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Protein

KP4 killer toxin

Gene

M2A

Organism
Ustilago maydis P4 virus (UmV4) (UmV-P4)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This protein is lethal to sensitive cells of the same or related species. It specifically inhibits voltage-gated calcium channels. It inhibits cell growth and division by blocking calcium import.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
KP4 killer toxin
Alternative name(s):
Fungal toxin KP4
Killer protein 4
Gene namesi
Name:M2A
OrganismiUstilago maydis P4 virus (UmV4) (UmV-P4)
Taxonomic identifieri11009 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesTotiviridaeTotivirus
Virus hostiUstilago maydis (Corn smut fungus) [TaxID: 5270]

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Biotechnological usei

The KP4 toxin expressed in transgenic plant can render them resistant to KP4-susceptible fungal pathogens such as grass smut fungi. Successful tests have been made in tobacco and wheat.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 222 PublicationsAdd BLAST22
ChainiPRO_000004133823 – 127KP4 killer toxinAdd BLAST105

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi27 ↔ 100
Disulfide bondi33 ↔ 103
Disulfide bondi49 ↔ 89
Disulfide bondi57 ↔ 82
Disulfide bondi66 ↔ 127

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1127
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi31 – 35Combined sources5
Helixi40 – 50Combined sources11
Beta strandi54 – 56Combined sources3
Beta strandi63 – 66Combined sources4
Beta strandi71 – 80Combined sources10
Helixi85 – 98Combined sources14
Beta strandi104 – 109Combined sources6
Helixi114 – 116Combined sources3
Beta strandi118 – 125Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KPTX-ray1.75A/B23-127[»]
ProteinModelPortaliQ90121.
SMRiQ90121.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ90121.

Family & Domainsi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.30.430.10. 1 hit.
InterProiIPR015131. Killer_tox_Kp4.
IPR011329. Killer_tox_Kp4/SMK.
[Graphical view]
PfamiPF09044. Kp4. 1 hit.
[Graphical view]
SUPFAMiSSF55221. SSF55221. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q90121-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQIINVVYSF LFAAAMLPVV HSLGINCRGS SQCGLSGGNL MVRIRDQACG
60 70 80 90 100
NQGQTWCPGE RRAKVCGTGN SISAYVQSTN NCISGTEACR HLTNLVNHGC
110 120
RVCGSDPLYA GNDVSRGQLT VNYVNSC
Length:127
Mass (Da):13,489
Last modified:November 1, 1996 - v1
Checksum:i34CAA729C9A63E1E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti56W → I AA sequence (PubMed:8145639).Curated1
Sequence conflicti66C → E AA sequence (PubMed:8145639).Curated1
Sequence conflicti67G → C AA sequence (PubMed:8145639).Curated1
Sequence conflicti73S → D AA sequence (PubMed:8145639).Curated1
Sequence conflicti74A → H AA sequence (PubMed:8145639).Curated1
Sequence conflicti79T → S AA sequence (PubMed:8145639).Curated1

Mass spectrometryi

Molecular mass is 11045±11 Da from positions 23 - 127. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12226 Genomic RNA. Translation: AAA89185.1.
U25179 Genomic RNA. Translation: AAA75041.1.
PIRiS40034.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12226 Genomic RNA. Translation: AAA89185.1.
U25179 Genomic RNA. Translation: AAA75041.1.
PIRiS40034.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KPTX-ray1.75A/B23-127[»]
ProteinModelPortaliQ90121.
SMRiQ90121.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiQ90121.

Family and domain databases

Gene3Di3.30.430.10. 1 hit.
InterProiIPR015131. Killer_tox_Kp4.
IPR011329. Killer_tox_Kp4/SMK.
[Graphical view]
PfamiPF09044. Kp4. 1 hit.
[Graphical view]
SUPFAMiSSF55221. SSF55221. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKP4T_UMV4
AccessioniPrimary (citable) accession number: Q90121
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be glycosylated, but this does not seem to be the case according to PubMed:8145639.1 Publication

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.