Q8ZTE1 (ILVC_PYRAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	PAE3298

Organism

Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)

Taxonomic identifier

178306 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_0000151397
Sites
	Active site	108	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8ZTE1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: BD984EDE8F6B7CC2	FASTA	328	36,178
	10 20 30 40 50 60 MAKIYTDREA SLEPLKGKTI AVIGYGIQGR AQALNLRDSG LEVIIGLRRG GKSWELATSE 70 80 90 100 110 120 GFRVYEIGEA VRKADVILVL IPDMEQPKVW QEQIAPNLKE GVVVDFAHGF NVHFGLIKPP 130 140 150 160 170 180 KNIDVIMVAP KAPGKAVREE YLAGRGVPAL VAVYQDYSGS ALKYALALAK GIGATRAGVI 190 200 210 220 230 240 ETTFAEETET DLIGEQIVLV GGLMELIKKG FEVLVEMGYQ PEVAYFEVLN EAKLIMDLIW 250 260 270 280 290 300 QRGIYGMLNG VSDTAKYGGL TVGPRVIDEN VKRKMKEAAM RVKSGEFAKE WVEEYNRGAP 310 320 TLRKLMEEAR THPIEKVGEE MRKLLFGP

References

[1]	"Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum." Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I., Miller J.H. Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002) [PubMed: 11792869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009441 Genomic DNA. Translation: AAL64821.1.
RefSeq	NP_560639.1. NC_003364.1.
3D structure databases
ProteinModelPortal	Q8ZTE1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1464010.
GenomeReviews	Gene locus PAE3298 in contig AE009441_GR.
KEGG	pai:PAE3298.
NMPDR	fig|178306.1.peg.2329.
Phylogenomic databases
HOGENOM	HBG286525.
OMA	MAYAKGI.
ProtClustDB	PRK05479.
Enzyme and pathway databases
BioCyc	PAER178306:PAE3298-MONOMER.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. [Graphical view]
Gene3D	G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00053.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_PYRAE

Accession

Primary (citable) accession number: Q8ZTE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2003
Last sequence update:	March 1, 2002
Last modified:	December 14, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program