Q8ZYU6 (ILVD_PYRAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	PAE0615

Organism

Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)

Taxonomic identifier

178306 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 562	562	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000103548
Sites
	Metal binding	124	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	196	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8ZYU6 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 1D4DF358B14FC866	FASTA	562	59,751
	10 20 30 40 50 60 MVKIRIRSPL WYDGVDNAPH RSYLKAIGFT EEDFSKPIVG VLASWSELGP CNYHTLELAK 70 80 90 100 110 120 YVKEGIKEAG GVGLAAPTIV VNDGINMGTP GMRYSLISRD LIADTIEAQF NAHGVDAWVG 130 140 150 160 170 180 IGGCDKTQPG IMMAMVRLDI PAVYLYGGTA EAGWLGEREL TIEDTFEAVG AYLAGRITLE 190 200 210 220 230 240 DLKRIEELSF PSYGTCQGLF TANTMAMIGE ALGLSLLGSA SPPATSSRRR AFAIASGRAV 250 260 270 280 290 300 LKAAELGITP RKVVTYDALY NAAVTLFATA GSTNAILHLL AIAHEAGVKF TLDDFDEISK 310 320 330 340 350 360 KVPVIAALRP AGPYAMQDLD KIGGVPRILK KLYKAGLLRG EALTVEGETI GKLLDRWQPP 370 380 390 400 410 420 PLPEAGVLYD VDRPYKPYSG IRILRGNLAP NGAVMKVGAA ERLKFEGRAK VYDSEAEAFK 430 440 450 460 470 480 AVASGEIKAG DVVVIRYEGP KGAPGMPEML KITAAIVGAG LGEVVALITD GRFSGATRGI 490 500 510 520 530 540 MVGHVSPEAA VGGPIALVQN GDRIVIDGEA GLLKLELGEE ELERRRKNWS PPPPKYKGGL 550 560 LAKYASLVQQ ADKGAVTSPP VL

References

[1]

"Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum." Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I., Miller J.H. Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002) [PubMed: 11792869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009441 Genomic DNA. Translation: AAL62897.1.
RefSeq	NP_558715.1. NC_003364.1.
3D structure databases
ProteinModelPortal	Q8ZYU6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1465122.
GenomeReviews	Gene locus PAE0615 in contig AE009441_GR.
KEGG	pai:PAE0615.
NMPDR	fig|178306.1.peg.405.
Phylogenomic databases
HOGENOM	HBG671001.
OMA	NMPGAMI.
ProtClustDB	PRK00911.
Enzyme and pathway databases
BioCyc	PAER178306:PAE0615-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_PYRAE

Accession

Primary (citable) accession number: Q8ZYU6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	March 1, 2002
Last modified:	December 14, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program