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Protein

Bifunctional NAD(P)H-hydrate repair enzyme Nnr

Gene

nnr

Organism
Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration (By similarity).By similarity

Catalytic activityi

ADP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide adenine-dinucleotide = AMP + phosphate + NADH.
ADP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide adenine-dinucleotide phosphate = AMP + phosphate + NADPH.
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide.
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate.

Cofactori

K+By similarityNote: Binds 1 potassium ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi57PotassiumBy similarity1
Metal bindingi127PotassiumBy similarity1
Binding sitei160NAD(P)HX (for epimerase activity)By similarity1
Metal bindingi163PotassiumBy similarity1
Binding sitei323NAD(P)HX (for dehydratase activity); via amide nitrogenBy similarity1
Binding sitei437NAD(P)HX (for dehydratase activity)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi408 – 412ADPBy similarity5
Nucleotide bindingi427 – 436ADPBy similarity10

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Lyase, Multifunctional enzyme
LigandATP-binding, Metal-binding, NAD, NADP, Nucleotide-binding, Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional NAD(P)H-hydrate repair enzyme Nnr
Alternative name(s):
Nicotinamide nucleotide repair protein
Including the following 2 domains:
ADP-dependent (S)-NAD(P)H-hydrate dehydratase (EC:4.2.1.136)
Alternative name(s):
ADP-dependent NAD(P)HX dehydratase
NAD(P)H-hydrate epimerase (EC:5.1.99.6)
Alternative name(s):
NAD(P)HX epimerase
Gene namesi
Name:nnr
Ordered Locus Names:PAE2520
OrganismiPyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)
Taxonomic identifieri178306 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum
Proteomesi
  • UP000002439 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004164311 – 501Bifunctional NAD(P)H-hydrate repair enzyme NnrAdd BLAST501

Proteomic databases

PRIDEiQ8ZV04

Interactioni

Protein-protein interaction databases

STRINGi178306.PAE2520

Structurei

3D structure databases

ProteinModelPortaliQ8ZV04
SMRiQ8ZV04
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 217YjeF N-terminalAdd BLAST209
Domaini220 – 495YjeF C-terminalAdd BLAST276

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 221NAD(P)H-hydrate epimeraseBy similarityAdd BLAST221
Regioni56 – 60NAD(P)HX (for epimerase activity)By similarity5
Regioni131 – 137NAD(P)HX (for epimerase activity)By similarity7
Regioni221 – 501ADP-dependent (S)-NAD(P)H-hydrate dehydrataseBy similarityAdd BLAST281
Regioni371 – 377NAD(P)HX (for dehydratase activity)By similarity7

Sequence similaritiesi

In the N-terminal section; belongs to the NnrE/AIBP family.Curated
In the C-terminal section; belongs to the NnrD/CARKD family.Curated

Phylogenomic databases

eggNOGiarCOG00018 Archaea
ENOG4102T90 Archaea
COG0062 LUCA
COG0063 LUCA
HOGENOMiHOG000228404
InParanoidiQ8ZV04
KOiK17758
K17759
OMAiLVGPGHN
OrthoDBiPOG093Z0AXM

Family and domain databases

CDDicd01171 YXKO-related, 1 hit
Gene3Di3.40.1190.20, 1 hit
3.40.50.10260, 1 hit
HAMAPiMF_01965 NADHX_dehydratase, 1 hit
MF_01966 NADHX_epimerase, 1 hit
InterProiView protein in InterPro
IPR017953 Carbohydrate_kinase_pred_CS
IPR000631 CARKD
IPR030677 Nnr
IPR029056 Ribokinase-like
IPR004443 YjeF_N_dom
IPR036652 YjeF_N_dom_sf
PfamiView protein in Pfam
PF01256 Carb_kinase, 1 hit
PF03853 YjeF_N, 1 hit
PIRSFiPIRSF017184 Nnr, 1 hit
SUPFAMiSSF53613 SSF53613, 1 hit
SSF64153 SSF64153, 1 hit
TIGRFAMsiTIGR00196 yjeF_cterm, 1 hit
TIGR00197 yjeF_nterm, 1 hit
PROSITEiView protein in PROSITE
PS01050 YJEF_C_2, 1 hit
PS51383 YJEF_C_3, 1 hit
PS51385 YJEF_N, 1 hit

Sequencei

Sequence statusi: Complete.

Q8ZV04-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESITSIEMY VADRNAEWLG VPRLVLMENA GAAVARNVLR KFPTAKRILV
60 70 80 90 100
VCGTGDNGGD GYVAARHLHA AGRTVRVIAL GEPREELARI NYQAVTRLWG
110 120 130 140 150
VEVKAAQLPL ELLALQDWFM WAEVIVDAVL GTGIRGVLRE PHATAIELMN
160 170 180 190 200
ISPAPKVAVD VPSGLDPDTG EVRDKAVRAA LTVTFHKAKR GLLAPGAQRY
210 220 230 240 250
VGELVVEPIG IPPEAELVVG PGDFAYLNFT RRADSKKGDH GRVLVIGGSL
260 270 280 290 300
EYSGAPVYVA LAALRAGVDL AVIAAPEPAA YAAKAISPDI IAIPLEGPRL
310 320 330 340 350
STKHVDKLAS LAERFNVVAM GPGLGVEEET QEAVRELFRR LAGKRAMVID
360 370 380 390 400
ADALKALRGV RASGAVVYTP HAGEFKALTG AEPPQSLSER MAVVREQAAA
410 420 430 440 450
LGGVILLKGR YDVISDGVRV KVNMTGTPAM TVGGTGDVLT GLVAAFLTKT
460 470 480 490 500
SDPLEAAAVA AFVNGLAGED AAAELGFHIT ASDLIERLPR VIRRYAFESI

R
Length:501
Mass (Da):53,031
Last modified:March 1, 2002 - v1
Checksum:iB8A0D79AC45E638D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009441 Genomic DNA Translation: AAL64252.1

Genome annotation databases

EnsemblBacteriaiAAL64252; AAL64252; PAE2520
KEGGipai:PAE2520
PATRICifig|178306.9.peg.1877

Similar proteinsi

Entry informationi

Entry nameiNNR_PYRAE
AccessioniPrimary (citable) accession number: Q8ZV04
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: March 1, 2002
Last modified: May 23, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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