ID   TRM1_PYRAE              Reviewed;         363 AA.
AC   Q8ZWT5;
DT   20-JUN-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   05-MAY-2009, entry version 32.
DE   RecName: Full=N(2),N(2)-dimethylguanosine tRNA methyltransferase;
DE            EC=2.1.1.32;
DE   AltName: Full=tRNA(guanine-26,N(2)-N(2)) methyltransferase;
DE   AltName: Full=tRNA 2,2-dimethylguanosine-26 methyltransferase;
DE   AltName: Full=tRNA(m(2,2)G26)dimethyltransferase;
GN   Name=trm1; OrderedLocusNames=PAE1628;
OS   Pyrobaculum aerophilum.
OC   Archaea; Crenarchaeota; Thermoprotei; Thermoproteales;
OC   Thermoproteaceae; Pyrobaculum.
OX   NCBI_TaxID=13773;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827;
RX   MEDLINE=21664397; PubMed=11792869; DOI=10.1073/pnas.241636498;
RA   Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I.,
RA   Miller J.H.;
RT   "Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum
RT   aerophilum.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002).
CC   -!- FUNCTION: Dimethylates a single guanine residue at position 26 of
CC       a number of tRNAs using S-adenosyl-L-methionine as donor of the
CC       methyl groups (By similarity).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-
CC       homocysteine + tRNA containing N(2)-methylguanine.
CC   -!- SIMILARITY: Belongs to the TRM1 family.
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DR   EMBL; AE009831; AAL63614.1; -; Genomic_DNA.
DR   RefSeq; NP_559432.1; -.
DR   GeneID; 1465863; -.
DR   GenomeReviews; AE009441_GR; PAE1628.
DR   KEGG; pai:PAE1628; -.
DR   NMPDR; fig|178306.1.peg.1122; -.
DR   HOGENOM; Q8ZWT5; -.
DR   OMA; Q8ZWT5; FHEDANV.
DR   BioCyc; PAER178306:PAE1628-MON; -.
DR   BRENDA; 2.1.1.32; 142830.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0004809; F:tRNA (guanine-N2-)-methyltransferase activity; IEA:HAMAP.
DR   GO; GO:0006400; P:tRNA modification; IEA:HAMAP.
DR   HAMAP; MF_00290; -; 1.
DR   InterPro; IPR002905; TRM_MeTrfase.
DR   PANTHER; PTHR10631; TRM_mtfrase; 1.
DR   Pfam; PF02005; TRM; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Methyltransferase; S-adenosyl-L-methionine;
KW   Transferase; tRNA processing.
FT   CHAIN         1    363       N(2),N(2)-dimethylguanosine tRNA
FT                                methyltransferase.
FT                                /FTId=PRO_0000147688.
SQ   SEQUENCE   363 AA;  40108 MW;  7754A6FBFB41E9E7 CRC64;
     MRHLVLRREG GVKFYAPDPE KYGGIYSAPV FYNPAMEKNR TLSTLLLKAY GKGGLVVCEP
     LSGTGVRGIR YAVESGVVGK LILNDISKEA VELIKKNLEI NGVDAEVYNE DANVLLHKLK
     DSCDVVDIDP FGSPAPFIHG AFRALKEEGL ICATATDTAV LVGRYPRKCL RRYGSVIVKT
     PFYIEVGLRN LLGYIARVAA AEDYKITPLM SYWEGHYFRV CAYAARGARD ADDNFHHIAY
     IKYERGVRKI LHAQSEGSSG PLWVGPLGDP LIINKMSEIG PYQDFLKILA EEYSISAPWF
     YRLPEFAAGG KSPTLKEAVG VLRAAGIYAV RTHMAPDGFK ADGEYGEVLM AFKRYISSAH
     SLQ
//