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Q90336 (MK14A_CYPCA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase 14A

Short name=MAP kinase 14A
Short name=MAPK 14A
EC=2.7.11.24
Alternative name(s):
Mitogen-activated protein kinase p38a
Short name=MAP kinase p38a
Short name=cp38a
Gene names
Name:mapk14a
OrganismCyprinus carpio (Common carp)
Taxonomic identifier7962 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeCyprinus

Protein attributes

Sequence length361 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Mapk14a is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium. Ref.1

Enzyme regulation

Activated by threonine and tyrosine phosphorylation by the dual specificity kinase, MKK6. Ref.1

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Tissue specificity

Exclusively expressed in the ovary. Ref.1

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-181 and Tyr-183, which activates the enzyme. Ref.1

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 361361Mitogen-activated protein kinase 14A
PRO_0000186296

Regions

Domain25 – 309285Protein kinase
Nucleotide binding31 – 399ATP By similarity
Motif181 – 1833TXY

Sites

Active site1511Proton acceptor By similarity
Binding site541ATP By similarity

Amino acid modifications

Modified residue1811Phosphothreonine; by MAP2K6
Modified residue1831Phosphotyrosine; by MAP2K6

Sequences

Sequence LengthMass (Da)Tools
Q90336 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: D1B023A21964FBDA

FASTA36141,660
        10         20         30         40         50         60 
MSQKERPTFH RQEVNKTIWE VPVRYQNLSP VGSGAYGTVC SAYDEKTGLK VAVKKLSRPF 

        70         80         90        100        110        120 
QSIIHAKRTY RELRLLKHMK HENVIGLLDV FTPATSLEEF NDVYLVTHLM GADLNNIVKC 

       130        140        150        160        170        180 
QKLTDDHVQF LIYQILRGLK YIHSADIIHR DLKPSNLAVN EDCELKILDF GLARHTDDEM 

       190        200        210        220        230        240 
TGYVATRWYR APEIMLNWMH YNMTVDIWSV GCIMAELLTG RTLFPGTDHI NQLQQIMRLT 

       250        260        270        280        290        300 
GTPPASLISR MPSHEARTYI NSLPQMPKRN FSEVFIGANP QAVDLLEKML VLDTDKRITA 

       310        320        330        340        350        360 
AEALAHPYFA QYHDPDDEPE AEPFDQSFES RELDIEEWKR QTYEEVISFE PPVFDGDEME 


S 

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References

[1]"Identification of a nuclear export signal in MKK6, an activator of the carp p38 mitogen-activated protein kinases."
Hashimoto H., Fukuda M., Matsuo Y., Yokoyama Y., Nishida E., Toyohara H., Sakaguchi M.
Eur. J. Biochem. 267:4362-4371(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], COFACTOR, TISSUE SPECIFICITY, PHOSPHORYLATION, ENZYME REGULATION.
Tissue: Ovary.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D83274 mRNA. Translation: BAA11881.1.

3D structure databases

ProteinModelPortalQ90336.
SMRQ90336. Positions 5-352.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ90336.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG014652.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSPR01773. P38MAPKINASE.
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMK14A_CYPCA
AccessionPrimary (citable) accession number: Q90336
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families