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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei607 – 6071ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPAER178306:GCEO-1146-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PAE1617
OrganismiPyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)
Taxonomic identifieri178306 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum
ProteomesiUP000002439 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 981981Isoleucine--tRNA ligasePRO_0000098587Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi178306.PAE1617.

Structurei

3D structure databases

ProteinModelPortaliQ8ZWU4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi50 – 6011"HIGH" regionAdd
BLAST
Motifi604 – 6085"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
InParanoidiQ8ZWU4.
KOiK01870.
OMAiEGPPTTN.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8ZWU4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPHGDLPLPP SYRPHEVKKA VEEFWRRNRI FEKWKSWRGG PKFTFLEGPP
60 70 80 90 100
TTNGMPHVGH IRGRTYKDVV IRFYRLLGYD VWVQGGWDMQ GMPVEWEVEK
110 120 130 140 150
KLKLRSKKDI EQFGLEKFAL ECNSLVEEYL AYWREWGTKR LGLWLDLENA
160 170 180 190 200
YETRQPHYLQ YAWRIVKRAH ELGLLTEDYR VLWFCPRCET SLSDHEVALG
210 220 230 240 250
YDEREDPSIY VKFRVEGGVD EYLVIWTTTP WTIVDNEAVA VHPDYVYAKV
260 270 280 290 300
EVEVGGRREY WWLAEALVPS LMAKFGIKTW RVVETKKGVE LAGVRYIHPL
310 320 330 340 350
AEEVPERASR PHQVVTAEFV TLEQGTGLVH IAPGHGPEDF ELAKKYGLPV
360 370 380 390 400
TNSVEINGIY NELGGRYKGK HVYDVDKEVT RDLRSKGLLV FEEKIRHEYP
410 420 430 440 450
HCWRCGSKLI LRADRQWFIA ISRIRDKMYA ELQKVNVVPT KLRDRFDIFV
460 470 480 490 500
QNARDWNISR SRVWGTPLPV WRCKKDGRIL VIGSLEELKK LAKELPPVDD
510 520 530 540 550
FKLVHRPWID QVKISAHDCD EWVREPYVMD VWLDSGIAWI AAVDGENNRD
560 570 580 590 600
LWEKLFPYDF VTEGIDQTRG WFYSLLATSV LYTGRAPYKN VLIQGLILDK
610 620 630 640 650
HGQKMSKSKG NVIWAKDLFE KYGADPVRLY ILSKVAPWED LSFDPDEVKY
660 670 680 690 700
VIGDLNILWN VVKFADTYMS LDGFDAEKYP LSQWLEKGLE EDKWILSELN
710 720 730 740 750
IMISEFTNFV KNFEFHKAAA LWREFVVETL SHRYIRLLRR RVWSEEPSPD
760 770 780 790 800
KYAAYAVLHD VLKKVLILGS ILVPFITEYL WQAYVRKYEK NAPESVHLAQ
810 820 830 840 850
YPAAGSYDKE LIYAYRELFA VFSALAEARN KAGIKLRWPI REAYVNGAKY
860 870 880 890 900
AERYTELLKY LGNVKEVKVG RRPDYLCVKE GELEVCVPDK IEPELYYEAL
910 920 930 940 950
ARELIRRIQV MRKETGLEIS DEIHVVVETN SDDIKKAVEQ YRDYIARETR
960 970 980
AVKLIIDAVS QGKEWDISGE KVKIEIRKAQ A
Length:981
Mass (Da):114,759
Last modified:March 1, 2002 - v1
Checksum:i6F7A1242CC105D41
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009441 Genomic DNA. Translation: AAL63605.1.
RefSeqiNP_559423.1. NC_003364.1.
WP_011008078.1. NC_003364.1.

Genome annotation databases

EnsemblBacteriaiAAL63605; AAL63605; PAE1617.
GeneIDi1465853.
KEGGipai:PAE1617.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009441 Genomic DNA. Translation: AAL63605.1.
RefSeqiNP_559423.1. NC_003364.1.
WP_011008078.1. NC_003364.1.

3D structure databases

ProteinModelPortaliQ8ZWU4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi178306.PAE1617.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL63605; AAL63605; PAE1617.
GeneIDi1465853.
KEGGipai:PAE1617.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
InParanoidiQ8ZWU4.
KOiK01870.
OMAiEGPPTTN.

Enzyme and pathway databases

BioCyciPAER178306:GCEO-1146-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum."
    Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I., Miller J.H.
    Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827.

Entry informationi

Entry nameiSYI_PYRAE
AccessioniPrimary (citable) accession number: Q8ZWU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2002
Last modified: July 22, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.