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Q8ZYE0

- KPYK_PYRAE

UniProt

Q8ZYE0 - KPYK_PYRAE

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Protein

Pyruvate kinase

Gene

pyk

Organism
Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Divalent metal cations.1 Publication

Enzyme regulationi

Not activated by classical allosteric effectors.

Kineticsi

    Vmax=46 µmol/min/mg enzyme (at 65 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 6.0.1 Publication

    Temperature dependencei

    Optimum temperature is higher than 98 degrees Celsius. Thermostable for 2 hours up to 100 degrees Celsius.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei46 – 461SubstrateBy similarity
    Metal bindingi48 – 481PotassiumBy similarity
    Metal bindingi80 – 801PotassiumBy similarity
    Sitei230 – 2301Transition state stabilizerBy similarity
    Metal bindingi232 – 2321MagnesiumBy similarity
    Binding sitei255 – 2551Substrate; via amide nitrogenBy similarity
    Metal bindingi256 – 2561MagnesiumBy similarity
    Binding sitei256 – 2561Substrate; via amide nitrogenBy similarity
    Binding sitei288 – 2881SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway
    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    BioCyciPAER178306:GCEO-575-MONOMER.
    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase (EC:2.7.1.40)
    Short name:
    PK
    Gene namesi
    Name:pyk
    Ordered Locus Names:PAE0819
    OrganismiPyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)
    Taxonomic identifieri178306 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum
    ProteomesiUP000002439: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 461461Pyruvate kinasePRO_0000295181Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    STRINGi178306.PAE0819.

    Structurei

    Secondary structure

    1
    461
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi16 – 227
    Helixi25 – 284
    Helixi32 – 398
    Beta strandi43 – 486
    Turni49 – 513
    Helixi54 – 7118
    Beta strandi76 – 805
    Beta strandi94 – 963
    Beta strandi101 – 1066
    Beta strandi112 – 1176
    Helixi120 – 1256
    Beta strandi131 – 1344
    Helixi135 – 1373
    Beta strandi139 – 1468
    Beta strandi148 – 15710
    Beta strandi159 – 1613
    Beta strandi166 – 1694
    Helixi181 – 19010
    Helixi191 – 1966
    Beta strandi199 – 2024
    Helixi208 – 22013
    Beta strandi226 – 2316
    Helixi234 – 2385
    Helixi240 – 2456
    Beta strandi248 – 2536
    Helixi254 – 2574
    Turni258 – 2603
    Turni263 – 2653
    Helixi266 – 27914
    Beta strandi284 – 2918
    Helixi292 – 2954
    Helixi302 – 31312
    Beta strandi317 – 3215
    Helixi323 – 3264
    Helixi331 – 34313
    Helixi358 – 37316
    Beta strandi376 – 3805
    Beta strandi382 – 3843
    Helixi385 – 3917
    Beta strandi399 – 4046
    Helixi406 – 4127
    Beta strandi418 – 4225
    Helixi428 – 43912
    Beta strandi442 – 4487
    Beta strandi456 – 4605

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3QTGX-ray2.20A/B1-461[»]
    ProteinModelPortaliQ8ZYE0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0469.
    HOGENOMiHOG000021558.
    InParanoidiQ8ZYE0.
    KOiK00873.
    OMAiREMATIC.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8ZYE0-1 [UniParc]FASTAAdd to Basket

    « Hide

            10         20         30         40         50
    MSAPRGDHAI LRARNLTKRV ATLGPSTDVL RPDELIKFLD LVDGVRINLA
    60 70 80 90 100
    HASPNEVKFR IEAVRSYEKA KNRPLAVIVD LKGPSIRVGS TSPINVQEGE
    110 120 130 140 150
    VVKFKLSDKS DGTYIPVPNK AFFSAVEQND VILMLDGRLR LKVTNTGSDW
    160 170 180 190 200
    IEAVAESSGV ITGGKAIVVE GKDYDISTPA EEDVEALKAI SPIRDNIDYV
    210 220 230 240 250
    AISLAKSCKD VDSVRSLLTE LGFQSQVAVK IETKGAVNNL EELVQCSDYV
    260 270 280 290 300
    VVARGDLGLH YGLDALPIVQ RRIVHTSLKY GKPIAVATQL LDSMQSSPIP
    310 320 330 340 350
    TRAEINDVFT TASMGVDSLW LTNETASGKY PLAAVSWLSR ILMNVEYQIP
    360 370 380 390 400
    QSPLLQNSRD RFAKGLVELA QDLGANILVF SMSGTLARRI AKFRPRGVVY
    410 420 430 440 450
    VGTPNVRVAR SLSIVWALEP LYIPAENYEE GLEKLISLKG TTPFVATYGI
    460
    RGGVHSVKVK L
    Length:461
    Mass (Da):50,262
    Last modified:March 1, 2002 - v1
    Checksum:iD43333DA69BDE14C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE009441 Genomic DNA. Translation: AAL63053.1.
    RefSeqiNP_558871.1. NC_003364.1.

    Genome annotation databases

    EnsemblBacteriaiAAL63053; AAL63053; PAE0819.
    GeneIDi1465282.
    KEGGipai:PAE0819.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE009441 Genomic DNA. Translation: AAL63053.1 .
    RefSeqi NP_558871.1. NC_003364.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3QTG X-ray 2.20 A/B 1-461 [» ]
    ProteinModelPortali Q8ZYE0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 178306.PAE0819.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL63053 ; AAL63053 ; PAE0819 .
    GeneIDi 1465282.
    KEGGi pai:PAE0819.

    Phylogenomic databases

    eggNOGi COG0469.
    HOGENOMi HOG000021558.
    InParanoidi Q8ZYE0.
    KOi K00873.
    OMAi REMATIC.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .
    BioCyci PAER178306:GCEO-575-MONOMER.

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. "Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum."
      Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I., Miller J.H.
      Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827.
    2. "Comparative analysis of pyruvate kinases from the hyperthermophilic archaea Archaeoglobus fulgidus, Aeropyrum pernix, and Pyrobaculum aerophilum and the hyperthermophilic bacterium Thermotoga maritima: unusual regulatory properties in hyperthermophilic archaea."
      Johnsen U., Hansen T., Schoenheit P.
      J. Biol. Chem. 278:25417-25427(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, SUBUNIT, REGULATION.
      Strain: ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827.

    Entry informationi

    Entry nameiKPYK_PYRAE
    AccessioniPrimary (citable) accession number: Q8ZYE0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 10, 2007
    Last sequence update: March 1, 2002
    Last modified: October 29, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3