Q8ZYT7 (SYY1_PYRAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine--tRNA ligase 1
EC=6.1.1.1

Alternative name(s):
Tyrosyl-tRNA synthetase 1
Short name=TyrRS 1

Gene names

Name:	tyrS1
Ordered Locus Names:	PAE0630

Organism

Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)

Taxonomic identifier

178306 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity. HAMAP MF_02009
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02009
Subunit structure	Homodimer By similarity. HAMAP MF_02009
Subcellular location	Cytoplasm By similarity HAMAP MF_02009.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 4 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Tyrosine--tRNA ligase 1 HAMAP MF_02009		PRO_0000240266
Regions
	Motif	246 – 250	5	"KMSKS" region HAMAP MF_02009
Sites
	Binding site	37	1	Tyrosine By similarity
	Binding site	169	1	Tyrosine By similarity
	Binding site	173	1	Tyrosine By similarity
	Binding site	176	1	Tyrosine By similarity
	Binding site	191	1	Tyrosine By similarity
	Binding site	249	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ZYT7 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 199783D4735D866A	FASTA	372	42,391
	10 20 30 40 50 60 MDVESKISLI LRYPTEEVIT VEELRELLQL GHQLNHYIGF EISGFIHIGT GIVSMSKVVD 70 80 90 100 110 120 LQRAGVRTQI LLADIHSWLN NKLGGDLDTI RKVAVTYYIE AFKKVIETLG GDPDATRFVL 130 140 150 160 170 180 GSDLYHHNDE YWLLLMDITR HLTLSQVRHS LTILGRKMGE SIPLAYLVYP PLQVADVFAL 190 200 210 220 230 240 GAHIPHGGVD QRRAHILARQ VADKIRFYPL EVGGKRVKPV ALHHKLLPAL NISTKPSNKE 250 260 270 280 290 300 ELSEMKMSKS VPQSAIFIHD SPEEIRQKIS KAYCPPRETE YNPVLELLHI SAFREERKTP 310 320 330 340 350 360 FIIKRPPQYG GDIEVWTYEE VERLYREGKI HPADLKNATA EALINILEPI YKYFQGPGAK 370 LLQEMKNITI TR

References

[1]

"Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum." Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I., Miller J.H. Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002) [PubMed: 11792869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009441 Genomic DNA. Translation: AAL62906.1.
RefSeq	NP_558724.1. NC_003364.1.
3D structure databases
ProteinModelPortal	Q8ZYT7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1465131.
GenomeReviews	Gene locus PAE0630 in contig AE009441_GR.
KEGG	pai:PAE0630.
NMPDR	fig|178306.1.peg.414.
Phylogenomic databases
HOGENOM	HBG330667.
OMA	YIGFEIS.
ProtClustDB	PRK08560.
Enzyme and pathway databases
BioCyc	PAER178306:PAE0630-MONOMER.
Family and domain databases
HAMAP	MF_02009. Tyr_tRNA_synth_type4. [Tree]
InterPro	IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR023678. Tyr-tRNA-synth_4. IPR023617. Tyr-tRNA-synth_arc-type. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01866.
PANTHER	PTHR11946:SF8. Tyr-tRNA_synth. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PIRSF	PIRSF006588. TyrRS_arch_euk. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY1_PYRAE

Accession

Primary (citable) accession number: Q8ZYT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program