Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q8ZWI8 (HPPA_PYRAE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
K(+)-insensitive pyrophosphate-energized proton pump
EC=3.6.1.1
Alternative name(s):
Membrane-bound proton-translocating pyrophosphatase
Pyrophosphate-energized inorganic pyrophosphatase
Short name=H(+)-PPase
Gene names
Name:hppA
Ordered Locus Names:PAE1771
OrganismPyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)
Taxonomic identifier178306 [NCBI]
Taxonomic lineageArchaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum

Protein attributes

Sequence length721 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Proton pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for proton movement across the membrane. Generates a proton motive force By similarity. HAMAP MF_01129

Catalytic activity

Diphosphate + H2O = 2 phosphate. Ref.1

Cofactor

Magnesium. Ref.1

Subunit structure

Homodimer By similarity. HAMAP MF_01129

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity HAMAP MF_01129.

Sequence similarities

Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-insensitive subfamily. [View classification]

Biophysicochemical properties

Temperature dependence:

Optimum temperature is 80 degrees Celsius. Ref.1

Ontologies

Keywords
   Biological processHydrogen ion transport
Ion transport
Transport
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   LigandMagnesium
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processproton transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionhydrogen-translocating pyrophosphatase activity

Inferred from electronic annotation. Source: InterPro

inorganic diphosphatase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 721721K(+)-insensitive pyrophosphate-energized proton pump HAMAP MF_01129
PRO_0000217038

Regions

Transmembrane8 – 2821Helical; Potential
Transmembrane57 – 7721Helical; Potential
Transmembrane82 – 10221Helical; Potential
Transmembrane136 – 15621Helical; Potential
Transmembrane168 – 18821Helical; Potential
Transmembrane247 – 26721Helical; Potential
Transmembrane294 – 31421Helical; Potential
Transmembrane323 – 34321Helical; Potential
Transmembrane374 – 39421Helical; Potential
Transmembrane416 – 43621Helical; Potential
Transmembrane483 – 50321Helical; Potential
Transmembrane527 – 54721Helical; Potential
Transmembrane599 – 61921Helical; Potential
Transmembrane621 – 64121Helical; Potential
Transmembrane698 – 71821Helical; Potential

Sites

Site4761Determinant of potassium independence By similarity

Experimental info

Sequence conflict3051S → T in AAF01029. Ref.1
Sequence conflict5531A → P in AAF01029. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8ZWI8 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 56D9A64A998C7829

FASTA72175,394
        10         20         30         40         50         60 
MNMISYALLG VILGISGVIY AVYLAVWVLR QDPGNEKMRF ISQAIATGAR AYLFRQYRTL 

        70         80         90        100        110        120 
AVLLVILAVL ILVAIDMPRR TFGLTALAFI VGALGSMLAG YLGMYVTTRS ASRVAQAAAT 

       130        140        150        160        170        180 
GGMGKALLVS WRAGAVMGLS LASIALLLIS GFYLVFRSVL PDDWAVPLVA LGFGASLVTL 

       190        200        210        220        230        240 
FMRVGGGIYT KAADLGADLV GKVEAGIPED DPRNPGVIAD NVGDNVGDVA GMAADVYESY 

       250        260        270        280        290        300 
IVTVTAAIFL AAILGLPTQF IEAIILFAAL ALVATFAGVN LLKTTGVKHP LSSISLAIYA 

       310        320        330        340        350        360 
TIGLSVVLFF IGAFTLGLDS TKALALAATT SLGAVIAPLI VKITDYYTSY NYGPVRKIAE 

       370        380        390        400        410        420 
QAKISPATVI ITGYGVGLMS AIPVIAVIVA VLGISYMIGY YTVPVSGFGE LSKYLAGIFG 

       430        440        450        460        470        480 
TAMASVGLLV VAGIIITADS YGPVSDNAGG VVEMAGLPDE VREITDVLDS VGNTTKATTK 

       490        500        510        520        530        540 
GYAIASAALA ALVLFIALIF EIVYSASKIL GKGIVDMISE SLSGLQLINA NVLIGAFLGV 

       550        560        570        580        590        600 
ALVYFFSSRT LEAVGRTAME IVEEIRRQFR EKPGILEWKE QPDYARVVDI ATRRALGEFL 

       610        620        630        640        650        660 
IPGLAAIVLP LITGLLLGWN ALAGLIMGAI VAGVPRALLM ANAGGAWDNA KKYIEIQGLK 

       670        680        690        700        710        720 
KTEMHKAAVI GDTVGDPMKD TVGPSLNPLI KVLNTLSVVF TYVIVSTNIA LGIWPSGLLP 


F

« Hide

References

« Hide 'large scale' references
[1]"A thermostable vacuolar-type membrane pyrophosphatase from the archaeon Pyrobaculum aerophilum: implications for the origins of pyrophosphate-energized pumps."
Drozdowicz Y.M., Lu Y.-P., Patel V., Fitz-Gibbon S., Miller J.H., Rea P.A.
FEBS Lett. 460:505-512(1999) [PubMed: 10556526] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES.
Strain: ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827.
[2]"Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum."
Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I., Miller J.H.
Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002) [PubMed: 11792869] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF182812 Genomic DNA. Translation: AAF01029.1.
AE009441 Genomic DNA. Translation: AAL63714.1.
RefSeqNP_559532.1. NC_003364.1.

3D structure databases

ProteinModelPortalQ8ZWI8.
ModBaseSearch...

Protein family/group databases

TCDB3.A.10.3.1. H+-translocating pyrophosphatase (H+-PPase) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1465965.
GenomeReviewsGene locus PAE1771 in contig AE009441_GR.
KEGGpai:PAE1771.
NMPDRfig|178306.1.peg.1222.

Phylogenomic databases

HOGENOMHBG593668.
OMAILEWKEQ.
ProtClustDBPRK00733.

Enzyme and pathway databases

BioCycPAER178306:PAE1771-MONOMER.
BRENDA3.6.1.1. 5239.

Family and domain databases

HAMAPMF_01129. PPase-energized_pump.
[Tree]
InterProIPR004131. PPase-energised_H-pump.
[Graphical view]
KOK01507.
PfamPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsTIGR01104. V_PPase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHPPA_PYRAE
AccessionPrimary (citable) accession number: Q8ZWI8
Secondary accession number(s): Q9V2S8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: March 1, 2002
Last modified: November 16, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents