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Q90336

- MK14A_CYPCA

UniProt

Q90336 - MK14A_CYPCA

Protein

Mitogen-activated protein kinase 14A

Gene

mapk14a

Organism
Cyprinus carpio (Common carp)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Mapk14a is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.1 Publication

    Enzyme regulationi

    Activated by threonine and tyrosine phosphorylation by the dual specificity kinase, MKK6.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei54 – 541ATPPROSITE-ProRule annotation
    Active sitei151 – 1511Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi31 – 399ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: UniProtKB

    GO - Biological processi

    1. intracellular signal transduction Source: UniProtKB
    2. p38MAPK cascade Source: UniProtKB
    3. positive regulation of myoblast differentiation Source: UniProtKB
    4. positive regulation of myoblast fusion Source: UniProtKB
    5. positive regulation of myotube differentiation Source: UniProtKB
    6. regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    7. response to stress Source: UniProtKB
    8. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Stress response, Transcription, Transcription regulation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase 14A (EC:2.7.11.24)
    Short name:
    MAP kinase 14A
    Short name:
    MAPK 14A
    Alternative name(s):
    Mitogen-activated protein kinase p38a
    Short name:
    MAP kinase p38a
    Short name:
    cp38a
    Gene namesi
    Name:mapk14a
    OrganismiCyprinus carpio (Common carp)
    Taxonomic identifieri7962 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeCyprinus

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 361361Mitogen-activated protein kinase 14APRO_0000186296Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei181 – 1811Phosphothreonine; by MAP2K61 Publication
    Modified residuei183 – 1831Phosphotyrosine; by MAP2K61 Publication

    Post-translational modificationi

    Dually phosphorylated on Thr-181 and Tyr-183, which activates the enzyme.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ90336.

    Expressioni

    Tissue specificityi

    Exclusively expressed in the ovary.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ90336.
    SMRiQ90336. Positions 5-352.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 309285Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi181 – 1833TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    HOVERGENiHBG014652.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR008352. MAPK_p38.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    PRINTSiPR01773. P38MAPKINASE.
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q90336-1 [UniParc]FASTAAdd to Basket

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    MSQKERPTFH RQEVNKTIWE VPVRYQNLSP VGSGAYGTVC SAYDEKTGLK    50
    VAVKKLSRPF QSIIHAKRTY RELRLLKHMK HENVIGLLDV FTPATSLEEF 100
    NDVYLVTHLM GADLNNIVKC QKLTDDHVQF LIYQILRGLK YIHSADIIHR 150
    DLKPSNLAVN EDCELKILDF GLARHTDDEM TGYVATRWYR APEIMLNWMH 200
    YNMTVDIWSV GCIMAELLTG RTLFPGTDHI NQLQQIMRLT GTPPASLISR 250
    MPSHEARTYI NSLPQMPKRN FSEVFIGANP QAVDLLEKML VLDTDKRITA 300
    AEALAHPYFA QYHDPDDEPE AEPFDQSFES RELDIEEWKR QTYEEVISFE 350
    PPVFDGDEME S 361
    Length:361
    Mass (Da):41,660
    Last modified:November 1, 1997 - v1
    Checksum:iD1B023A21964FBDA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D83274 mRNA. Translation: BAA11881.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D83274 mRNA. Translation: BAA11881.1 .

    3D structure databases

    ProteinModelPortali Q90336.
    SMRi Q90336. Positions 5-352.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q90336.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG014652.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR008352. MAPK_p38.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PRINTSi PR01773. P38MAPKINASE.
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a nuclear export signal in MKK6, an activator of the carp p38 mitogen-activated protein kinases."
      Hashimoto H., Fukuda M., Matsuo Y., Yokoyama Y., Nishida E., Toyohara H., Sakaguchi M.
      Eur. J. Biochem. 267:4362-4371(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], COFACTOR, TISSUE SPECIFICITY, PHOSPHORYLATION, ENZYME REGULATION.
      Tissue: Ovary.

    Entry informationi

    Entry nameiMK14A_CYPCA
    AccessioniPrimary (citable) accession number: Q90336
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3