

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References

[1]	"Patterning activities of vertebrate hedgehog proteins in the developing eye and brain." Ekker S.C., Ungar A.R., Greenstein P., von Kessler D.P., Porter J.A., Moon R.T., Beachy P.A. Curr. Biol. 5:944-955(1995) [PubMed: 7583153] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], AUTOCATALYTIC CLEAVAGE. Tissue: Embryo.
[2]	"Evolutionary analyses of hedgehog and Hoxd-10 genes in fish species closely related to the zebrafish." Zardoya R., Abouheif E., Meyer A. Proc. Natl. Acad. Sci. U.S.A. 93:13036-13041(1996) [PubMed: 8917540] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 110-162. Tissue: Muscle.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U30710 mRNA. Translation: AAC59741.1. U68237 Genomic DNA. Translation: AAB38678.1.
IPI	IPI00489001.
UniGene	Dr.75752.
3D structure databases
ProteinModelPortal	Q90419.
SMR	Q90419. Positions 41-197.
ModBase	Search...
Protein-protein interaction databases
STRING	Q90419.
Protein family/group databases
MEROPS	C46.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000056747; ENSDARP00000056746; ENSDARG00000038867.
Organism-specific databases
ZFIN	ZDB-GENE-980526-41. shhb.
Phylogenomic databases
GeneTree	ENSGT00390000001117.
HOGENOM	HBG403179.
HOVERGEN	HBG005480.
InParanoid	Q90419.
OMA	EYSITAS.
OrthoDB	EOG46WZ8G.
PhylomeDB	Q90419.
Gene expression databases
ArrayExpress	Q90419.
Bgee	Q90419.
Family and domain databases
InterPro	IPR009045. Hedgehog/DD-peptidase. IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR000320. Hedgehog_signaling_dom. IPR001657. Peptidase_C46. IPR001767. Peptidase_C46_hint. [Graphical view]
Gene3D	G3DSA:3.30.1380.10. Hedgehog/DD-pept_Zn-bd. 1 hit.
Pfam	PF01085. HH_signal. 1 hit. PF01079. Hint. 1 hit. [Graphical view]
PIRSF	PIRSF009400. Peptidase_C46. 1 hit.
PRINTS	PR00632. SONICHHOG.
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
SUPFAM	SSF55166. Hedgehog_sig_N. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TWHH_DANRE

Accession

Primary (citable) accession number: Q90419
Secondary accession number(s): O13212

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Cleaved into the following 2 chains:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents