Q90270 (PO5F1_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
POU domain, class 5, transcription factor 1

Alternative name(s):
POU domain protein 2

Gene names

Name:	pou5f1
Synonyms:	gp-9, pou-2, pou2

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Cypriniphysi › Cypriniformes › Cyprinoidea › Cyprinidae › Danio

Protein attributes

Sequence length	472 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in early development of embryos, especially in the process of gastrulation. May play an important role in establishing and specifying rhombomeric segments. Seems to be required to maintain the cells in a highly undifferentiated state. In contrast to POU2, T-POU2 lacks DNA-binding activity because of its incomplete pou domain structure. Overexpression of POU2 does not have any effect on development, whereas overexpression of t-POU2 causes developmental retardation or arrest before gastrulation.
Subcellular location	Nucleus.
Developmental stage	Maternally expressed. Present from the one-cell stage to the gastrula stage. Present in all blastomeres until the midblastula stage. The expression is restricted to the epiblast during gastrulation, and to the neural plate after gastrulation. In the adult, expression is limited to the ovary.
Sequence similarities	Belongs to the POU transcription factor family. Class-7 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	brain segmentation Inferred from mutant phenotype PubMed 11923201. Source: ZFIN dorsal/ventral pattern formation Inferred from mutant phenotype PubMed 16775002. Source: ZFIN ectoderm development Inferred from mutant phenotype PubMed 11861475. Source: ZFIN embryonic pattern specification Inferred from mutant phenotype PubMed 11861475PubMed 15366005. Source: ZFIN endoderm formation Inferred from mutant phenotype PubMed 14711414PubMed 14723850. Source: ZFIN epiboly involved in gastrulation with mouth forming second Inferred from mutant phenotype PubMed 18215655. Source: ZFIN fin regeneration Inferred from mutant phenotype PubMed 20089153. Source: ZFIN hindbrain development Inferred from mutant phenotype PubMed 11923201. Source: ZFIN mesoderm development Inferred from mutant phenotype PubMed 11861475. Source: ZFIN morphogenesis of embryonic epithelium Inferred from mutant phenotype PubMed 11861475. Source: ZFIN positive regulation of transcription, DNA-dependent Inferred from genetic interaction PubMed 14723850. Source: ZFIN regulation of DNA methylation Inferred from genetic interaction PubMed 20850014. Source: ZFIN transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from genetic interaction PubMed 14723850. Source: ZFIN
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform POU2 (identifier: Q90270-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform T-POU2 (identifier: Q90270-2)

The sequence of this isoform differs from the canonical sequence as follows:
     386-399: VVRVWFCNRRQKGK → CVYGSATVDRRESV
     400-472: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 472	472	POU domain, class 5, transcription factor 1		PRO_0000100752
Regions
	Domain	249 – 323	75	POU-specific
	DNA binding	343 – 402	60	Homeobox
	Compositional bias	247 – 250	4	Poly-Glu
	Compositional bias	419 – 423	5	Poly-Pro
Natural variations
	Alternative sequence	386 – 399	14	VVRVW…RQKGK → CVYGSATVDRRESV in isoform T-POU2.		VSP_002337
	Alternative sequence	400 – 472	73	Missing in isoform T-POU2.		VSP_002338
Experimental info
	Sequence conflict	160	1	I → V in CAA59006. Ref.2
	Sequence conflict	245	1	N → D in CAA59006. Ref.2
	Sequence conflict	462	1	F → L in CAA59006. Ref.2
	Sequence conflict	471	1	N → T in CAA59006. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform POU2 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D11678834FBD031F	FASTA	472	51,505
	10 20 30 40 50 60 MTERAQSPTA ADCRPYEVNR AMYPQAAGLD GLGGASLQFA HGMLQDPSLI FNKAHFNGIT 70 80 90 100 110 120 PATAQTFFPF SGDFKTNDLQ GGDFTQPKHW YPFAAPEFTG QVAGATAATQ PANISPPIGE 130 140 150 160 170 180 TREQIKMPSE VKTEKDVEEY GNEENKPPSQ YHLTAGTSSI PTGVNYYTPW NPNFWPGLSQ 190 200 210 220 230 240 ITAQANISQA PPTPSASSPS LSPSPPGNGF GSPGFFSGGT AQNIPSAQAQ SAPRSSGSSS 250 260 270 280 290 300 GGCSNSEEEE TLTTEDLEQF AKELKHKRIT LGFTQADVGL ALGNLYGKMF SQTTICRFEA 310 320 330 340 350 360 LQLSFKNMCK LKPLLQRWLN EAENSENPQD MYKIERVFVD TRKRKRRTSL EGTVRSALES 370 380 390 400 410 420 YFVKCPKPNT LEITHISDDL GLERDVVRVW FCNRRQKGKR LALPFDDECV EAQYYEQSPP 430 440 450 460 470 PPPHMGGTVL PGQGYPGPAH PGGAPALYMP SLHRPDVFKN GFHPGLVGHL NS
	Isoform T-POU2 [UniParc]. Checksum: AB3C4C29A7E5C395	FASTA	399	43,502
	10 20 30 40 50 60 MTERAQSPTA ADCRPYEVNR AMYPQAAGLD GLGGASLQFA HGMLQDPSLI FNKAHFNGIT 70 80 90 100 110 120 PATAQTFFPF SGDFKTNDLQ GGDFTQPKHW YPFAAPEFTG QVAGATAATQ PANISPPIGE 130 140 150 160 170 180 TREQIKMPSE VKTEKDVEEY GNEENKPPSQ YHLTAGTSSI PTGVNYYTPW NPNFWPGLSQ 190 200 210 220 230 240 ITAQANISQA PPTPSASSPS LSPSPPGNGF GSPGFFSGGT AQNIPSAQAQ SAPRSSGSSS 250 260 270 280 290 300 GGCSNSEEEE TLTTEDLEQF AKELKHKRIT LGFTQADVGL ALGNLYGKMF SQTTICRFEA 310 320 330 340 350 360 LQLSFKNMCK LKPLLQRWLN EAENSENPQD MYKIERVFVD TRKRKRRTSL EGTVRSALES 370 380 390 YFVKCPKPNT LEITHISDDL GLERDCVYGS ATVDRRESV

References

[1]	"A novel POU domain gene, zebrafish pou2: expression and roles of two alternatively spliced twin products in early development." Takeda H., Matsuzaki T., Oki T., Miyagawa T., Amanuma H. Genes Dev. 8:45-59(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING. Strain: AB.
[2]	"Pou-2 -- a zebrafish gene active during cleavage stages and in the early hindbrain." Hauptmann G., Gerster T. Mech. Dev. 51:127-138(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Neurula.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D28548 mRNA. Translation: BAA05901.1. X84224 mRNA. Translation: CAA59006.1.
IPI	IPI00508081. IPI00552007.
PIR	A49836. B49836.
RefSeq	NP_571187.1. NM_131112.1.
UniGene	Dr.258.
3D structure databases
ProteinModelPortal	Q90270.
SMR	Q90270. Positions 253-402.
ModBase	Search...
Protein-protein interaction databases
STRING	7955.ENSDARP00000065816.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000065817; ENSDARP00000065816; ENSDARG00000044774.
GeneID	30333.
KEGG	dre:30333.
Organism-specific databases
CTD	5460.
ZFIN	ZDB-GENE-980526-485. pou5f1.
Phylogenomic databases
eggNOG	NOG329627.
GeneTree	ENSGT00700000104015.
HOGENOM	HOG000063726.
HOVERGEN	HBG053782.
InParanoid	Q90270.
KO	K09369.
OrthoDB	EOG46MBK1.
Gene expression databases
Bgee	Q90270.
Family and domain databases
Gene3D	1.10.10.60. 1 hit. 1.10.260.40. 1 hit.
InterPro	IPR017970. Homeobox_CS. IPR001356. Homeodomain. IPR009057. Homeodomain-like. IPR010982. Lambda_DNA-bd_dom. IPR013847. POU. IPR000327. POU_specific. [Graphical view]
Pfam	PF00046. Homeobox. 1 hit. PF00157. Pou. 1 hit. [Graphical view]
PRINTS	PR00028. POUDOMAIN.
SMART	SM00389. HOX. 1 hit. SM00352. POU. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit. SSF47413. Lambda_like_DNA. 1 hit.
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. PS00035. POU_1. 1 hit. PS00465. POU_2. 1 hit. PS51179. POU_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20806765.

Entry information

Entry name

PO5F1_DANRE

Accession

Primary (citable) accession number: Q90270
Secondary accession number(s): Q90483

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program