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Q90270

- PO5F1_DANRE

UniProt

Q90270 - PO5F1_DANRE

Protein

POU domain, class 5, transcription factor 1

Gene

pou5f1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Involved in early development of embryos, especially in the process of gastrulation. May play an important role in establishing and specifying rhombomeric segments. Seems to be required to maintain the cells in a highly undifferentiated state. In contrast to POU2, T-POU2 lacks DNA-binding activity because of its incomplete pou domain structure. Overexpression of POU2 does not have any effect on development, whereas overexpression of t-POU2 causes developmental retardation or arrest before gastrulation.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi343 – 40260HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. protein heterodimerization activity Source: ZFIN
    2. sequence-specific DNA binding Source: ZFIN
    3. sequence-specific DNA binding transcription factor activity Source: ZFIN

    GO - Biological processi

    1. anatomical structure morphogenesis Source: ZFIN
    2. brain development Source: ZFIN
    3. brain segmentation Source: ZFIN
    4. dorsal/ventral pattern formation Source: ZFIN
    5. ectoderm development Source: ZFIN
    6. embryonic pattern specification Source: ZFIN
    7. endoderm formation Source: ZFIN
    8. epiboly involved in gastrulation with mouth forming second Source: ZFIN
    9. fin regeneration Source: ZFIN
    10. hindbrain development Source: ZFIN
    11. mesoderm development Source: ZFIN
    12. morphogenesis of embryonic epithelium Source: ZFIN
    13. positive regulation of transcription, DNA-templated Source: ZFIN
    14. regulation of DNA methylation Source: ZFIN
    15. regulation of endodermal cell fate specification Source: ZFIN
    16. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    POU domain, class 5, transcription factor 1
    Alternative name(s):
    POU domain protein 2
    Gene namesi
    Name:pou5f1
    Synonyms:gp-9, pou-2, pou2
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Chromosome 21

    Organism-specific databases

    ZFINiZDB-GENE-980526-485. pou5f3.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 472472POU domain, class 5, transcription factor 1PRO_0000100752Add
    BLAST

    Expressioni

    Developmental stagei

    Maternally expressed. Present from the one-cell stage to the gastrula stage. Present in all blastomeres until the midblastula stage. The expression is restricted to the epiblast during gastrulation, and to the neural plate after gastrulation. In the adult, expression is limited to the ovary.

    Gene expression databases

    BgeeiQ90270.

    Interactioni

    Protein-protein interaction databases

    STRINGi7955.ENSDARP00000065816.

    Structurei

    3D structure databases

    ProteinModelPortaliQ90270.
    SMRiQ90270. Positions 253-402.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini249 – 32375POU-specificPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi247 – 2504Poly-Glu
    Compositional biasi419 – 4235Poly-Pro

    Sequence similaritiesi

    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
    Contains 1 POU-specific domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    eggNOGiNOG329627.
    GeneTreeiENSGT00750000117482.
    HOGENOMiHOG000063726.
    HOVERGENiHBG053782.
    InParanoidiQ90270.
    KOiK09369.
    OrthoDBiEOG77DJ5X.
    TreeFamiTF316413.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProiIPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR000327. POU_specific.
    [Graphical view]
    PfamiPF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view]
    PRINTSiPR00028. POUDOMAIN.
    SMARTiSM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00035. POU_1. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform POU2 (identifier: Q90270-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTERAQSPTA ADCRPYEVNR AMYPQAAGLD GLGGASLQFA HGMLQDPSLI    50
    FNKAHFNGIT PATAQTFFPF SGDFKTNDLQ GGDFTQPKHW YPFAAPEFTG 100
    QVAGATAATQ PANISPPIGE TREQIKMPSE VKTEKDVEEY GNEENKPPSQ 150
    YHLTAGTSSI PTGVNYYTPW NPNFWPGLSQ ITAQANISQA PPTPSASSPS 200
    LSPSPPGNGF GSPGFFSGGT AQNIPSAQAQ SAPRSSGSSS GGCSNSEEEE 250
    TLTTEDLEQF AKELKHKRIT LGFTQADVGL ALGNLYGKMF SQTTICRFEA 300
    LQLSFKNMCK LKPLLQRWLN EAENSENPQD MYKIERVFVD TRKRKRRTSL 350
    EGTVRSALES YFVKCPKPNT LEITHISDDL GLERDVVRVW FCNRRQKGKR 400
    LALPFDDECV EAQYYEQSPP PPPHMGGTVL PGQGYPGPAH PGGAPALYMP 450
    SLHRPDVFKN GFHPGLVGHL NS 472
    Length:472
    Mass (Da):51,505
    Last modified:November 1, 1996 - v1
    Checksum:iD11678834FBD031F
    GO
    Isoform T-POU2 (identifier: Q90270-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         386-399: VVRVWFCNRRQKGK → CVYGSATVDRRESV
         400-472: Missing.

