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Protein

Ephrin type-B receptor 2

Gene

EPHB2

Organism
Coturnix japonica (Japanese quail) (Coturnix coturnix japonica)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Functions in axon guidance during development. In addition to axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses (By similarity).By similarity

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei654ATPPROSITE-ProRule annotation1
Active sitei747Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi628 – 636ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Kinase, Receptor, Transferase, Tyrosine-protein kinase
Biological processNeurogenesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.1. 1673.

Names & Taxonomyi

Protein namesi
Recommended name:
Ephrin type-B receptor 2 (EC:2.7.10.1)
Alternative name(s):
EPH-like kinase 5
Short name:
EK5
Short name:
qEK5
Gene namesi
Name:EPHB2
Synonyms:QEK5
OrganismiCoturnix japonica (Japanese quail) (Coturnix coturnix japonica)
Taxonomic identifieri93934 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePerdicinaeCoturnix

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini20 – 544ExtracellularSequence analysisAdd BLAST525
Transmembranei545 – 565HelicalSequence analysisAdd BLAST21
Topological domaini566 – 987CytoplasmicSequence analysisAdd BLAST422

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000001683020 – 987Ephrin type-B receptor 2Add BLAST968

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi63 ↔ 185By similarity
Disulfide bondi98 ↔ 108By similarity
Glycosylationi266N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi337N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi429N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi478N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi483N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ90344.
SMRiQ90344.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 203Eph LBDPROSITE-ProRule annotationAdd BLAST183
Domaini325 – 435Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST111
Domaini436 – 531Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST96
Domaini622 – 885Protein kinasePROSITE-ProRule annotationAdd BLAST264
Domaini914 – 978SAMPROSITE-ProRule annotationAdd BLAST65

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi985 – 987PDZ-bindingSequence analysis3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi185 – 322Cys-richAdd BLAST138

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG062180.

Family and domain databases

CDDicd10477. EphR_LBD_B2. 1 hit.
cd00063. FN3. 2 hits.
Gene3Di2.60.120.260. 1 hit.
2.60.40.10. 2 hits.
InterProiView protein in InterPro
IPR027936. Eph_TM.
IPR034238. EphB2_rcpt_lig-bd.
IPR001090. Ephrin_rcpt_lig-bd_dom.
IPR003961. FN3_dom.
IPR036116. FN3_sf.
IPR008979. Galactose-bd-like_sf.
IPR009030. Growth_fac_rcpt_cys_sf.
IPR013783. Ig-like_fold.
IPR011009. Kinase-like_dom_sf.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001660. SAM.
IPR013761. SAM/pointed_sf.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR011641. Tyr-kin_ephrin_A/B_rcpt-like.
IPR020635. Tyr_kinase_cat_dom.
IPR016257. Tyr_kinase_ephrin_rcpt.
IPR001426. Tyr_kinase_rcpt_V_CS.
PfamiView protein in Pfam
PF14575. EphA2_TM. 1 hit.
PF01404. Ephrin_lbd. 1 hit.
PF07699. Ephrin_rec_like. 1 hit.
PF00041. fn3. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
PF00536. SAM_1. 1 hit.
PIRSFiPIRSF000666. TyrPK_ephrin_receptor. 1 hit.
PRINTSiPR00109. TYRKINASE.
SMARTiView protein in SMART
SM00615. EPH_lbd. 1 hit.
SM01411. Ephrin_rec_like. 1 hit.
SM00060. FN3. 2 hits.
SM00454. SAM. 1 hit.
SM00219. TyrKc. 1 hit.
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF49265. SSF49265. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF56112. SSF56112. 1 hit.
SSF57184. SSF57184. 2 hits.
PROSITEiView protein in PROSITE
PS01186. EGF_2. 1 hit.
PS51550. EPH_LBD. 1 hit.
PS50853. FN3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00790. RECEPTOR_TYR_KIN_V_1. 1 hit.
PS00791. RECEPTOR_TYR_KIN_V_2. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q90344-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPLWFCCLP LALLPLLAAV EETLMDSTTA TAELGWMVHP PSGWEEVSGY
60 70 80 90 100
DENMNTIRTY QVCNVFESSQ NNWLRTKYIR RRGAHRIHVE MKFSVRDCSS
110 120 130 140 150
IPNVPGSCKE TFNLYYYESD FDSATKTFPN WMENPWMKVD TIAADERFSQ
160 170 180 190 200
VDLGGRVMKI NTEVRSFGPV SKNGFYLAFQ DYWGCMSLIA VRVFYRKCPR
210 220 230 240 250
VIQNGADFQE TLSGAESTSL VASRGTCINK AEEVDVPIKQ HCNGDGEWLV
260 270 280 290 300
PIGRCMCRPG YESVANGTVC RGCPSGTFKA SQGDEGCVHC PINSRTTSEG
310 320 330 340 350
ATNCVCRNGY YRADADPVDM PCTTIPSAPQ SVISSVNETS LMLEWTPPRD
360 370 380 390 400
SGGREDLVYN IICKSCGSGR GACTRCGDNV QFAPRQLGLT EPRIYISDLL
410 420 430 440 450
AHTQYTFEIQ AVNGVTDQSP FSPQFASVNI TTNQAAPSAV SIMHQVSRTV
460 470 480 490 500
DSITLSWSQP DQPNGVILDY ELQYYEKNLS ELNSTAVKSP TNTVTVQNLK
510 520 530 540 550
AGTIYVFQVR ARTVAGYGRY SGKMYFQTMT EAEYQTSVQE KLPLIIGSSA
560 570 580 590 600
AGLVFLIAVV VIIIVCNRRG FERADSEYTD KLQHYTSGHM TPGMKIYIDP
610 620 630 640 650
FTYEDPNEAV REFAKEIDIS CVKIEQVIGA GEFGEVCSGH LKLPGKREIF
660 670 680 690 700
VAIKTLKSGY TEKQRRDFLS EASIMGQFDH PNVIHLEGVV TKSSPVMIIT
710 720 730 740 750
EFMENGSLDS FLRQNDGQFT VIQLVGMLRG IAAGMKYLAD MNYVHRDLAA
760 770 780 790 800
RNTLVNSNLV CKVSDFGLSR FLEDDTSDPT YTSALGGKIP IRWTAPEAIQ
810 820 830 840 850
YRKFTSASDV WSYGIVMWEV MSYGERPYWD MTNQDVINAI EQDYRLPPPM
860 870 880 890 900
DCPNALHQLM LDCWQKDRNH RPKFGQIVNT LDKMIRNPNS LKAMAPLSSG
910 920 930 940 950
VNLPLLDRTI PDYTSFNTVD EWLDAIKMSQ YKESYASAGF TTFDIVSQMT
960 970 980
VEDILRVGVT LAGHQKKILN SIQVMRAQMN QIQSVEV
Length:987
Mass (Da):110,331
Last modified:November 1, 1996 - v1
Checksum:i05D6ECC68B718DD7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91737 mRNA. Translation: CAA62862.1.

Similar proteinsi

Entry informationi

Entry nameiEPHB2_COTJA
AccessioniPrimary (citable) accession number: Q90344
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 22, 2017
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families