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Q90121 (KP4T_UMV4) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
KP4 killer toxin
Alternative name(s):
Fungal toxin KP4
Killer protein 4
Gene names
Name:M2A
OrganismUstilago maydis P4 virus (UmV4) (UmV-P4)
Taxonomic identifier11009 [NCBI]
Taxonomic lineageVirusesdsRNA virusesTotiviridaeTotivirus
Virus hostUstilago maydis (Corn smut fungus) [TaxID: 5270]

Protein attributes

Sequence length127 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This protein is lethal to sensitive cells of the same or related species. It specifically inhibits voltage-gated calcium channels. It inhibits cell growth and division by blocking calcium import. Ref.4

Subunit structure

Monomer.

Subcellular location

Secreted.

Biotechnological use

The KP4 toxin expressed in transgenic plant can render them resistant to KP4-susceptible fungal pathogens such as grass smut fungi. Successful tests have been made in tobacco and wheat.

Caution

Was originally (Ref.3) thought to be glycosylated, but this does not seem to be the case according to Ref.1.

Mass spectrometry

Molecular mass is 11045±11 Da from positions 23 - 127. Determined by MALDI. Ref.1

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionToxin
   PTMDisulfide bond
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Biological_processpathogenesis

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Ref.1 Ref.3
Chain23 – 127105KP4 killer toxin
PRO_0000041338

Amino acid modifications

Disulfide bond27 ↔ 100
Disulfide bond33 ↔ 103
Disulfide bond49 ↔ 89
Disulfide bond57 ↔ 82
Disulfide bond66 ↔ 127

Experimental info

Sequence conflict561W → I AA sequence Ref.1
Sequence conflict661C → E AA sequence Ref.1
Sequence conflict671G → C AA sequence Ref.1
Sequence conflict731S → D AA sequence Ref.1
Sequence conflict741A → H AA sequence Ref.1
Sequence conflict791T → S AA sequence Ref.1

Secondary structure

................... 127
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q90121 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 34CAA729C9A63E1E

FASTA12713,489
        10         20         30         40         50         60 
MQIINVVYSF LFAAAMLPVV HSLGINCRGS SQCGLSGGNL MVRIRDQACG NQGQTWCPGE 

        70         80         90        100        110        120 
RRAKVCGTGN SISAYVQSTN NCISGTEACR HLTNLVNHGC RVCGSDPLYA GNDVSRGQLT 


VNYVNSC 

« Hide

References

[1]"Structure and heterologous expression of the Ustilago maydis viral toxin KP4."
Park C.-M., Bruenn J.A., Ganesa C., Flurkey W.F., Bozarth R.F., Koltin Y.
Mol. Microbiol. 11:155-164(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], PROTEIN SEQUENCE OF 23-79 AND 124-127, MASS SPECTROMETRY.
Strain: 77.
[2]Gu F., Khimani A.K., Flurkey W.F., Bozarth R.F., Smith T.J., Rane S.
Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: M2B.
[3]"Ustilago maydis virus P4 killer toxin: characterization, partial amino terminus sequence, and evidence for glycosylation."
Ganesa C., Flurkey W.H., Randhawa Z.I., Bozarth R.F.
Arch. Biochem. Biophys. 286:195-200(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, PROTEIN SEQUENCE OF 23-52.
[4]"KP4 fungal toxin inhibits growth in Ustilago maydis by blocking calcium uptake."
Gage M.J., Bruenn J., Fischer M., Sanders D., Smith T.J.
Mol. Microbiol. 41:775-785(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Structure and function of a virally encoded fungal toxin from Ustilago maydis: a fungal and mammalian Ca2+ channel inhibitor."
Gu F., Khimani A., Rane S.G., Flurkey W.H., Bozarth R.F., Smith T.J.
Structure 3:805-814(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L12226 Genomic RNA. Translation: AAA89185.1.
U25179 Genomic RNA. Translation: AAA75041.1.
PIRS40034.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1KPTX-ray1.75A/B23-127[»]
ProteinModelPortalQ90121.
SMRQ90121. Positions 23-127.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.430.10. 1 hit.
InterProIPR015131. Killer_tox_Kp4.
IPR011329. Killer_tox_Kp4/SMK.
[Graphical view]
PfamPF09044. Kp4. 1 hit.
[Graphical view]
SUPFAMSSF55221. SSF55221. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ90121.

Entry information

Entry nameKP4T_UMV4
AccessionPrimary (citable) accession number: Q90121
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: October 16, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references