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Q8ZYE0

- KPYK_PYRAE

UniProt

Q8ZYE0 - KPYK_PYRAE

Protein

Pyruvate kinase

Gene

pyk

Organism
Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Divalent metal cations.1 Publication

    Enzyme regulationi

    Not activated by classical allosteric effectors.

    Kineticsi

      Vmax=46 µmol/min/mg enzyme (at 65 degrees Celsius)1 Publication

      pH dependencei

      Optimum pH is 6.0.1 Publication

      Temperature dependencei

      Optimum temperature is higher than 98 degrees Celsius. Thermostable for 2 hours up to 100 degrees Celsius.1 Publication

      Pathwayi

      Sites

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Binding sitei46 – 461SubstrateBy similarity
      Metal bindingi48 – 481PotassiumBy similarity
      Metal bindingi80 – 801PotassiumBy similarity
      Sitei230 – 2301Transition state stabilizerBy similarity
      Metal bindingi232 – 2321MagnesiumBy similarity
      Binding sitei255 – 2551Substrate; via amide nitrogenBy similarity
      Metal bindingi256 – 2561MagnesiumBy similarity
      Binding sitei256 – 2561Substrate; via amide nitrogenBy similarity
      Binding sitei288 – 2881SubstrateBy similarity

      GO - Molecular functioni

      1. ATP binding Source: UniProtKB-KW
      2. magnesium ion binding Source: InterPro
      3. potassium ion binding Source: InterPro
      4. pyruvate kinase activity Source: UniProtKB-EC

      GO - Biological processi

      1. glycolytic process Source: UniProtKB-UniPathway

      Keywords - Molecular functioni

      Kinase, Transferase

      Keywords - Biological processi

      Glycolysis

      Keywords - Ligandi

      ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

      Enzyme and pathway databases

      BioCyciPAER178306:GCEO-575-MONOMER.
      UniPathwayiUPA00109; UER00188.

      Names & Taxonomyi

      Protein namesi
      Recommended name:
      Pyruvate kinase (EC:2.7.1.40)
      Short name:
      PK
      Gene namesi
      Name:pyk
      Ordered Locus Names:PAE0819
      OrganismiPyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)
      Taxonomic identifieri178306 [NCBI]
      Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum
      ProteomesiUP000002439: Chromosome

      PTM / Processingi

      Molecule processing

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Chaini1 – 461461Pyruvate kinasePRO_0000295181Add
      BLAST

      Interactioni

      Subunit structurei

      Homotetramer.1 Publication

      Protein-protein interaction databases

      STRINGi178306.PAE0819.

      Structurei

      Secondary structure

      1
      461
      Legend: HelixTurnBeta strand
      Show more details
      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Beta strandi16 – 227
      Helixi25 – 284
      Helixi32 – 398
      Beta strandi43 – 486
      Turni49 – 513
      Helixi54 – 7118
      Beta strandi76 – 805
      Beta strandi94 – 963
      Beta strandi101 – 1066
      Beta strandi112 – 1176
      Helixi120 – 1256
      Beta strandi131 – 1344
      Helixi135 – 1373
      Beta strandi139 – 1468
      Beta strandi148 – 15710
      Beta strandi159 – 1613
      Beta strandi166 – 1694
      Helixi181 – 19010
      Helixi191 – 1966
      Beta strandi199 – 2024
      Helixi208 – 22013
      Beta strandi226 – 2316
      Helixi234 – 2385
      Helixi240 – 2456
      Beta strandi248 – 2536
      Helixi254 – 2574
      Turni258 – 2603
      Turni263 – 2653
      Helixi266 – 27914
      Beta strandi284 – 2918
      Helixi292 – 2954
      Helixi302 – 31312
      Beta strandi317 – 3215
      Helixi323 – 3264
      Helixi331 – 34313
      Helixi358 – 37316
      Beta strandi376 – 3805
      Beta strandi382 – 3843
      Helixi385 – 3917
      Beta strandi399 – 4046
      Helixi406 – 4127
      Beta strandi418 – 4225
      Helixi428 – 43912
      Beta strandi442 – 4487
      Beta strandi456 – 4605

      3D structure databases

      Select the link destinations:
      PDBe
      RCSB PDB
      PDBj
      Links Updated
      EntryMethodResolution (Å)ChainPositionsPDBsum
      3QTGX-ray2.20A/B1-461[»]
      ProteinModelPortaliQ8ZYE0.
      ModBaseiSearch...
      MobiDBiSearch...

      Family & Domainsi

      Sequence similaritiesi

      Belongs to the pyruvate kinase family.Curated

      Phylogenomic databases

      eggNOGiCOG0469.
      HOGENOMiHOG000021558.
      KOiK00873.
      OMAiREMATIC.

      Family and domain databases

      Gene3Di2.40.33.10. 1 hit.
      3.20.20.60. 2 hits.
      3.40.1380.20. 1 hit.
      InterProiIPR001697. Pyr_Knase.
      IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
      IPR011037. Pyrv_Knase-like_insert_dom.
      IPR015794. Pyrv_Knase_a/b.
      IPR015793. Pyrv_Knase_brl.
      IPR015795. Pyrv_Knase_C.
      IPR015806. Pyrv_Knase_insert_dom.
      [Graphical view]
      PANTHERiPTHR11817. PTHR11817. 1 hit.
      PfamiPF00224. PK. 1 hit.
      PF02887. PK_C. 1 hit.
      [Graphical view]
      PRINTSiPR01050. PYRUVTKNASE.
      SUPFAMiSSF50800. SSF50800. 1 hit.
      SSF51621. SSF51621. 2 hits.
      SSF52935. SSF52935. 1 hit.
      TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.

      Sequencei

      Sequence statusi: Complete.

