Reviewed, UniProtKB/Swiss-Prot Q8ZWT5 (TRM1_PYRAE)

Names and origin

Protein names

Recommended name:
    N(2),N(2)-dimethylguanosine tRNA methyltransferase
    EC=2.1.1.32
Alternative name(s):
    tRNA(guanine-26,N(2)-N(2)) methyltransferase
    tRNA 2,2-dimethylguanosine-26 methyltransferase
    tRNA(m(2,2)G26)dimethyltransferase

Gene names

Name:	trm1
Ordered Locus Names:	PAE1628

Organism

Pyrobaculum aerophilum [Complete proteome] [HAMAP]

Taxonomic identifier

13773 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(2)-methylguanine. HAMAP MF_00290
Sequence similarities	Belongs to the TRM1 family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	N(2),N(2)-dimethylguanosine tRNA methyltransferase HAMAP MF_00290		PRO_0000147688

Sequences

	Sequence		Length	Mass (Da)
	Q8ZWT5-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 7754A6FBFB41E9E7	FASTA	363	40,108
	10 20 30 40 50 60 MRHLVLRREG GVKFYAPDPE KYGGIYSAPV FYNPAMEKNR TLSTLLLKAY GKGGLVVCEP 70 80 90 100 110 120 LSGTGVRGIR YAVESGVVGK LILNDISKEA VELIKKNLEI NGVDAEVYNE DANVLLHKLK 130 140 150 160 170 180 DSCDVVDIDP FGSPAPFIHG AFRALKEEGL ICATATDTAV LVGRYPRKCL RRYGSVIVKT 190 200 210 220 230 240 PFYIEVGLRN LLGYIARVAA AEDYKITPLM SYWEGHYFRV CAYAARGARD ADDNFHHIAY 250 260 270 280 290 300 IKYERGVRKI LHAQSEGSSG PLWVGPLGDP LIINKMSEIG PYQDFLKILA EEYSISAPWF 310 320 330 340 350 360 YRLPEFAAGG KSPTLKEAVG VLRAAGIYAV RTHMAPDGFK ADGEYGEVLM AFKRYISSAH SLQ

References

[1]

"Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum." Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I., Miller J.H. Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002) [PubMed: 11792869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE009831 Genomic DNA. Translation: AAL63614.1.
RefSeq	NP_559432.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1465863.
GenomeReviews	Gene locus PAE1628 in contig AE009441_GR.
KEGG	pai:PAE1628.
NMPDR	fig|178306.1.peg.1122.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8ZWT5.
OMA	Q8ZWT5. FHEDANV.
Enzyme and pathway databases
BioCyc	PAER178306:PAE1628-MON.
BRENDA	2.1.1.32. 142830.
Family and domain databases
HAMAP	MF_00290. [Tree]
InterPro	IPR002905. TRM_MeTrfase. [Graphical view]
PANTHER	PTHR10631. TRM_mtfrase. 1 hit.
Pfam	PF02005. TRM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRM1_PYRAE

Accession

Primary (citable) accession number: Q8ZWT5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2002
Last sequence update:	March 1, 2002
Last modified:	May 5, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)