Q8ZVM1 (PSA_PYRAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proteasome subunit alpha
EC=3.4.25.1

Alternative name(s):
20S proteasome alpha subunit
Proteasome core protein PsmA

Gene names

Name:	psmA
Ordered Locus Names:	PAE2215

Organism

Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827) [Reference proteome] [HAMAP]

Taxonomic identifier

178306 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum › Pyrobaculum aerophilum

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation By similarity. HAMAP-Rule MF_00289
Catalytic activity	Cleavage of peptide bonds with very broad specificity. HAMAP-Rule MF_00289
Enzyme regulation	The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity By similarity. HAMAP-Rule MF_00289
Subunit structure	The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN By similarity.
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00289.
Sequence similarities	Belongs to the peptidase T1A family.

Ontologies

Keywords
Cellular component	Cytoplasm Proteasome
Molecular function	Hydrolase Protease Threonine protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell proteasome core complex, alpha-subunit complex Inferred from electronic annotation. Source: InterPro
Molecular_function	threonine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 243	243	Proteasome subunit alpha HAMAP-Rule MF_00289		PRO_0000124182

Sequences

	Sequence		Length	Mass (Da)
	Q8ZVM1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 53D9907408AA61A9	FASTA	243	26,626
	10 20 30 40 50 60 MFPPAMAGYD RAITIFSPEG KIYQVEYAGE AVKRGWPTVG VKCKAGVVLT AEKRKISALF 70 80 90 100 110 120 DSSSLEKIYL IDEHVAASPS GLLADARILI DYARDVALSH RFIYDEPIDV EFLTKAVCNL 130 140 150 160 170 180 KQQYTQFGGA RPFGVALLIA GVDRHGARLY QTDPSGVYIG YFATAIGAES GTITEFLEKN 190 200 210 220 230 240 YKFDMEMGEC IELALKALAS AVEITDSANV EVAYATIEEK KMRKMSQDEV ASLLTKLGLL KKS

References

[1]

"Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum." Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I., Miller J.H. Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009441 Genomic DNA. Translation: AAL64035.1.
RefSeq	NP_559853.1. NC_003364.1.
3D structure databases
ProteinModelPortal	Q8ZVM1.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
STRING	178306.PAE2215.
Protein family/group databases
MEROPS	T01.973.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL64035; AAL64035; PAE2215.
GeneID	1464369.
KEGG	pai:PAE2215.
Phylogenomic databases
eggNOG	COG0638.
HOGENOM	HOG000091085.
KO	K03432.
OMA	FQVNYAR.
Enzyme and pathway databases
BioCyc	PAER178306:GCEO-1595-MONOMER.
Family and domain databases
Gene3D	3.60.20.10. 1 hit.
HAMAP	MF_00289_A. Proteasome_A_A.
InterPro	IPR029055. Ntn_hydrolases_N. IPR019982. Proteasome_asu_arc. IPR000426. Proteasome_asu_N. IPR023332. Proteasome_suA-type. IPR001353. Proteasome_sua/b. [Graphical view]
Pfam	PF00227. Proteasome. 1 hit. PF10584. Proteasome_A_N. 1 hit. [Graphical view]
SMART	SM00948. Proteasome_A_N. 1 hit. [Graphical view]
SUPFAM	SSF56235. SSF56235. 1 hit.
TIGRFAMs	TIGR03633. arc_protsome_A. 1 hit.
PROSITE	PS00388. PROTEASOME_ALPHA_1. 1 hit. PS51475. PROTEASOME_ALPHA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSA_PYRAE

Accession

Primary (citable) accession number: Q8ZVM1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2002
Last sequence update:	March 1, 2002
Last modified:	June 11, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program