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Q90121

- KP4T_UMV4

UniProt

Q90121 - KP4T_UMV4

Protein

KP4 killer toxin

Gene

M2A

Organism
Ustilago maydis P4 virus (UmV4) (UmV-P4)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    This protein is lethal to sensitive cells of the same or related species. It specifically inhibits voltage-gated calcium channels. It inhibits cell growth and division by blocking calcium import.1 Publication

    GO - Biological processi

    1. pathogenesis Source: InterPro

    Keywords - Molecular functioni

    Toxin

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    KP4 killer toxin
    Alternative name(s):
    Fungal toxin KP4
    Killer protein 4
    Gene namesi
    Name:M2A
    OrganismiUstilago maydis P4 virus (UmV4) (UmV-P4)
    Taxonomic identifieri11009 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesTotiviridaeTotivirus
    Virus hostiUstilago maydis (Corn smut fungus) [TaxID: 5270]

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Biotechnological usei

    The KP4 toxin expressed in transgenic plant can render them resistant to KP4-susceptible fungal pathogens such as grass smut fungi. Successful tests have been made in tobacco and wheat.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 22222 PublicationsAdd
    BLAST
    Chaini23 – 127105KP4 killer toxinPRO_0000041338Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi27 ↔ 100
    Disulfide bondi33 ↔ 103
    Disulfide bondi49 ↔ 89
    Disulfide bondi57 ↔ 82
    Disulfide bondi66 ↔ 127

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    Secondary structure

    1
    127
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi31 – 355
    Helixi40 – 5011
    Beta strandi54 – 563
    Beta strandi63 – 664
    Beta strandi71 – 8010
    Helixi85 – 9814
    Beta strandi104 – 1096
    Helixi114 – 1163
    Beta strandi118 – 1258

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1KPTX-ray1.75A/B23-127[»]
    ProteinModelPortaliQ90121.
    SMRiQ90121. Positions 23-127.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ90121.

    Family & Domainsi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.30.430.10. 1 hit.
    InterProiIPR015131. Killer_tox_Kp4.
    IPR011329. Killer_tox_Kp4/SMK.
    [Graphical view]
    PfamiPF09044. Kp4. 1 hit.
    [Graphical view]
    SUPFAMiSSF55221. SSF55221. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q90121-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQIINVVYSF LFAAAMLPVV HSLGINCRGS SQCGLSGGNL MVRIRDQACG    50
    NQGQTWCPGE RRAKVCGTGN SISAYVQSTN NCISGTEACR HLTNLVNHGC 100
    RVCGSDPLYA GNDVSRGQLT VNYVNSC 127
    Length:127
    Mass (Da):13,489
    Last modified:November 1, 1996 - v1
    Checksum:i34CAA729C9A63E1E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti56 – 561W → I AA sequence (PubMed:8145639)Curated
    Sequence conflicti66 – 661C → E AA sequence (PubMed:8145639)Curated
    Sequence conflicti67 – 671G → C AA sequence (PubMed:8145639)Curated
    Sequence conflicti73 – 731S → D AA sequence (PubMed:8145639)Curated
    Sequence conflicti74 – 741A → H AA sequence (PubMed:8145639)Curated
    Sequence conflicti79 – 791T → S AA sequence (PubMed:8145639)Curated

    Mass spectrometryi

    Molecular mass is 11045±11 Da from positions 23 - 127. Determined by MALDI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L12226 Genomic RNA. Translation: AAA89185.1.
    U25179 Genomic RNA. Translation: AAA75041.1.
    PIRiS40034.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L12226 Genomic RNA. Translation: AAA89185.1 .
    U25179 Genomic RNA. Translation: AAA75041.1 .
    PIRi S40034.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1KPT X-ray 1.75 A/B 23-127 [» ]
    ProteinModelPortali Q90121.
    SMRi Q90121. Positions 23-127.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q90121.

    Family and domain databases

    Gene3Di 3.30.430.10. 1 hit.
    InterProi IPR015131. Killer_tox_Kp4.
    IPR011329. Killer_tox_Kp4/SMK.
    [Graphical view ]
    Pfami PF09044. Kp4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55221. SSF55221. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Structure and heterologous expression of the Ustilago maydis viral toxin KP4."
      Park C.-M., Bruenn J.A., Ganesa C., Flurkey W.F., Bozarth R.F., Koltin Y.
      Mol. Microbiol. 11:155-164(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], PROTEIN SEQUENCE OF 23-79 AND 124-127, MASS SPECTROMETRY.
      Strain: 77.
    2. Gu F., Khimani A.K., Flurkey W.F., Bozarth R.F., Smith T.J., Rane S.
      Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: M2B.
    3. "Ustilago maydis virus P4 killer toxin: characterization, partial amino terminus sequence, and evidence for glycosylation."
      Ganesa C., Flurkey W.H., Randhawa Z.I., Bozarth R.F.
      Arch. Biochem. Biophys. 286:195-200(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, PROTEIN SEQUENCE OF 23-52.
    4. "KP4 fungal toxin inhibits growth in Ustilago maydis by blocking calcium uptake."
      Gage M.J., Bruenn J., Fischer M., Sanders D., Smith T.J.
      Mol. Microbiol. 41:775-785(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Structure and function of a virally encoded fungal toxin from Ustilago maydis: a fungal and mammalian Ca2+ channel inhibitor."
      Gu F., Khimani A., Rane S.G., Flurkey W.H., Bozarth R.F., Smith T.J.
      Structure 3:805-814(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).

    Entry informationi

    Entry nameiKP4T_UMV4
    AccessioniPrimary (citable) accession number: Q90121
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Caution

    Was originally thought to be glycosylated, but this does not seem to be the case according to PubMed:8145639.1 Publication

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3