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Protein

Mitogen-activated protein kinase 14A

Gene

mapk14a

Organism
Cyprinus carpio (Common carp)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Mapk14a is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+1 Publication

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation by the dual specificity kinase, MKK6.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei54ATPPROSITE-ProRule annotation1
Active sitei151Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi31 – 39ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Stress response, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 14A (EC:2.7.11.24)
Short name:
MAP kinase 14A
Short name:
MAPK 14A
Alternative name(s):
Mitogen-activated protein kinase p38a
Short name:
MAP kinase p38a
Short name:
cp38a
Gene namesi
Name:mapk14a
OrganismiCyprinus carpio (Common carp)
Taxonomic identifieri7962 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeCyprinus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001862961 – 361Mitogen-activated protein kinase 14AAdd BLAST361

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei181Phosphothreonine; by MAP2K61 Publication1
Modified residuei183Phosphotyrosine; by MAP2K61 Publication1

Post-translational modificationi

Dually phosphorylated on Thr-181 and Tyr-183, which activates the enzyme.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ90336.

PTM databases

iPTMnetiQ90336.

Expressioni

Tissue specificityi

Exclusively expressed in the ovary.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ90336.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 309Protein kinasePROSITE-ProRule annotationAdd BLAST285

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi181 – 183TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG014652.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01773. P38MAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q90336-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQKERPTFH RQEVNKTIWE VPVRYQNLSP VGSGAYGTVC SAYDEKTGLK
60 70 80 90 100
VAVKKLSRPF QSIIHAKRTY RELRLLKHMK HENVIGLLDV FTPATSLEEF
110 120 130 140 150
NDVYLVTHLM GADLNNIVKC QKLTDDHVQF LIYQILRGLK YIHSADIIHR
160 170 180 190 200
DLKPSNLAVN EDCELKILDF GLARHTDDEM TGYVATRWYR APEIMLNWMH
210 220 230 240 250
YNMTVDIWSV GCIMAELLTG RTLFPGTDHI NQLQQIMRLT GTPPASLISR
260 270 280 290 300
MPSHEARTYI NSLPQMPKRN FSEVFIGANP QAVDLLEKML VLDTDKRITA
310 320 330 340 350
AEALAHPYFA QYHDPDDEPE AEPFDQSFES RELDIEEWKR QTYEEVISFE
360
PPVFDGDEME S
Length:361
Mass (Da):41,660
Last modified:November 1, 1997 - v1
Checksum:iD1B023A21964FBDA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83274 mRNA. Translation: BAA11881.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83274 mRNA. Translation: BAA11881.1.

3D structure databases

ProteinModelPortaliQ90336.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiQ90336.

Proteomic databases

PRIDEiQ90336.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG014652.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01773. P38MAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMK14A_CYPCA
AccessioniPrimary (citable) accession number: Q90336
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 5, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.