Reviewed, UniProtKB/Swiss-Prot Q90327 (MK08A_CYPCA)

Names and origin

Protein names

Recommended name:
    Mitogen-activated protein kinase 8A
    EC=2.7.11.24
Alternative name(s):
    Stress-activated protein kinase JNKa
    c-Jun N-terminal kinase A

Gene names

Name:

mapk8a

Organism

Cyprinus carpio (Common carp)

Taxonomic identifier

7962 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Cyprinus

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity By similarity. UniProtKB P45983
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity. UniProtKB P45983
Enzyme regulation	Activated by threonine and tyrosine phosphorylation By similarity. UniProtKB P45983
Tissue specificity	Expressed in brain and ovary. Ref.1
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modification	Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	Mitogen-activated protein kinase 8A		PRO_0000186266
Regions
	Domain	26 – 321	296	Protein kinase UniProtKB P53779
	Nucleotide binding	33 – 38	6	ATP By similarity UniProtKB P53779
	Motif	183 – 185	3	TXY
Sites
	Active site	151	1	Proton acceptor By similarity UniProtKB P53779
	Binding site	55	1	ATP By similarity UniProtKB P53779
Amino acid modifications
	Modified residue	183	1	Phosphothreonine By similarity UniProtKB P53779
	Modified residue	185	1	Phosphotyrosine By similarity UniProtKB P53779

Sequences

	Sequence		Length	Mass (Da)
	Q90327-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 518B490DD048120C	FASTA	427	48,606
	10 20 30 40 50 60 MNKNKREKEF YSVDVGDSTF TVLKRYQNLR PIGSGAQGIV CSAYDHNLER NVAIKKLSRP 70 80 90 100 110 120 FQNQTHAKRA YRELVLMKYV NHKNIICLLN VFTSQKTLDE FQDVYLVMEL MDANLCQVIQ 130 140 150 160 170 180 MELDHERLSY LLYQMLCGIK HLHSAGIIHR DLKPSNIVVK SDCTLKILDF GLARTAATGL 190 200 210 220 230 240 LMTPYVVTRY YRAPEVILGM GYQANVDIWS VGCILAEMVR HKILFPGRDY IDQWNKVIEQ 250 260 270 280 290 300 LGTPTQEFMM KLNQSVRTYV ENRPRYTGYS FEKLFPDVLF PADSEHNKLK ASQARDLLSK 310 320 330 340 350 360 MLVIDASKRI SVEEALQHPY INVWYDPAEV EAPPPVITDK QLDEREHTVE EWKELIYKEV 370 380 390 400 410 420 LDWEERMKNG AIRGQPSPLG AAVINGSPQP SSSSSINDVS SMSTEPTVAS DTDSSLEASA GPLSCCR

References

[1]	"Structure and expression of carp mitogen-activated protein kinases homologous to mammalian JNK/SAPK." Hashimoto H., Matsuo Y., Yokoyama Y., Toyohara H., Sakaguchi M. J. Biochem. 122:381-386(1997) [PubMed: 9378717] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Ovary.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D83273 mRNA. Translation: BAA11880.1.
PIR	JC5693.
3D structure databases
HSSP	HSSP built from PDB template 1JNK based on UniProtKB P53779.
SMR	Q90327. Positions 7-362.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q90327.
Enzyme and pathway databases
BRENDA	2.7.11.24. 3298.
Family and domain databases
InterPro	IPR008351. JNK_MAPK. IPR003527. MAP_kinase_CS. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PRINTS	PR01772. JNKMAPKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MK08A_CYPCA

Accession

Primary (citable) accession number: Q90327

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 13, 2002
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)