Q8ZU79 (SURE1_PYRAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5'-nucleotidase surE1
EC=3.1.3.5

Alternative name(s):
Nucleoside 5'-monophosphate phosphohydrolase 1

Gene names

Name:	surE1
Ordered Locus Names:	PAE2908

Organism

Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)

Taxonomic identifier

178306 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Nucleotidase that shows the greatest phosphatase activity on purine (deoxy)nucleoside 5'-monophosphates, particularly the substrates 5'-GMP, 5'-AMP and 2'-deoxy-5'-AMP. Ref.2
Catalytic activity	A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate. HAMAP MF_00060
Cofactor	Binds 1 divalent metal cation per subunit. Most active with Co2+, and may utilize Mg2+ or Mn2+, though with less activity. Ref.2
Subunit structure	Homodimer. Ref.2
Subcellular location	Cytoplasm Potential HAMAP MF_00060.
Sequence similarities	Belongs to the surE nucleotidase family.
Caution	Was originally annotated as an acid phosphatase (EC 3.1.3.2).
Biophysicochemical properties	Temperature dependence: Optimum temperature is above 90 degrees Celsius. HAMAP MF_00060

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Cobalt Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	5'-nucleotidase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 266

266

5'-nucleotidase surE1 HAMAP MF_00060

PRO_0000111869

Sites

Metal binding

8

1

Divalent metal cation By similarity

Metal binding

9

1

Divalent metal cation By similarity

Metal binding

39

1

Divalent metal cation By similarity

Metal binding

93

1

Divalent metal cation By similarity

Secondary structure

1

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266

Helix Strand Turn

Details...

Beta strand

2 – 6

5

Helix

14 – 23

10

Helix

24 – 26

3

Beta strand

27 – 36

10

Turn

38 – 40

3

Beta strand

53 – 57

5

Beta strand

59 – 68

10

Helix

70 – 81

12

Beta strand

86 – 95

10

Helix

100 – 103

4

Helix

107 – 117

11

Beta strand

122 – 127

6

Helix

133 – 136

4

Helix

139 – 159

21

Beta strand

166 – 171

6

Beta strand

182 – 184

3

Beta strand

197 – 200

4

Beta strand

206 – 209

4

Helix

222 – 227

6

Beta strand

232 – 237

6

Beta strand

242 – 244

3

Sequences

	Sequence		Length	Mass (Da)
	Q8ZU79 [UniParc]. Last modified March 5, 2002. Version 1. Checksum: 292677D5D59149D0	FASTA	266	28,845
	10 20 30 40 50 60 MKILVTNDDG VHSPGLRLLY QFALSLGDVD VVAPESPKSA TGLGITLHKP LRMYEVDLCG 70 80 90 100 110 120 FRAIATSGTP SDTVYLATFG LGRKYDIVLS GINLGDNTSL QVILSSGTLG AAFQAALLGI 130 140 150 160 170 180 PALAYSAYLE NWNELLNNKE AVEIMGAVVS STASYVLKNG MPQGVDVISV NFPRRLGRGV 190 200 210 220 230 240 RAKLVKAAKL RYAQQVVERV DPRGVRYYWL YGRDLAPEPE TDVYVVLKEG GIAITPLTLN 250 260 LNAVDAHREV DMDSLNRMVE YINASL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum." Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I., Miller J.H. Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002) [PubMed: 11792869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827.
[2]	"Structure and function of an archaeal homolog of survival protein E (SurEalpha): an acid phosphatase with purine nucleotide specificity." Mura C., Katz J.E., Clarke S.G., Eisenberg D. J. Mol. Biol. 326:1559-1575(2003) [PubMed: 12595266] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), FUNCTION, CHARACTERIZATION, COFACTOR, SUBUNIT, TEMPERATURE DEPENDENCE.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE009441 Genomic DNA. Translation: AAL64529.1.

RefSeq

NP_560347.1. NC_003364.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1L5X	X-ray	2.00	A/B	1-266	[»]

ProteinModelPortal

Q8ZU79.

SMR

Q8ZU79. Positions 1-266.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1463691.

GenomeReviews

Gene locus PAE2908 in contig AE009441_GR.

KEGG

pai:PAE2908.

NMPDR

fig|178306.1.peg.2037.

Phylogenomic databases

HOGENOM

HBG600532.

OMA

HAPALIR.

ProtClustDB

PRK13934.

Enzyme and pathway databases

BioCyc

PAER178306:PAE2908-MONOMER.

Family and domain databases

HAMAP

MF_00060. SurE.
[Tree]

InterPro

IPR002828. SurE-like_Pase/nucleotidase.
[Graphical view]

Gene3D

G3DSA:3.40.1210.10. SurE-like_Pase/nucleotidase. 1 hit.

KO

K03787.

Pfam

PF01975. SurE. 1 hit.
[Graphical view]

SUPFAM

SSF64167. SurE-like_Pase/nucleotidase. 1 hit.

TIGRFAMs

TIGR00087. SurE. 1 hit.

ProtoNet

Search...

Entry information

Entry name

SURE1_PYRAE

Accession

Primary (citable) accession number: Q8ZU79

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	March 5, 2002
Last modified:	December 14, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program