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Q90327 (MK08A_CYPCA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase 8A

Short name=MAP kinase 8A
Short name=MAPK 8A
EC=2.7.11.24
Alternative name(s):
Stress-activated protein kinase JNKa
c-Jun N-terminal kinase A
Gene names
Name:mapk8a
OrganismCyprinus carpio (Common carp)
Taxonomic identifier7962 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeCyprinus

Protein attributes

Sequence length427 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity By similarity. UniProtKB P45983

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium By similarity. UniProtKB P45983

Enzyme regulation

Activated by threonine and tyrosine phosphorylation By similarity. UniProtKB P45983

Tissue specificity

Expressed in brain and ovary. Ref.1

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 427427Mitogen-activated protein kinase 8A
PRO_0000186266

Regions

Domain26 – 321296Protein kinase UniProtKB P53779
Nucleotide binding33 – 386ATP By similarity UniProtKB P53779
Motif183 – 1853TXY

Sites

Active site1511Proton acceptor By similarity UniProtKB P53779
Binding site551ATP By similarity UniProtKB P53779

Amino acid modifications

Modified residue1831Phosphothreonine By similarity UniProtKB P53779
Modified residue1851Phosphotyrosine By similarity UniProtKB P53779

Sequences

Sequence LengthMass (Da)Tools
Q90327 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 518B490DD048120C

FASTA42748,606
        10         20         30         40         50         60 
MNKNKREKEF YSVDVGDSTF TVLKRYQNLR PIGSGAQGIV CSAYDHNLER NVAIKKLSRP 

        70         80         90        100        110        120 
FQNQTHAKRA YRELVLMKYV NHKNIICLLN VFTSQKTLDE FQDVYLVMEL MDANLCQVIQ 

       130        140        150        160        170        180 
MELDHERLSY LLYQMLCGIK HLHSAGIIHR DLKPSNIVVK SDCTLKILDF GLARTAATGL 

       190        200        210        220        230        240 
LMTPYVVTRY YRAPEVILGM GYQANVDIWS VGCILAEMVR HKILFPGRDY IDQWNKVIEQ 

       250        260        270        280        290        300 
LGTPTQEFMM KLNQSVRTYV ENRPRYTGYS FEKLFPDVLF PADSEHNKLK ASQARDLLSK 

       310        320        330        340        350        360 
MLVIDASKRI SVEEALQHPY INVWYDPAEV EAPPPVITDK QLDEREHTVE EWKELIYKEV 

       370        380        390        400        410        420 
LDWEERMKNG AIRGQPSPLG AAVINGSPQP SSSSSINDVS SMSTEPTVAS DTDSSLEASA 


GPLSCCR 

« Hide

References

[1]"Structure and expression of carp mitogen-activated protein kinases homologous to mammalian JNK/SAPK."
Hashimoto H., Matsuo Y., Yokoyama Y., Toyohara H., Sakaguchi M.
J. Biochem. 122:381-386(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Ovary.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D83273 mRNA. Translation: BAA11880.1.
PIRJC5693.

3D structure databases

ProteinModelPortalQ90327.
SMRQ90327. Positions 10-364.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ90327.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG014652.

Enzyme and pathway databases

BRENDA2.7.11.24. 1195.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008351. MAPK_JNK.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSPR01772. JNKMAPKINASE.
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMK08A_CYPCA
AccessionPrimary (citable) accession number: Q90327
Entry history
Integrated into UniProtKB/Swiss-Prot: December 13, 2002
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families