Q8ZXL4 (NADE_PYRAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	PAE1219

Organism

Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)

Taxonomic identifier

178306 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_0000152230
Regions
	Nucleotide binding	38 – 45	8	ATP By similarity
Sites
	Active site	40	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ZXL4 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: AB05D9BA99BFFA65	FASTA	267	29,517
	10 20 30 40 50 60 MFVYDVVNAL DYEKARSIIT AFISQYVQRA GSRGVVVGIS GGVDSTVAAA LAVEALGRQR 70 80 90 100 110 120 VLGLLMPSLY TPPEDLKDAL DVINALGVEW KRVDITPIYD AFVKTLPDFS QENRVAAGNI 130 140 150 160 170 180 LPRIRMTVLY YYANKYNLLV MGTGDRSELL LGYFTKYGDG GVDFLPIGSL FKLQVRELAA 190 200 210 220 230 240 RLGFADIAKK PSSPRLWQGH TAEGELGASY EVIDQVLYAV FDLKKPPEEV RGFFGEVVDI 250 260 VITRVKKNIH KLTPPAYPDI TPARRNV

References

[1]	"Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum." Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I., Miller J.H. Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002) [PubMed: 11792869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009441 Genomic DNA. Translation: AAL63333.1.
RefSeq	NP_559151.1. NC_003364.1.
3D structure databases
ProteinModelPortal	Q8ZXL4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1465571.
GenomeReviews	Gene locus PAE1219 in contig AE009441_GR.
KEGG	pai:PAE1219.
NMPDR	fig|178306.1.peg.841.
Phylogenomic databases
HOGENOM	HBG351567.
OMA	KLIVYLC.
ProtClustDB	PRK13980.
Enzyme and pathway databases
BioCyc	PAER178306:PAE1219-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_PYRAE

Accession

Primary (citable) accession number: Q8ZXL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	March 1, 2002
Last modified:	November 16, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program