Q8ZVR7 (RIFK_PYRAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin kinase
Short name=RFK
EC=2.7.1.161

Alternative name(s):
CTP-dependent riboflavin kinase
CTP:riboflavin 5'-phosphotransferase
Flavokinase

Gene names

Name:	ribK
Ordered Locus Names:	PAE2157

Organism

Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)

Taxonomic identifier

178306 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity. HAMAP MF_01285
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01285
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW riboflavin kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 210	210	Riboflavin kinase HAMAP MF_01285		PRO_0000322099
Regions
	Nucleotide binding	91 – 96	6	CDP By similarity
	Nucleotide binding	190 – 193	4	CDP By similarity
	Region	1 – 81	81	H-T-H motif-like HAMAP MF_01285
	Region	82 – 210	129	Riboflavin kinase HAMAP MF_01285
Sites
	Metal binding	120	1	Magnesium By similarity
	Metal binding	122	1	Magnesium By similarity
	Binding site	177	1	FMN By similarity
	Binding site	185	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ZVR7 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 7E4D8285922A5950	FASTA	210	23,377
	10 20 30 40 50 60 MECKERRLIG DLIALSYVEG LPVAEAAKRL CVTRQGLYKL LKQLRNEGYV AEGPLIKITQ 70 80 90 100 110 120 KGRDLLSSVL RDLLRYFNIA SIRLIGRVIS GLGEGAFYIS LEGYRRAIEE KLGFTPFPGT 130 140 150 160 170 180 LNIKLDPQYL PYRRYLDGLP GIVIPGFTNG LRTYGGVKAF KAKINGVEGA VVMPERTHHP 190 200 210 TDVIEIIAPV KLRDALNLKD GDIVEVEILL

References

[1]

"Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum." Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I., Miller J.H. Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002) [PubMed: 11792869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009441 Genomic DNA. Translation: AAL63989.1.
RefSeq	NP_559807.1. NC_003364.1.
3D structure databases
ProteinModelPortal	Q8ZVR7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1464322.
GenomeReviews	Gene locus PAE2157 in contig AE009441_GR.
KEGG	pai:PAE2157.
NMPDR	fig|178306.1.peg.1497.
Phylogenomic databases
HOGENOM	HBG553349.
OMA	MPERTHH.
ProtClustDB	CLSK631895.
Enzyme and pathway databases
BioCyc	PAER178306:PAE2157-MONOMER.
Family and domain databases
HAMAP	MF_01285. Riboflavin_kinase. Fused. [Tree]
InterPro	IPR023470. Riboflavin_kinase_archaeal. IPR023602. Riboflavin_kinase_CTP-dep. IPR023465. Riboflavin_kinase_domain. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.40.30.30. Riboflavin_kinase. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K07732.
Pfam	PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIFK_PYRAE

Accession

Primary (citable) accession number: Q8ZVR7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	March 1, 2002
Last modified:	November 16, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program