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Q8ZXT6 (SYL_PYRAE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:PAE1107
OrganismPyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827) [Reference proteome] [HAMAP]
Taxonomic identifier178306 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum

Protein attributes

Sequence length945 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 945945Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152138

Regions

Motif43 – 5311"HIGH" region HAMAP-Rule MF_00049
Motif638 – 6425"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6411ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8ZXT6 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 2ECDFFE9EE85F243

FASTA945106,850
        10         20         30         40         50         60 
MSELSSLFIK MAEKWQAKWA EARVYEPEPR PGAAKFFVTA AYPYPNGAIH IGHGRTYLIA 

        70         80         90        100        110        120 
DVLARFHRHM GRVVLFPMGF HYTGTPILTI AEAIASGDAT VIEEYMAIYG VPKDEIEKMG 

       130        140        150        160        170        180 
NPLYLARYFH EQSKRAMQKF GLGIDWTREF TTIDPEYQRF IQWQFEKLRE RGLIVRGRHP 

       190        200        210        220        230        240 
VGWCPRHSMP VGAHDTKDDK EPEIGQWTLI YFADGEGLVF PAATLRPETV LGVTNMWINP 

       250        260        270        280        290        300 
DAEYAVIEHN GRKMVVSKDA AFRLSFQGDV RILREARGRE FVGRSVQNPV TGEWVPVYEA 

       310        320        330        340        350        360 
KFVDPKVGTG VVMSVPAHAP YDYAALRDLN AVRLIPLIRV EGYGDYPAKE VVERMGIKSQ 

       370        380        390        400        410        420 
TDPALEEATR EVYSAEYTKG VMREDVVGRV GAHLSEPARS MLRAVFKMYF AGRPVREARE 

       430        440        450        460        470        480 
FISKWLVEAG IGGVMYDIMN KPVYCRCGTE IVVKVLEDQW FINYGEGRWK ELARKLVEEM 

       490        500        510        520        530        540 
AIVPPEAKAH FLATIDWLDK RACARTRGLG TPLPWSDGWV IESLSDSTIY MAFYTVIKRI 

       550        560        570        580        590        600 
RQFGIKPEQL IKEFWDYVFL GVGTPEEVAK RTGVPPEQLK AIREEFDFWY PLDSRNSGKD 

       610        620        630        640        650        660 
LIPNHLTFFI FNHVAIFPRE KWPRQIVANG WVLREGEKMS KSKRNVLPLD KAVDMYGPDP 

       670        680        690        700        710        720 
LRATLAITAE VEQDLDFRHA EAIRNAQQLM SIYSLAQRLA QTAEDRSPNW LDKWLVSEIA 

       730        740        750        760        770        780 
LVLERAREAY EKVRVRQAAV EVLYNAKSVF DQYLASVERP SKLAVEAARA WAVAMEPIAP 

       790        800        810        820        830        840 
HLAEEIWSIL GGEGLVVKAP WPQLRPDPVA LLAKRYVDMV IDDVKRIPAF GEGVKRVVIY 

       850        860        870        880        890        900 
VNPNYAWVKA ALSGDVKAVI NAGAPPQAAK RVVDIVKTLG DELRGLISAV GKFDEAEALA 

       910        920        930        940 
SYKNYMEKAL GAPMEIYNAD DPSAPDLGSK KKVALPLRPG IYIEK 

« Hide

References

[1]"Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum."
Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I., Miller J.H.
Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE009441 Genomic DNA. Translation: AAL63260.1.
RefSeqNP_559078.1. NC_003364.1.

3D structure databases

ProteinModelPortalQ8ZXT6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING178306.PAE1107.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL63260; AAL63260; PAE1107.
GeneID1465496.
KEGGpai:PAE1107.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000216620.
KOK01869.
OMAIDKWLLH.

Enzyme and pathway databases

BioCycPAER178306:GCEO-789-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_A. Leu_tRNA_synth_A.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsTIGR00395. leuS_arch. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_PYRAE
AccessionPrimary (citable) accession number: Q8ZXT6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2002
Last modified: May 14, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries