Q8ZW77 (SYY2_PYRAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine--tRNA ligase 2
EC=6.1.1.1

Alternative name(s):
Tyrosyl-tRNA synthetase 2
Short name=TyrRS 2

Gene names

Name:	tyrS2
Ordered Locus Names:	PAE1931

Organism

Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)

Taxonomic identifier

178306 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity. HAMAP MF_02009
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02009
Subunit structure	Homodimer By similarity. HAMAP MF_02009
Subcellular location	Cytoplasm By similarity HAMAP MF_02009.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 4 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	Tyrosine--tRNA ligase 2 HAMAP MF_02009		PRO_0000240267
Regions
	Motif	219 – 223	5	"KMSKS" region HAMAP MF_02009
Sites
	Binding site	26	1	Tyrosine By similarity
	Binding site	146	1	Tyrosine By similarity
	Binding site	150	1	Tyrosine By similarity
	Binding site	153	1	Tyrosine By similarity
	Binding site	168	1	Tyrosine By similarity
	Binding site	222	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ZW77 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: D9CDE761F1F6B890	FASTA	316	36,004
	10 20 30 40 50 60 MERLLMNVEE VVTREEFLRL KGGSAYLGFE PLWPIHIGWL IWAYKLAELK EAGFDVIVLV 70 80 90 100 110 120 ATWHAWINDK GSIEELRAHG ERVRAVLDRI GKFKYVYGDD VAKDPKYWEL VVKIAKETSL 130 140 150 160 170 180 ARVKRATPVM GRRAEEVELD FSKLMYPLMQ VADIFYLGVD VAVGGMDQRR AHMLARDVAE 190 200 210 220 230 240 KLGLKKPIAL HTPIITSLSG TGRMEGTHRE IDEVYAMYKM SKSKPQSAIL ITDSEEDVRK 250 260 270 280 290 300 KIWAAYCPPR ETKFNPVFEI AAYLLIPYHG PLEIKGRRYE EGNALERDYR EGVVTPQELK 310 EAVASALVGL LSKLKL

References

[1]

"Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum." Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I., Miller J.H. Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002) [PubMed: 11792869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009441 Genomic DNA. Translation: AAL63825.1.
RefSeq	NP_559643.1. NC_003364.1.
3D structure databases
ProteinModelPortal	Q8ZW77.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1464148.
GenomeReviews	Gene locus PAE1931 in contig AE009441_GR.
KEGG	pai:PAE1931.
NMPDR	fig|178306.1.peg.1333.
Phylogenomic databases
HOGENOM	HBG330667.
OMA	AWINDKG.
ProtClustDB	PRK08560.
Enzyme and pathway databases
BioCyc	PAER178306:PAE1931-MONOMER.
Family and domain databases
HAMAP	MF_02009. Tyr_tRNA_synth_type4. [Tree]
InterPro	IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002307. Tyr-tRNA-synth. IPR023678. Tyr-tRNA-synth_4. IPR023617. Tyr-tRNA-synth_arc-type. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01866.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PIRSF	PIRSF006588. TyrRS_arch_euk. 1 hit.
PRINTS	PR01040. TRNASYNTHTYR.
TIGRFAMs	TIGR00234. TyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY2_PYRAE

Accession

Primary (citable) accession number: Q8ZW77

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program