Q8ZZJ7 (PURL_PYRAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	PAE0225

Organism

Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)

Taxonomic identifier

178306 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum

Protein attributes

Sequence length	697 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 697	697	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_0000100520
Regions
	Nucleotide binding	91 – 102	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8ZZJ7 [UniParc]. Last modified March 5, 2002. Version 1. Checksum: 94219332A19B8558	FASTA	697	74,373
	10 20 30 40 50 60 MALSPGELEL IRSALGREPT EVEYALFRSH WSEHCSYKST RMWLRRLPSK APWVVRGPGT 70 80 90 100 110 120 DAPLVEIAEG LYATFKIESH NHPSAVDPYN GAATGVGGII RDILTVGARP IALLVNLHFG 130 140 150 160 170 180 PPSHPHARWI AVNVIRGISD YGNRVGVPVV GGETWFHEDF TYTPIVLATC VGIVEKDGVP 190 200 210 220 230 240 PGGAEPGDLI IVAGLGADKS GLGGSAFASK TLSEEAEEDL GAVQVADPLM GKKLIDLVRE 250 260 270 280 290 300 ARSCVKFIKD LGGGGLATAL AELAEWFQLG VVAELDKIHM ADREMGPAEV LTSETQERMV 310 320 330 340 350 360 FVTSPSKLEC LERLLEKFEV PYSIVGRFTA EGRVVLKWRG EVVGDLPLSL VAKAPETPWP 370 380 390 400 410 420 QEPYAPPPLP HVPEPPIEKA IDAVLSSPNV AFKQAIYERF DFDVGVRTVL KPGEGDAAVL 430 440 450 460 470 480 KIYERGNLGL AVKGDANPRF TYLNPRLGAA NAFIKAYRNV AVVGGRPLAA VDSINVGSPS 490 500 510 520 530 540 RPSAYWQFVE AVEGLREAAE ALGVPIVGGK VSLYNEYKGK PVAPTVAVVV LGVVEDVSKV 550 560 570 580 590 600 NKAVWKEGDG VYVWGVTKDE VGGSEYLHRV HGLVAGQPPS IDYSVEKELA AVVRKWAGRL 610 620 630 640 650 660 TGAKDVGLGG LAAALAKMAA ASGIGADIDI CKAPSTTARL DYLLFSESNG RFIAAGEEGP 670 680 690 GTRVGAAGGD YFTLRCGSTI VYKRKVEELA ELMSLKI

References

[1]

"Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum." Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I., Miller J.H. Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002) [PubMed: 11792869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009441 Genomic DNA. Translation: AAL62642.1.
RefSeq	NP_558460.1. NC_003364.1.
3D structure databases
ProteinModelPortal	Q8ZZJ7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1464860.
GenomeReviews	Gene locus PAE0225 in contig AE009441_GR.
KEGG	pai:PAE0225.
NMPDR	fig|178306.1.peg.150.
Phylogenomic databases
HOGENOM	HBG311214.
OMA	YGNSFGV.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	PAER178306:PAE0225-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_PYRAE

Accession

Primary (citable) accession number: Q8ZZJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	March 5, 2002
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program