    Show »
    Length:399
    Mass (Da):43,502
    Checksum:iAB3C4C29A7E5C395
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti160 – 1601I → V in CAA59006. (PubMed:7669688)Curated
    Sequence conflicti245 – 2451N → D in CAA59006. (PubMed:7669688)Curated
    Sequence conflicti462 – 4621F → L in CAA59006. (PubMed:7669688)Curated
    Sequence conflicti471 – 4711N → T in CAA59006. (PubMed:7669688)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei386 – 39914VVRVW…RQKGK → CVYGSATVDRRESV in isoform T-POU2. CuratedVSP_002337Add
    BLAST
    Alternative sequencei400 – 47273Missing in isoform T-POU2. CuratedVSP_002338Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D28548 mRNA. Translation: BAA05901.1.
    X84224 mRNA. Translation: CAA59006.1.
    PIRiA49836.
    B49836.
    RefSeqiNP_571187.1. NM_131112.1.
    UniGeneiDr.258.

    Genome annotation databases

    EnsembliENSDART00000065817; ENSDARP00000065816; ENSDARG00000044774.
    GeneIDi30333.
    KEGGidre:30333.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D28548 mRNA. Translation: BAA05901.1 .
    X84224 mRNA. Translation: CAA59006.1 .
    PIRi A49836.
    B49836.
    RefSeqi NP_571187.1. NM_131112.1.
    UniGenei Dr.258.

    3D structure databases

    ProteinModelPortali Q90270.
    SMRi Q90270. Positions 253-402.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7955.ENSDARP00000065816.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSDART00000065817 ; ENSDARP00000065816 ; ENSDARG00000044774 .
    GeneIDi 30333.
    KEGGi dre:30333.

    Organism-specific databases

    CTDi 30333.
    ZFINi ZDB-GENE-980526-485. pou5f3.

    Phylogenomic databases

    eggNOGi NOG329627.
    GeneTreei ENSGT00750000117482.
    HOGENOMi HOG000063726.
    HOVERGENi HBG053782.
    InParanoidi Q90270.
    KOi K09369.
    OrthoDBi EOG77DJ5X.
    TreeFami TF316413.

    Miscellaneous databases

    NextBioi 20806765.

    Gene expression databases

    Bgeei Q90270.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProi IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR000327. POU_specific.
    [Graphical view ]
    Pfami PF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view ]
    PRINTSi PR00028. POUDOMAIN.
    SMARTi SM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00035. POU_1. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel POU domain gene, zebrafish pou2: expression and roles of two alternatively spliced twin products in early development."
      Takeda H., Matsuzaki T., Oki T., Miyagawa T., Amanuma H.
      Genes Dev. 8:45-59(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
      Strain: AB.
    2. "Pou-2 -- a zebrafish gene active during cleavage stages and in the early hindbrain."
      Hauptmann G., Gerster T.
      Mech. Dev. 51:127-138(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Neurula.

    Entry informationi

    Entry nameiPO5F1_DANRE
    AccessioniPrimary (citable) accession number: Q90270
    Secondary accession number(s): Q90483
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3