Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q90270 (PO5F1_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
POU domain, class 5, transcription factor 1
Alternative name(s):
POU domain protein 2
Gene names
Name:pou5f1
Synonyms:gp-9, pou-2, pou2
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length472 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in early development of embryos, especially in the process of gastrulation. May play an important role in establishing and specifying rhombomeric segments. Seems to be required to maintain the cells in a highly undifferentiated state. In contrast to POU2, T-POU2 lacks DNA-binding activity because of its incomplete pou domain structure. Overexpression of POU2 does not have any effect on development, whereas overexpression of t-POU2 causes developmental retardation or arrest before gastrulation.

Subcellular location

Nucleus.

Developmental stage

Maternally expressed. Present from the one-cell stage to the gastrula stage. Present in all blastomeres until the midblastula stage. The expression is restricted to the epiblast during gastrulation, and to the neural plate after gastrulation. In the adult, expression is limited to the ovary.

Sequence similarities

Belongs to the POU transcription factor family. Class-7 subfamily.

Contains 1 homeobox DNA-binding domain.

Contains 1 POU-specific domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainHomeobox
   LigandDNA-binding
   Molecular functionDevelopmental protein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processbrain segmentation

Inferred from mutant phenotype PubMed 11923201. Source: ZFIN

dorsal/ventral pattern formation

Inferred from mutant phenotype PubMed 16775002. Source: ZFIN

ectoderm development

Inferred from mutant phenotype PubMed 11861475. Source: ZFIN

embryonic pattern specification

Inferred from mutant phenotype PubMed 11861475PubMed 15366005. Source: ZFIN

endoderm formation

Inferred from mutant phenotype PubMed 14711414PubMed 14723850. Source: ZFIN

epiboly involved in gastrulation with mouth forming second

Inferred from mutant phenotype PubMed 18215655. Source: ZFIN

fin regeneration

Inferred from mutant phenotype PubMed 20089153. Source: ZFIN

hindbrain development

Inferred from mutant phenotype PubMed 11923201. Source: ZFIN

mesoderm development

Inferred from mutant phenotype PubMed 11861475. Source: ZFIN

morphogenesis of embryonic epithelium

Inferred from mutant phenotype PubMed 11861475. Source: ZFIN

positive regulation of transcription, DNA-templated

Inferred from genetic interaction PubMed 14723850. Source: ZFIN

regulation of DNA methylation

Inferred from genetic interaction PubMed 20850014. Source: ZFIN

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionsequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding transcription factor activity

Inferred from genetic interaction PubMed 14723850. Source: ZFIN

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform POU2 (identifier: Q90270-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform T-POU2 (identifier: Q90270-2)

The sequence of this isoform differs from the canonical sequence as follows:
     386-399: VVRVWFCNRRQKGK → CVYGSATVDRRESV
     400-472: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 472472POU domain, class 5, transcription factor 1
PRO_0000100752

Regions

Domain249 – 32375POU-specific
DNA binding343 – 40260Homeobox
Compositional bias247 – 2504Poly-Glu
Compositional bias419 – 4235Poly-Pro

Natural variations

Alternative sequence386 – 39914VVRVW…RQKGK → CVYGSATVDRRESV in isoform T-POU2.
VSP_002337
Alternative sequence400 – 47273Missing in isoform T-POU2.
VSP_002338

Experimental info

Sequence conflict1601I → V in CAA59006. Ref.2
Sequence conflict2451N → D in CAA59006. Ref.2
Sequence conflict4621F → L in CAA59006. Ref.2
Sequence conflict4711N → T in CAA59006. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform POU2 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: D11678834FBD031F

FASTA47251,505
        10         20         30         40         50         60 
MTERAQSPTA ADCRPYEVNR AMYPQAAGLD GLGGASLQFA HGMLQDPSLI FNKAHFNGIT 

        70         80         90        100        110        120 
PATAQTFFPF SGDFKTNDLQ GGDFTQPKHW YPFAAPEFTG QVAGATAATQ PANISPPIGE 

       130        140        150        160        170        180 
TREQIKMPSE VKTEKDVEEY GNEENKPPSQ YHLTAGTSSI PTGVNYYTPW NPNFWPGLSQ 

       190        200        210        220        230        240 
ITAQANISQA PPTPSASSPS LSPSPPGNGF GSPGFFSGGT AQNIPSAQAQ SAPRSSGSSS 

       250        260        270        280        290        300 
GGCSNSEEEE TLTTEDLEQF AKELKHKRIT LGFTQADVGL ALGNLYGKMF SQTTICRFEA 

       310        320        330        340        350        360 
LQLSFKNMCK LKPLLQRWLN EAENSENPQD MYKIERVFVD TRKRKRRTSL EGTVRSALES 

       370        380        390        400        410        420 
YFVKCPKPNT LEITHISDDL GLERDVVRVW FCNRRQKGKR LALPFDDECV EAQYYEQSPP 

       430        440        450        460        470 
PPPHMGGTVL PGQGYPGPAH PGGAPALYMP SLHRPDVFKN GFHPGLVGHL NS 

« Hide

Isoform T-POU2 [UniParc].

Checksum: AB3C4C29A7E5C395
Show »

FASTA39943,502

References

[1]"A novel POU domain gene, zebrafish pou2: expression and roles of two alternatively spliced twin products in early development."
Takeda H., Matsuzaki T., Oki T., Miyagawa T., Amanuma H.
Genes Dev. 8:45-59(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
Strain: AB.
[2]"Pou-2 -- a zebrafish gene active during cleavage stages and in the early hindbrain."
Hauptmann G., Gerster T.
Mech. Dev. 51:127-138(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Neurula.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D28548 mRNA. Translation: BAA05901.1.
X84224 mRNA. Translation: CAA59006.1.
PIRA49836.
B49836.
RefSeqNP_571187.1. NM_131112.1.
UniGeneDr.258.

3D structure databases

ProteinModelPortalQ90270.
SMRQ90270. Positions 253-402.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7955.ENSDARP00000065816.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSDART00000065817; ENSDARP00000065816; ENSDARG00000044774.
GeneID30333.
KEGGdre:30333.

Organism-specific databases

CTD30333.
ZFINZDB-GENE-980526-485. pou5f3.

Phylogenomic databases

eggNOGNOG329627.
GeneTreeENSGT00730000110351.
HOGENOMHOG000063726.
HOVERGENHBG053782.
InParanoidQ90270.
KOK09369.
OrthoDBEOG77DJ5X.
TreeFamTF316413.

Gene expression databases

BgeeQ90270.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSPR00028. POUDOMAIN.
SMARTSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20806765.

Entry information

Entry namePO5F1_DANRE
AccessionPrimary (citable) accession number: Q90270
Secondary accession number(s): Q90483
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families