Reviewed, UniProtKB/Swiss-Prot Q8ZVR7 (RIFK_PYRAE)

Names and origin

Protein names

Recommended name:
    Riboflavin kinase
      Short name=RFK
    EC=2.7.1.161
Alternative name(s):
    CTP:riboflavin 5'-phosphotransferase
    CTP-dependent riboflavin kinase
    Flavokinase

Gene names

Name:	rfk
Ordered Locus Names:	PAE2157

Organism

Pyrobaculum aerophilum [Complete proteome] [HAMAP]

Taxonomic identifier

13773 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity. HAMAP MF_01285
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01285
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	FMN biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: HAMAP riboflavin kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 210	210	Riboflavin kinase HAMAP MF_01285		PRO_0000322099
Regions
	Nucleotide binding	91 – 96	6	CDP By similarity
	Nucleotide binding	190 – 193	4	CDP By similarity
	Region	1 – 81	81	H-T-H motif-like HAMAP MF_01285
	Region	82 – 210	129	Riboflavin kinase HAMAP MF_01285
Sites
	Metal binding	120	1	Magnesium By similarity
	Metal binding	122	1	Magnesium By similarity
	Binding site	177	1	FMN By similarity
	Binding site	185	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ZVR7-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 7E4D8285922A5950	FASTA	210	23,377
	10 20 30 40 50 60 MECKERRLIG DLIALSYVEG LPVAEAAKRL CVTRQGLYKL LKQLRNEGYV AEGPLIKITQ 70 80 90 100 110 120 KGRDLLSSVL RDLLRYFNIA SIRLIGRVIS GLGEGAFYIS LEGYRRAIEE KLGFTPFPGT 130 140 150 160 170 180 LNIKLDPQYL PYRRYLDGLP GIVIPGFTNG LRTYGGVKAF KAKINGVEGA VVMPERTHHP 190 200 210 TDVIEIIAPV KLRDALNLKD GDIVEVEILL

References

[1]

"Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum." Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I., Miller J.H. Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002) [PubMed: 11792869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009441 Genomic DNA. Translation: AAL63989.1.
RefSeq	NP_559807.1.
3D structure databases
SMR	Q8ZVR7. Positions 12-196, 80-210.
ModBase	Search...
Genome annotation databases
GeneID	1464322.
GenomeReviews	Gene locus PAE2157 in contig AE009441_GR.
KEGG	pai:PAE2157.
NMPDR	fig|178306.1.peg.1497.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG553349.
OMA	MPERTHH.
Enzyme and pathway databases
BioCyc	PAER178306:PAE2157-MONOMER.
Family and domain databases
HAMAP	MF_01285. Riboflavin_kinase. Fused. [Tree]
InterPro	IPR002834. Riboflavin_kinase_arc. [Graphical view]
Pfam	PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIFK_PYRAE

Accession

Primary (citable) accession number: Q8ZVR7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	March 1, 2002
Last modified:	January 19, 2010
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)