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Protein

CCA-adding enzyme

Gene

cca

Organism
Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.UniRule annotation

Catalytic activityi

A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei54 – 541ATP or CTPUniRule annotation
Binding sitei57 – 571ATP or CTPUniRule annotation
Metal bindingi66 – 661MagnesiumUniRule annotation
Metal bindingi68 – 681MagnesiumUniRule annotation
Metal bindingi118 – 1181MagnesiumUniRule annotation
Binding sitei141 – 1411ATP or CTPUniRule annotation
Binding sitei161 – 1611ATP or CTPUniRule annotation
Binding sitei170 – 1701ATP or CTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

RNA repair, tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciPAER178306:GCEO-2436-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CCA-adding enzymeUniRule annotation (EC:2.7.7.72UniRule annotation)
Alternative name(s):
CCA tRNA nucleotidyltransferaseUniRule annotation
tRNA CCA-pyrophosphorylaseUniRule annotation
tRNA adenylyl-/cytidylyl- transferaseUniRule annotation
tRNA nucleotidyltransferaseUniRule annotation
tRNA-NTUniRule annotation
Gene namesi
Name:ccaUniRule annotation
Ordered Locus Names:PAE3325
OrganismiPyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)
Taxonomic identifieri178306 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum
Proteomesi
  • UP000002439 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 419419CCA-adding enzymePRO_0000139077Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi178306.PAE3325.

Structurei

3D structure databases

ProteinModelPortaliQ8ZTC3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04249. Archaea.
COG1746. LUCA.
HOGENOMiHOG000226400.
InParanoidiQ8ZTC3.
KOiK07558.
OMAiKGTWLKG.

Family and domain databases

HAMAPiMF_01264. CCA_arch.
InterProiIPR008229. CCA-adding_arc.
IPR011068. NuclTrfase_I_C.
IPR002934. Polymerase_NTP_transf_dom.
IPR015329. tRNA_NucTransf2.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF09249. tRNA_NucTransf2. 1 hit.
[Graphical view]
PIRSFiPIRSF005335. CCA_arch. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.
TIGRFAMsiTIGR03671. cca_archaeal. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8ZTC3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTLEEVLSE AYKLVIPSEE EEKRIREVTQ KVKRLVSQII EEGGIDALVD
60 70 80 90 100
VYGSGARGTW LPGQRDIDIF VVLNDRRIKP EDVVKILTSR FTTLGLNWAL
110 120 130 140 150
RYAQHPYVSL QVDDYEVDIV PCYKIQPGER PITAADRSPL HHKFLSERLK
160 170 180 190 200
KDQILDVRLL KLFLKTIGVY GAEIKTEGFS GYLTELLVVY YGSFIEVLRA
210 220 230 240 250
ASRWRPYKTY ITFVETSAKF KSPLVVVDPV DPNRNAAAAV SLTSMSTLIL
260 270 280 290 300
AARRFLKKPS LSYFQPSRGY AIRQVETVEV VYPYPGEPPD IVWGKYKRIG
310 320 330 340 350
RNLFKWLREC GFKVFRWGVE SDEKTYVKLV YVVEQTKLAP YTLHKGPPVY
360 370 380 390 400
DDAVDAFIEK YVNEEVIGPF VVGARAYVIK KRKWTDISHC INAKLGKGNY
410
DIRVNLYDGD LVRKTPWLT
Length:419
Mass (Da):48,046
Last modified:March 1, 2002 - v1
Checksum:iDE0613CC00C8FDE9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009441 Genomic DNA. Translation: AAL64839.1.

Genome annotation databases

EnsemblBacteriaiAAL64839; AAL64839; PAE3325.
KEGGipai:PAE3325.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009441 Genomic DNA. Translation: AAL64839.1.

3D structure databases

ProteinModelPortaliQ8ZTC3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi178306.PAE3325.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL64839; AAL64839; PAE3325.
KEGGipai:PAE3325.

Phylogenomic databases

eggNOGiarCOG04249. Archaea.
COG1746. LUCA.
HOGENOMiHOG000226400.
InParanoidiQ8ZTC3.
KOiK07558.
OMAiKGTWLKG.

Enzyme and pathway databases

BioCyciPAER178306:GCEO-2436-MONOMER.

Family and domain databases

HAMAPiMF_01264. CCA_arch.
InterProiIPR008229. CCA-adding_arc.
IPR011068. NuclTrfase_I_C.
IPR002934. Polymerase_NTP_transf_dom.
IPR015329. tRNA_NucTransf2.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF09249. tRNA_NucTransf2. 1 hit.
[Graphical view]
PIRSFiPIRSF005335. CCA_arch. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.
TIGRFAMsiTIGR03671. cca_archaeal. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum."
    Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I., Miller J.H.
    Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827.

Entry informationi

Entry nameiCCA_PYRAE
AccessioniPrimary (citable) accession number: Q8ZTC3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: March 1, 2002
Last modified: November 11, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

A single active site specifically recognizes both ATP and CTP and is responsible for their addition.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.