Q8ZWK7 (G3P_PYRAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase
Short name=GAPDH
EC=1.2.1.59

Alternative name(s):
NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase

Gene names

Name:	gap
Ordered Locus Names:	PAE1740

Organism

Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)

Taxonomic identifier

178306 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H. HAMAP MF_00559
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. HAMAP MF_00559
Subunit structure	Homotetramer By similarity. HAMAP MF_00559
Subcellular location	Cytoplasm By similarity HAMAP MF_00559.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) (phosphorylating) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Glyceraldehyde-3-phosphate dehydrogenase HAMAP MF_00559		PRO_0000145729
Regions
	Nucleotide binding	11 – 12	2	NAD By similarity
	Region	139 – 141	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	195 – 196	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	140	1	Nucleophile By similarity
	Binding site	110	1	NAD; via amide nitrogen By similarity
	Binding site	169	1	NAD By similarity
	Binding site	302	1	NAD; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ZWK7 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: AFEC128B29907B1B	FASTA	344	37,671
	10 20 30 40 50 60 MIRVGIVGFG TIGKRIADAV AAQDDMKISG VLKVTPDYEA KIAVERGFKV YTLPDGLDKF 70 80 90 100 110 120 KKAGIEPAGT IEDLIEASDI IVDASPEDVG RENKEKYYIK FNKPVIFQGG EDADVAEVSF 130 140 150 160 170 180 NALANFEEAA GRRYVRVVSC NTTGITRVLS SLMLNGIKIK KARIFIARRG ADPKEHKKGP 190 200 210 220 230 240 INDVVPNPVT VPSHHGPDAQ TVLKNIDIVT MAVAVPVTIM HMHMAYLELD GSYTKEAVIE 250 260 270 280 290 300 AFAKTPRIFL ADVGAGFQSL AQIIEYARDL GRPRGDFPEV AVFRDSVTVN GNELYLMYGV 310 320 330 340 HQESIVVPEN IDAVRAVLGL APKWRSIEKT DKSLKLITEG KRYG

References

[1]	"Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum." Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I., Miller J.H. Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002) [PubMed: 11792869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009441 Genomic DNA. Translation: AAL63695.1.
RefSeq	NP_559513.1. NC_003364.1.
3D structure databases
ProteinModelPortal	Q8ZWK7.
SMR	Q8ZWK7. Positions 2-337.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1465945.
GenomeReviews	Gene locus PAE1740 in contig AE009441_GR.
KEGG	pai:PAE1740.
NMPDR	fig|178306.1.peg.1203.
Phylogenomic databases
HOGENOM	HBG392099.
OMA	VPSHHGP.
ProtClustDB	PRK04207.
Enzyme and pathway databases
BioCyc	PAER178306:PAE1740-MONOMER.
Family and domain databases
HAMAP	MF_00559. G3P_dehdrog_arch. [Tree]
InterPro	IPR020831. GlycerAld/Erythrose_P_DH. IPR020830. GlycerAld_3-P_DH_AS. IPR020829. GlycerAld_3-P_DH_cat. IPR020828. GlycerAld_3-P_DH_NAD(P)-bd. IPR006436. Glyceraldehyde-3-P_DH_2_arc. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00150.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
SMART	SM00846. Gp_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01546. GAPDH-II_archae. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P_PYRAE

Accession

Primary (citable) accession number: Q8ZWK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	March 1, 2002
Last modified:	November 16, 2011
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program