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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei607ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PAE1617
OrganismiPyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)
Taxonomic identifieri178306 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum
Proteomesi
  • UP000002439 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000985871 – 981Isoleucine--tRNA ligaseAdd BLAST981

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi178306.PAE1617

Structurei

3D structure databases

ProteinModelPortaliQ8ZWU4
SMRiQ8ZWU4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi50 – 60"HIGH" regionAdd BLAST11
Motifi604 – 608"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00807 Archaea
COG0060 LUCA
HOGENOMiHOG000246403
InParanoidiQ8ZWU4
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG093Z0054

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 2 hits
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q8ZWU4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPHGDLPLPP SYRPHEVKKA VEEFWRRNRI FEKWKSWRGG PKFTFLEGPP
60 70 80 90 100
TTNGMPHVGH IRGRTYKDVV IRFYRLLGYD VWVQGGWDMQ GMPVEWEVEK
110 120 130 140 150
KLKLRSKKDI EQFGLEKFAL ECNSLVEEYL AYWREWGTKR LGLWLDLENA
160 170 180 190 200
YETRQPHYLQ YAWRIVKRAH ELGLLTEDYR VLWFCPRCET SLSDHEVALG
210 220 230 240 250
YDEREDPSIY VKFRVEGGVD EYLVIWTTTP WTIVDNEAVA VHPDYVYAKV
260 270 280 290 300
EVEVGGRREY WWLAEALVPS LMAKFGIKTW RVVETKKGVE LAGVRYIHPL
310 320 330 340 350
AEEVPERASR PHQVVTAEFV TLEQGTGLVH IAPGHGPEDF ELAKKYGLPV
360 370 380 390 400
TNSVEINGIY NELGGRYKGK HVYDVDKEVT RDLRSKGLLV FEEKIRHEYP
410 420 430 440 450
HCWRCGSKLI LRADRQWFIA ISRIRDKMYA ELQKVNVVPT KLRDRFDIFV
460 470 480 490 500
QNARDWNISR SRVWGTPLPV WRCKKDGRIL VIGSLEELKK LAKELPPVDD
510 520 530 540 550
FKLVHRPWID QVKISAHDCD EWVREPYVMD VWLDSGIAWI AAVDGENNRD
560 570 580 590 600
LWEKLFPYDF VTEGIDQTRG WFYSLLATSV LYTGRAPYKN VLIQGLILDK
610 620 630 640 650
HGQKMSKSKG NVIWAKDLFE KYGADPVRLY ILSKVAPWED LSFDPDEVKY
660 670 680 690 700
VIGDLNILWN VVKFADTYMS LDGFDAEKYP LSQWLEKGLE EDKWILSELN
710 720 730 740 750
IMISEFTNFV KNFEFHKAAA LWREFVVETL SHRYIRLLRR RVWSEEPSPD
760 770 780 790 800
KYAAYAVLHD VLKKVLILGS ILVPFITEYL WQAYVRKYEK NAPESVHLAQ
810 820 830 840 850
YPAAGSYDKE LIYAYRELFA VFSALAEARN KAGIKLRWPI REAYVNGAKY
860 870 880 890 900
AERYTELLKY LGNVKEVKVG RRPDYLCVKE GELEVCVPDK IEPELYYEAL
910 920 930 940 950
ARELIRRIQV MRKETGLEIS DEIHVVVETN SDDIKKAVEQ YRDYIARETR
960 970 980
AVKLIIDAVS QGKEWDISGE KVKIEIRKAQ A
Length:981
Mass (Da):114,759
Last modified:March 1, 2002 - v1
Checksum:i6F7A1242CC105D41
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009441 Genomic DNA Translation: AAL63605.1

Genome annotation databases

EnsemblBacteriaiAAL63605; AAL63605; PAE1617
KEGGipai:PAE1617
PATRICifig|178306.9.peg.1193

Similar proteinsi

Entry informationi

Entry nameiSYI_PYRAE
AccessioniPrimary (citable) accession number: Q8ZWU4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2002
Last modified: March 28, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health