      Q8ZYE0-1 [UniParc]FASTAAdd to Basket

      « Hide

      MSAPRGDHAI LRARNLTKRV ATLGPSTDVL RPDELIKFLD LVDGVRINLA    50
      HASPNEVKFR IEAVRSYEKA KNRPLAVIVD LKGPSIRVGS TSPINVQEGE 100
      VVKFKLSDKS DGTYIPVPNK AFFSAVEQND VILMLDGRLR LKVTNTGSDW 150
      IEAVAESSGV ITGGKAIVVE GKDYDISTPA EEDVEALKAI SPIRDNIDYV 200
      AISLAKSCKD VDSVRSLLTE LGFQSQVAVK IETKGAVNNL EELVQCSDYV 250
      VVARGDLGLH YGLDALPIVQ RRIVHTSLKY GKPIAVATQL LDSMQSSPIP 300
      TRAEINDVFT TASMGVDSLW LTNETASGKY PLAAVSWLSR ILMNVEYQIP 350
      QSPLLQNSRD RFAKGLVELA QDLGANILVF SMSGTLARRI AKFRPRGVVY 400
      VGTPNVRVAR SLSIVWALEP LYIPAENYEE GLEKLISLKG TTPFVATYGI 450
      RGGVHSVKVK L 461
      Length:461
      Mass (Da):50,262
      Last modified:March 1, 2002 - v1
      Checksum:iD43333DA69BDE14C
      GO

      Sequence databases

      Select the link destinations:
      EMBL
      GenBank
      DDBJ
      Links Updated
      AE009441 Genomic DNA. Translation: AAL63053.1.
      RefSeqiNP_558871.1. NC_003364.1.

      Genome annotation databases

      EnsemblBacteriaiAAL63053; AAL63053; PAE0819.
      GeneIDi1465282.
      KEGGipai:PAE0819.

      Cross-referencesi

      Sequence databases

      Select the link destinations:
      EMBL
      GenBank
      DDBJ
      Links Updated
      AE009441 Genomic DNA. Translation: AAL63053.1 .
      RefSeqi NP_558871.1. NC_003364.1.

      3D structure databases

      Select the link destinations:
      PDBe
      RCSB PDB
      PDBj
      Links Updated
      Entry Method Resolution (Å) Chain Positions PDBsum
      3QTG X-ray 2.20 A/B 1-461 [» ]
      ProteinModelPortali Q8ZYE0.
      ModBasei Search...
      MobiDBi Search...

      Protein-protein interaction databases

      STRINGi 178306.PAE0819.

      Protocols and materials databases

      Structural Biology Knowledgebase Search...

      Genome annotation databases

      EnsemblBacteriai AAL63053 ; AAL63053 ; PAE0819 .
      GeneIDi 1465282.
      KEGGi pai:PAE0819.

      Phylogenomic databases

      eggNOGi COG0469.
      HOGENOMi HOG000021558.
      KOi K00873.
      OMAi REMATIC.

      Enzyme and pathway databases

      UniPathwayi UPA00109 ; UER00188 .
      BioCyci PAER178306:GCEO-575-MONOMER.

      Family and domain databases

      Gene3Di 2.40.33.10. 1 hit.
      3.20.20.60. 2 hits.
      3.40.1380.20. 1 hit.
      InterProi IPR001697. Pyr_Knase.
      IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
      IPR011037. Pyrv_Knase-like_insert_dom.
      IPR015794. Pyrv_Knase_a/b.
      IPR015793. Pyrv_Knase_brl.
      IPR015795. Pyrv_Knase_C.
      IPR015806. Pyrv_Knase_insert_dom.
      [Graphical view ]
      PANTHERi PTHR11817. PTHR11817. 1 hit.
      Pfami PF00224. PK. 1 hit.
      PF02887. PK_C. 1 hit.
      [Graphical view ]
      PRINTSi PR01050. PYRUVTKNASE.
      SUPFAMi SSF50800. SSF50800. 1 hit.
      SSF51621. SSF51621. 2 hits.
      SSF52935. SSF52935. 1 hit.
      TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
      ProtoNeti Search...

      Publicationsi

      1. "Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum."
        Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I., Miller J.H.
        Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002) [PubMed] [Europe PMC] [Abstract]
        Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
        Strain: ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827.
      2. "Comparative analysis of pyruvate kinases from the hyperthermophilic archaea Archaeoglobus fulgidus, Aeropyrum pernix, and Pyrobaculum aerophilum and the hyperthermophilic bacterium Thermotoga maritima: unusual regulatory properties in hyperthermophilic archaea."
        Johnsen U., Hansen T., Schoenheit P.
        J. Biol. Chem. 278:25417-25427(2003) [PubMed] [Europe PMC] [Abstract]
        Cited for: BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, SUBUNIT, REGULATION.
        Strain: ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827.

      Entry informationi

      Entry nameiKPYK_PYRAE
      AccessioniPrimary (citable) accession number: Q8ZYE0
      Entry historyi
      Integrated into UniProtKB/Swiss-Prot: July 10, 2007
      Last sequence update: March 1, 2002
      Last modified: October 1, 2014
      This is version 72 of the entry and version 1 of the sequence. [Complete history]
      Entry statusiReviewed (UniProtKB/Swiss-Prot)
      Annotation programProkaryotic Protein Annotation Program

      Miscellaneousi

      Keywords - Technical termi

      3D-structure, Complete proteome, Reference proteome

      Documents

      1. PATHWAY comments
        Index of metabolic and biosynthesis pathways
      2. PDB cross-references
        Index of Protein Data Bank (PDB) cross-references
      3. SIMILARITY comments
        Index of protein domains and families

      External Data

      Dasty 3