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Protein

Zinc metalloproteinase/disintegrin

Gene
N/A
Organism
Gloydius halys (Chinese water mocassin) (Agkistrodon halys)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Snake venom metalloproteinase: impairs hemostasis in the envenomed animal.By similarity
Disintegrin halystatin: inhibits platelet aggregation and bone resorption.1 Publication

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi333 – 3331Zinc; catalyticBy similarity
Active sitei334 – 3341PROSITE-ProRule annotation
Metal bindingi337 – 3371Zinc; catalyticBy similarity
Metal bindingi343 – 3431Zinc; catalyticBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Cell adhesion impairing toxin, Hemostasis impairing toxin, Hydrolase, Metalloprotease, Platelet aggregation inhibiting toxin, Protease, Toxin

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM12.134.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc metalloproteinase/disintegrin
Cleaved into the following 2 chains:
Alternative name(s):
Disintegrin adinbitor
Disintegrin brevicaudin-1b
OrganismiGloydius halys (Chinese water mocassin) (Agkistrodon halys)
Taxonomic identifieri8714 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeGloydius

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Propeptidei21 – 190170By similarityPRO_0000424450Add
BLAST
Chaini191 – 407217Snake venom metalloproteinasePRO_5000139751Add
BLAST
Chaini408 – 48073Disintegrin halystatinPRO_5000139752Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi259 – 2591N-linked (GlcNAc...)Sequence analysis
Glycosylationi279 – 2791N-linked (GlcNAc...)Sequence analysis
Disulfide bondi308 ↔ 386By similarity
Disulfide bondi348 ↔ 370By similarity
Disulfide bondi350 ↔ 353By similarity
Disulfide bondi413 ↔ 428By similarity
Disulfide bondi415 ↔ 423By similarity
Disulfide bondi422 ↔ 445By similarity
Disulfide bondi436 ↔ 442By similarity
Disulfide bondi441 ↔ 466By similarity
Disulfide bondi454 ↔ 473By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ90220.
SMRiQ90220. Positions 192-391, 408-480.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini197 – 391195Peptidase M12BPROSITE-ProRule annotationAdd
BLAST
Domaini399 – 48082DisintegrinPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi458 – 4603Cell attachment site

Sequence similaritiesi

Contains 1 disintegrin domain.PROSITE-ProRule annotation
Contains 1 peptidase M12B domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG006978.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR018358. Disintegrin_CS.
IPR001762. Disintegrin_dom.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q90220-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIQVLLITIC LAVFPFQGSS IVLDSGNLNE FEVVYPEKVT ALPRAAVKNK
60 70 80 90 100
YEDAMQYEFK VNGEPLLLHL ERNKGLFSDD YSEIHYSPDA REISAYPSVE
110 120 130 140 150
DHCFYHGRVE NDADSTASLS ACDGLKAHFK IQGEMYLIEP LEVSDTDAHA
160 170 180 190 200
VFKYENVEKE DEPPKMCGVT QNWESYESTK KASQLNVSPD QQRFPQRFIK
210 220 230 240 250
LAIYVDHGMY TKYAGNSERI TKRVHQMINN INMMCRALNI VTSLSVLRIW
260 270 280 290 300
SEKDLITVNA SAPSSLTLFG AWRETVLLNR TSHDHAQLMT ATIFNGNVIG
310 320 330 340 350
RAPVGGMCDP KRSVAIVRDH NAILFIVAVT MTHEMGHNLG MHHDEDKCNC
360 370 380 390 400
NTCIMSKVLS RQPSYEFSDC NENEYQTYVT DHSPQCILND PLRPDTVSTP
410 420 430 440 450
VSGNELLEAG EECDCGSPGN PCCDAATCKL RQGAQCAEGL CCDQCRFMKK
460 470 480
GTVCRIARGD DMDDYCNGIS AGCPRNPFHA
Length:480
Mass (Da):53,620
Last modified:November 1, 1996 - v1
Checksum:i5CF5E6476511B3D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D28870 mRNA. Translation: BAA06025.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D28870 mRNA. Translation: BAA06025.1.

3D structure databases

ProteinModelPortaliQ90220.
SMRiQ90220. Positions 192-391, 408-480.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiM12.134.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG006978.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR018358. Disintegrin_CS.
IPR001762. Disintegrin_dom.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Halystatin, a novel disintegrin from agkistrodon halys, is a potent inhibitor of bone resorption and platelet aggregation."
    Fujisawa Y., Kuroda S., Notoya K., Konishi H., Terashita Z.
    Takeda Kenkyusho Ho 53:39-56(1994)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Tissue: Venom gland.

Entry informationi

Entry nameiVM2H1_GLOHA
AccessioniPrimary (citable) accession number: Q90220
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2013
Last sequence update: November 1, 1996
Last modified: October 14, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Miscellaneous

The disintegrin belongs to the medium disintegrin subfamily.

Caution

The disintegrin is also encoded by another precursor (AC Q1PBD1).Curated

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.