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Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.UniRule annotation

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei120UniRule annotation1
Active sitei128UniRule annotation1
Active sitei159UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processtRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
Alternative name(s):
tRNA-intron endonucleaseUniRule annotation
Gene namesi
Name:endAUniRule annotation
Ordered Locus Names:PAE2269
OrganismiPyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)
Taxonomic identifieri178306 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum
Proteomesi
  • UP000002439 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001094761 – 183tRNA-splicing endonucleaseAdd BLAST183

Interactioni

Subunit structurei

Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi178306.PAE2269

Structurei

Secondary structure

1183
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 5Combined sources4
Beta strandi7 – 12Combined sources6
Helixi15 – 23Combined sources9
Beta strandi28 – 30Combined sources3
Helixi38 – 43Combined sources6
Beta strandi48 – 51Combined sources4
Helixi52 – 60Combined sources9
Beta strandi65 – 67Combined sources3
Helixi76 – 86Combined sources11
Helixi90 – 102Combined sources13
Beta strandi106 – 109Combined sources4
Helixi111 – 113Combined sources3
Beta strandi115 – 122Combined sources8
Beta strandi124 – 128Combined sources5
Beta strandi131 – 135Combined sources5
Helixi144 – 153Combined sources10
Beta strandi161 – 166Combined sources6
Turni168 – 170Combined sources3
Beta strandi173 – 180Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZYZX-ray1.70B/D1-183[»]
ProteinModelPortaliQ8ZVI1
SMRiQ8ZVI1
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8ZVI1

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01701 Archaea
COG1676 LUCA
HOGENOMiHOG000107823
InParanoidiQ8ZVI1
KOiK01170
OMAiKYGSDFR
OrthoDBiPOG093Z0DMC

Family and domain databases

Gene3Di3.40.1350.10, 1 hit
HAMAPiMF_01833 EndA_short, 1 hit
InterProiView protein in InterPro
IPR011856 tRNA_endonuc-like_dom_sf
IPR036167 tRNA_intron_Endo_cat-like_sf
IPR006677 tRNA_intron_Endonuc_cat-like
IPR006678 tRNA_intron_Endonuc_N
IPR036740 tRNA_intron_Endonuc_N_sf
IPR006676 tRNA_splic
IPR016442 tRNA_splic_arch_short
PANTHERiPTHR21227 PTHR21227, 2 hits
PfamiView protein in Pfam
PF01974 tRNA_int_endo, 1 hit
PF02778 tRNA_int_endo_N, 1 hit
PIRSFiPIRSF005285 tRNA_splic_archaea, 1 hit
SUPFAMiSSF53032 SSF53032, 1 hit
SSF55267 SSF55267, 1 hit
TIGRFAMsiTIGR00324 endA, 1 hit

Sequencei

Sequence statusi: Complete.

Q8ZVI1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIGYLRGLAV IVEDVEFARR LYKEGFYGRF LGYDKVKRDE VEKINAPLIL
60 70 80 90 100
GLYEALYLAE KGRLKVMGED GREVAPEELA ALGRERMRNF DEIYKIYKYF
110 120 130 140 150
RDLGYVVKSG LKFGALFSVY EKGPGIDHAP MVVVFLEPDK GISATDITRG
160 170 180
GRLSHSVRKT WTLATVLRQT GEVVLLGFGW ARL
Length:183
Mass (Da):20,775
Last modified:March 1, 2002 - v1
Checksum:iE59B9BAD7352AD33
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009441 Genomic DNA Translation: AAL64075.1

Genome annotation databases

EnsemblBacteriaiAAL64075; AAL64075; PAE2269
KEGGipai:PAE2269
PATRICifig|178306.9.peg.1689

Similar proteinsi

Entry informationi

Entry nameiENDA_PYRAE
AccessioniPrimary (citable) accession number: Q8ZVI1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: March 1, 2002
Last modified: March 28, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health