Reviewed, UniProtKB/Swiss-Prot Q90321 (MP2K2_CYPCA)

Names and origin

Protein names

Recommended name:
    Dual specificity mitogen-activated protein kinase kinase 2
      Short name=MAP kinase kinase 2
      Short name=MAPKK 2
    EC=2.7.12.2
Alternative name(s):
    ERK activator kinase 2
    MAPK/ERK kinase 2
      Short name=MEK2

Gene names

Name:	map2k2
Synonyms:	mkk

Organism

Cyprinus carpio (Common carp)

Taxonomic identifier

7962 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Cyprinus

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Post-translational modification	Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF) regulates positively the kinase activity By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 397	397	Dual specificity mitogen-activated protein kinase kinase 2		PRO_0000086376
Regions
	Domain	69 – 366	298	Protein kinase
	Nucleotide binding	75 – 83	9	ATP By similarity
	Compositional bias	263 – 312	50	Pro-rich
Sites
	Active site	191	1	Proton acceptor By similarity
	Binding site	98	1	ATP By similarity
Amino acid modifications
	Modified residue	219	1	Phosphoserine; by RAF By similarity
	Modified residue	223	1	Phosphoserine; by RAF By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q90321-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C510FF921BF65EDF	FASTA	397	44,147
	10 20 30 40 50 60 MAPKRRPVPL IIAPTGEGQS TNIDAASEAN LEALQRKLGE LDLDEQQRKR LEAFLTQKAQ 70 80 90 100 110 120 VGELKDEDFD PICELGAGNG GVVHKVRHKP SRLVMARKLI HLEIKPAIRN QIIRELQVLH 130 140 150 160 170 180 ECNSPYIVGF YGAFYSDGEI SICMEHMDGG SLDQVLKEAR RIPEEILGKV SIAVLRGLVY 190 200 210 220 230 240 LREKHQIMHR DVKPSNILVN SRGEIKLCDF GVSGQLIDSM ANSFVGTRSY MSPERLQGTH 250 260 270 280 290 300 YSVQSDVWSM GLSLVELAIG RFPIPPPDAK ELEAIFGRPV LDKGGAEGHS MSPRQRPPGR 310 320 330 340 350 360 PVSGHGMDSR PAMAIFELLD YIVNEPPPKL PHGVFTTDFE EFVMKCLMKN PADRADLKML 370 380 390 MGHTFIKRAE VEEVDFAGWM CKTMGLPQPS TPTHSAE

References

[1]	"Molecular cloning and sequencing of a carp cDNA encoding mitogen-activated protein kinase kinase." Huang C.-J., Lee M.-S., Chang G.-D., Huang F.-L., Lo T.-B. Biochim. Biophys. Acta 1220:223-225(1994) [PubMed: 8312367] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L23935 mRNA. Translation: AAA19788.1.
PIR	S41054.
3D structure databases
SMR	Q90321. Positions 57-389.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q90321.
Enzyme and pathway databases
BRENDA	2.7.12.2. 3298.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MP2K2_CYPCA

Accession

Primary (citable) accession number: Q90321

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	January 19, 2010
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)