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Q90121

- KP4T_UMV4

UniProt

Q90121 - KP4T_UMV4

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Protein

KP4 killer toxin

Gene
M2A
Organism
Ustilago maydis P4 virus (UmV4) (UmV-P4)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

This protein is lethal to sensitive cells of the same or related species. It specifically inhibits voltage-gated calcium channels. It inhibits cell growth and division by blocking calcium import.1 Publication

GO - Biological processi

  1. pathogenesis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
KP4 killer toxin
Alternative name(s):
Fungal toxin KP4
Killer protein 4
Gene namesi
Name:M2A
OrganismiUstilago maydis P4 virus (UmV4) (UmV-P4)
Taxonomic identifieri11009 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesTotiviridaeTotivirus
Virus hostiUstilago maydis (Corn smut fungus) [TaxID: 5270]

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Biotechnological usei

The KP4 toxin expressed in transgenic plant can render them resistant to KP4-susceptible fungal pathogens such as grass smut fungi. Successful tests have been made in tobacco and wheat.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 22222 PublicationsAdd
BLAST
Chaini23 – 127105KP4 killer toxinPRO_0000041338Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi27 ↔ 100
Disulfide bondi33 ↔ 103
Disulfide bondi49 ↔ 89
Disulfide bondi57 ↔ 82
Disulfide bondi66 ↔ 127

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1
127
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi31 – 355
Helixi40 – 5011
Beta strandi54 – 563
Beta strandi63 – 664
Beta strandi71 – 8010
Helixi85 – 9814
Beta strandi104 – 1096
Helixi114 – 1163
Beta strandi118 – 1258

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KPTX-ray1.75A/B23-127[»]
ProteinModelPortaliQ90121.
SMRiQ90121. Positions 23-127.

Miscellaneous databases

EvolutionaryTraceiQ90121.

Family & Domainsi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.30.430.10. 1 hit.
InterProiIPR015131. Killer_tox_Kp4.
IPR011329. Killer_tox_Kp4/SMK.
[Graphical view]
PfamiPF09044. Kp4. 1 hit.
[Graphical view]
SUPFAMiSSF55221. SSF55221. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q90121-1 [UniParc]FASTAAdd to Basket

« Hide

MQIINVVYSF LFAAAMLPVV HSLGINCRGS SQCGLSGGNL MVRIRDQACG    50
NQGQTWCPGE RRAKVCGTGN SISAYVQSTN NCISGTEACR HLTNLVNHGC 100
RVCGSDPLYA GNDVSRGQLT VNYVNSC 127
Length:127
Mass (Da):13,489
Last modified:November 1, 1996 - v1
Checksum:i34CAA729C9A63E1E
GO

Mass spectrometryi

Molecular mass is 11045±11 Da from positions 23 - 127. Determined by MALDI. 1 Publication

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti56 – 561W → I AA sequence 1 Publication
Sequence conflicti66 – 661C → E AA sequence 1 Publication
Sequence conflicti67 – 671G → C AA sequence 1 Publication
Sequence conflicti73 – 731S → D AA sequence 1 Publication
Sequence conflicti74 – 741A → H AA sequence 1 Publication
Sequence conflicti79 – 791T → S AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L12226 Genomic RNA. Translation: AAA89185.1.
U25179 Genomic RNA. Translation: AAA75041.1.
PIRiS40034.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L12226 Genomic RNA. Translation: AAA89185.1 .
U25179 Genomic RNA. Translation: AAA75041.1 .
PIRi S40034.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1KPT X-ray 1.75 A/B 23-127 [» ]
ProteinModelPortali Q90121.
SMRi Q90121. Positions 23-127.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei Q90121.

Family and domain databases

Gene3Di 3.30.430.10. 1 hit.
InterProi IPR015131. Killer_tox_Kp4.
IPR011329. Killer_tox_Kp4/SMK.
[Graphical view ]
Pfami PF09044. Kp4. 1 hit.
[Graphical view ]
SUPFAMi SSF55221. SSF55221. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Structure and heterologous expression of the Ustilago maydis viral toxin KP4."
    Park C.-M., Bruenn J.A., Ganesa C., Flurkey W.F., Bozarth R.F., Koltin Y.
    Mol. Microbiol. 11:155-164(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], PROTEIN SEQUENCE OF 23-79 AND 124-127, MASS SPECTROMETRY.
    Strain: 77.
  2. Gu F., Khimani A.K., Flurkey W.F., Bozarth R.F., Smith T.J., Rane S.
    Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: M2B.
  3. "Ustilago maydis virus P4 killer toxin: characterization, partial amino terminus sequence, and evidence for glycosylation."
    Ganesa C., Flurkey W.H., Randhawa Z.I., Bozarth R.F.
    Arch. Biochem. Biophys. 286:195-200(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, PROTEIN SEQUENCE OF 23-52.
  4. "KP4 fungal toxin inhibits growth in Ustilago maydis by blocking calcium uptake."
    Gage M.J., Bruenn J., Fischer M., Sanders D., Smith T.J.
    Mol. Microbiol. 41:775-785(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Structure and function of a virally encoded fungal toxin from Ustilago maydis: a fungal and mammalian Ca2+ channel inhibitor."
    Gu F., Khimani A., Rane S.G., Flurkey W.H., Bozarth R.F., Smith T.J.
    Structure 3:805-814(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).

Entry informationi

Entry nameiKP4T_UMV4
AccessioniPrimary (citable) accession number: Q90121
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: October 16, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Caution

Was originally (1 Publication) thought to be glycosylated, but this does not seem to be the case according to 1 Publication.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3